Posted by admin on Aug 31, 2018 in |
About 70 middle aged (20 – 45 years) patients, including 40 males and 30 females, operated at Base Military Hospital, Meerut Cantonment Board, Meerut (India) developed typical symptoms of pus formation at surgical sites and opening of sutures after 30 – 45 days. To investigate the causative pathogen and source of infection, various samples were collected from wounds and various sites of Operation Theatre. The samples were processed on culture media including nutrient agar, McConkey agar and Lowenstein-Jensen’s medium (LJ medium). To identify the pathogens, Gram’s and acid fast staining of the smears of samples and cultures obtained on different media, were carried out. Acid fast bacilli were observed after 3 – 5 days of incubation on LJ medium. The wound samples showed the prevalence of Mycobacterium chelonae and acid fast...
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Posted by admin on Aug 31, 2018 in |
A simple, sensitive, and high-performance liquid chromatography ultraviolet detection (HPLC-UV) method was developed and validated for the quantification of palbociclib in human plasma. Plasma samples were processed by solid phase extraction using an Oasis hydrophilic-lipophilic balance extraction cartridge (1 mL, 30 mg). Commercial imatinib was used as an internal standard. Palbociclib and imatinib (IS) in human plasma were analyzed using a mobile phase of acetonitrile and 0.1% Triethylamine TEA (pH 3.3; adjusted with 50% Ortho-phosphoric acid) (70:30, v/v), on a Agilent Zorbax C18 column (150 × 4.6 mm i.d., 5 µm) using isocratic elution at a flow rate of 1.0 mL/min with ultraviolet detection at 266 nm. The method was validated over a concentration range of 0.1-3.0 µg/mL (r2 ≥ 0.998) with coefficient of variation for intraday precision of 3.92%, 3.56% and 2.28% for 0.5, 1.5, and 2.5 µg/mL respectively. The lower limit of detection was 50 ng/mL. The extraction recovery rates for palbociclib ranged from 66.69% to 80.97%. The intra- and inter-day precision was below 5.0%, and the...
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Posted by admin on Aug 31, 2018 in |
Atherosclerosis is a condition where the arteries become narrowed and hardened due to an excessive build up of plaque around the artery wall. The disease disrupts the flow of blood around the body, posing serious cardiovascular complications. Liver X alpha receptor (LXRα) is highly expressed in liver, function as cholesterol sensors with important roles in regulating cholesterol homeostasis. The leaves of Corchorus trilocularis possess anti-atherosclerotic property along with its anti hyperglycemic activity. The Genetic Optimization for Ligand Docking (GOLD) software resulted in identifying the best compound that interacts with the receptor. The present study was carried out to evaluate the anti-atherosclerotic activity of the ethanolic leaf extract of Corchorus trilocularis against LXRα by...
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Posted by admin on Aug 31, 2018 in |
Medicinal plants and natural products have many applications in the treatment of viral diseases, due to much lower side effects. Different types of hepatitis, especially hepatitis B, are of the viral diseases whose treatment using herbal medicines is currently of great interest due to the limited availability of effective chemical drugs and having numerous side effects. Due to the attention paid to herbal drugs and their importance for the treatment of hepatitis B, we conducted this review to take a look at the evidence regarding the action mechanisms of viruses, antiviral herbal remedies and various herbal anti-hepatitis drugs and their therapeutic mechanism published until 2017. To provide data for conducting this review, the most up-to-date electronic journals including those indexed in the Pubmed, Elsevier, Institute for Scientific Information, Google Scholar and Scientific Information Database databases and various books, has been used. There are several plants for the treatment of viral diseases, including hepatitis B, which prevent or decrease infection via various mechanisms. Medicinal plants counteract viruses by various mechanisms,...
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Posted by admin on Aug 31, 2018 in |
Bioanalysis is a sub-discipline of analytical chemistry covering the quantitative measurement of xenobiotics (drugs and their metabolites and biological molecules in unnatural locations or concentration) and biotics (macromolecules, proteins, DNA, large molecule drugs, metabolites) in biological system. The focus of bioanalysis in the pharmaceutical industry is to provide as quantitative measure of the active drug and/or its metabolite(s) for the purpose of pharmacokinetics, toxicokinetics, bioequivalence and exposure response (pharmacokinetics / pharmacodynamics studies). Over the past several years dried matrix spot (DMS) sampling technique has emerged as a pertinent method in both qualitative and quantitative bioanalysis context. There are many types of DMS techniques such as dried blood spot (DBS), dried plasma spot (DPS), dried urine spot (DUS) and dried breast milk spot (DBMS). The most commonly used technique is DBS wherein the blood sample is directly soaked on to a paper (with or without treatment) and after drying it can be analyzed by modern analytical, immunological or genomic detection systems. Several advantages of DMS techniques such as low sample...
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