Posted by admin on Aug 31, 2018 in |
The percutaneous delivery of salicylates to muscle and joints via the application of trolamine salicylate including transfersomes is the goal of this study and is beneficial for the treatment of inflammatory muscle, tendon and joint diseases. In this study, Trolamine salicylate permeability parameters through rat skin were evaluated with different trasfersome formulations in comparison with controls with Franz diffusion cells. Transfersomes were prepared with Solvent evaporation technique. Full factorial design was applied for the experimental design and data analysis. Ethanol / lipid ratio, percentage of sodium cholate, and homogenizer rate were considered as independent variables. On the other hand, transfersome size, drug loading, stability, drug release and skin permeability parameters were regarded as responses. The results showed that the main barrier for Trolamine salicylate permeability was the horny layer and partitioning from aqueous donor phase into the skin was rate limiting step for drug flux. Maximum flux and diffusion coefficient enhancement obtained by transfersomes no. 8 and 7 were 5.5 and 2.2 – folds, respectively. Regression analysis suggested significantly...
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Posted by admin on Aug 31, 2018 in |
Excessive antimicrobial use leads to adverse drug effects, thus increased healthcare costs and promoting the emergence of antimicrobial resistance worldwide. The aim of the study was to evaluate the prescription pattern of antibiotics in pediatric outpatients at private hospitals in Rajshahi city of Bangladesh. WHO / INRUD prescribing indicators were used for the analysis of prescription pattern. A total of 329 pediatric prescriptions were analyzed of which 54% male and 46% female. Most of the patients (48%) belongs to the age group of 1 year – 6 years. Body weight was not mentioned in 17% of prescr-iptions, diagnosis was confirmed only in 11% patients and also 11% of children did not take or completed immunization. Among the children born 83% were of caesarian and 17% of normal delivery. The results also indicated that 964 drugs were used by the patients with an average 2.93 per prescription. However, none of the drugs was prescribed in generic name. Interestingly, only 37.24% drugs were prescribed from the national essential drug list. Children...
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Posted by admin on Aug 31, 2018 in |
Chalcones (3a-m) was prepared by condensing 4-aminoacetophenone (1) with various aromatic aldehydes (2a-m) according to Claisen-Schmidt condensation. These 4-aminochalcones on cyclization with guanidine hydrochloride under basic alcoholic conditions gave 2,4,6-trisubstituted pyrimidines 4a-m with quantitative yields. Novel 4-amino-5,7-disubstituted pyrido[2,3-d]pyrimidines 6a-g were synthesized via a facile regioselective cyclization followed by aromatization of 2-amino-3-cyano-4,6-disubstitutedpyridines 5a-k which were in turn prepared from 4-aminochalcones 3a-m. All the synthesized compounds were characterized by spectroscopic means (FT-IR, 1H and 13C NMR, LC-MS) and elemental analysis and screened for anticancer activity by in-vitro MTT assay against Hos, HT 29, G 361, A 549 and DU 145 cell lines. Pyridopyrimidine nucleus was found to be the best pharmacophore than chalcone and pyrimidine nucleus in cancer therapy. And also it was found that compounds which possess 2,4-dichlorophenyl (3b,4b and 6b), 4-fluorophenyl (4c and 6c) and 4-dimethylamino (3i,4i and 6g) moieties as substituent at respective position in chalcones, pyrimidines and pyrido[2,3-d]pyrimidines identified as lead molecules. All the molecules were found to possess better activity against Hos (bone cancer) and G...
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Posted by admin on Aug 31, 2018 in |
The nature harbours a huge source of active principles possessing antimicrobial property. This phenomenon provokes microbiologists, ethnopharmacologist and natural product chemists to perform extensive research on phytoprinciples. The extensive use of these principles in phytotherapy is becoming an excellent alternative strategy against the occurrence of microbial resistance due to some conventional medicines. The current research article dealt with ethanolic crude extractives from the dried leaves of Ocimum sanctum Linn. and fresh Leaf stalks of Piper betle Linn. were screened for its antimicrobial activity using the agar-well diffusion method. The sample of the mentioned herbs could be easily found in large amount from the environment of north east region in India. In our present study with Ocimum sanctum L. (tulsi) leaf extract showed marked inhibitory effect on Klebsiella pneumoniae, Pseudomonas aeruginosa, E.coli, Vibrio cholerae where a highest zone of inhibition estimated was 30 mm against E. coli. Tulsi extractive possess partial inhibition against Candida albicans. Crude ethanolic extractive of Piper betle Linn. (Paan) stalk remarkably inhibited. A. niger, Escherichia coli and Candida albicans where the...
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Posted by admin on Aug 31, 2018 in |
Objective: Brucellosis in humans is the most common zoonotic infection in the world. Brucellosis can be prevented by vaccination but no effective vaccines are known for prevention of human brucellosis and minimal impact of animal brucellosis. Outer membrane proteins (OMPs) of Brucella sp. have been the focus of vaccine development and the diagnosis of brucellosis. Methods: The pathogenesis study of brucellosis used rat by intraperitoneal injection. Profile OMP detected by SDS-PAGE. The antibodies took from rabbit and the immune response did with western blotting, dot blot and immuno histochemistry. Results: B. abortus S19 cell to the rat show the presence of inflammatory cells and bleeding in the uterine rat. The 36 kDa OMP could isolated from B. abortus S19. Rabbit antibody to 36 kDa OMP can detect B. abortus of this histological of the uterus. OMP 36 kDa B. abortus S19 is antigenic characterized by its ability to stimulate antibodies in the host’s body (Oryctolagus cuniculus). Conclusion: B. abortus S19 can be detected in histopathology of rats uterus shown...
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