Posted by admin on Aug 31, 2025 in |
Bioanalysis is most widely used for drug discovery and development. Bioanalytical methods have been working for the quantitative estimation of drugs, or its metabolites and biomarkers in its biological samples. The steps involved in bioanalysis comprise of many steps that are from sample collection to sample analysis and then data reporting. The first step involves sample collection from clinical or preclinical studies. After that, the analysis of the sample is done in the laboratory. The second step involves the sample cleanup the cleanup process should be carried out carefully to eliminate the interferences present in the sample matrix. And it increases the performance of the analytical system. The last step involves sample analysis and detection of a separated compound and detection of techniques used are HPLC and Liquid chromatography coupled with double mass spectrometry (LCMS-MS) and are used in the bioanalysis of drugs in the body. Each one of these has its advantage and disadvantage. For bioanalysis of small or large molecules the chromatographic methods used are HPLC, and...
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Posted by admin on Aug 31, 2024 in |
Ovarian cancer, a major concern in India, is among the leading causes of cancer-related deaths in women. Early detection is crucial due to frequent late-stage diagnoses. Red Cell Distribution Width (RDW), a marker for red blood cell size variability, is promising but lacks extensive study in ovarian cancer. This research aims to correlate RDW with hematological parameters in ovarian cancer patients, potentially improving early detection and prognosis. This study examined data from ovarian cancer patients at Mc Gann’s Teaching District Hospital in Shivamogga. Hematological parameters, RDW, were obtained from records, with imaging and histopathological analysis confirming diagnoses. Inclusion criteria Involved ovarian malignancies or benign tumors, while exclusions involved comorbidities or recent treatments impacting blood parameters. Significant differences were observed in RDW (p = 0.033), Platelet-to-Lymphocyte Ratio (PLR) (p = 0.004), Neutrophil-to-Lymphocyte Ratio (NLR) (p = 0.001), Lymphocyte count (L) (p = 0.0006), and Neutrophil count (N) (p = 0.003) among the case, control, and normal groups. Post hoc analysis revealed significant disparities between the case and control groups for...
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Posted by admin on Aug 31, 2024 in |
Silymarin, an intricate blend of flavonolignans obtained from Silybum marianum, or milk thistle, has drawn a lot of interest because of its possible medical uses. This study reports on the development and validation of an analytical technique that employs high performance thin layer chromatography (HPTLC) to estimate silymarin in pharmaceutical dosage forms (tablet) and bulk drug. Chloroform: methanol (6:4 v/v) was the solvent system used in the chromatographic separation of the medication on aluminium plates pre-coated with silica gel 60 GF254, (10 cm × 10 cm with 250 μm layer thickness) as the stationary phase. At 288 nm, a densitometric analysis of the divided zones was carried out. Densitometric scanning was carried out at 288 nm using a Camag TLC scanner 3, which was controlled by Win CATS software. The radiation source utilised for the scan was a deuterium lamp with slit size of 5.00 x 0.45 mm. It was discovered that the retention factor was 0.42. In the 200–1200 ng/band range, linearity R2= 0.993 was used to evaluate...
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Posted by admin on Aug 31, 2024 in |
Background: Breast cancer is commonest malignancy of women globally. A palpable breast lump is common diagnostic dilemma to surgeons. In 2016,International Academy of Cytology (IAC) established a standardized approach for reporting Breast FNAC by categorizing the lesions in five tier system from C1 to C5 to standardize reporting, which will enhance Breast FNAB cytology performance, interpretation, and reporting and its histopathological correlation to improve diagnostic accuracy. Aims and Objectives: To categorize FNAC cases according to The IAC Yokohama System and correlate histopathologically (wherever possible) and evaluate the diagnostic ability of FNAC. Material and Methods: The current prospective analytical study was carried out in the Department of Pathology, Shyam Shah Medical College, Rewa, Madhya Pradesh, for a period of 15 months. The results were analysed based on cytological and histopathological reporting. Results: The highest number of cases were from the age group of 21- 30 years with 29% (n=29). The maximum number of cases, 69% (n=69) of the total, were found to be categorized under the category C2 (benign) which...
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Posted by admin on Aug 31, 2024 in |
Introduction: Acne is characterized by non-inflammatory follicular papules or comedones and by inflammatory papules, pustules and nodules and is estimated to affect 9.4% of the global population. Topical combination therapy is the first-line approach for facial acne. Objectives: To compare the efficacy and safety of topical clindamycin 1% with tretinoin 0.025% and clindamycin 1% with nicotinamide 4% in mild to moderate acne vulgaris. Methods: This was a comparative study conducted at BMCRI including seventy subjects who were randomized (1:1) into two groups. Group A received clindamycin with Tretinoin and Group B received clindamycin with nicotinamide. Efficacy was assessed by mean change in Acne Severity Index (ASI) from baseline and at the end of 4, 8 and 12 weeks. Safety was assessed by adverse events reported. Results: Both treatment groups showed statistically significant improvement. Baseline ASI of Group A was 86.48 ± 4.49 and of Group B was 88.05 ± 4.02, which were comparable. At end of 12 weeks, ASI of Group A and Group B was reduced to 25.65±...
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