Posted by admin on Aug 1, 2013 in |
Drug delivery systems including chemical, physical and biological agents that can enhance the bioavailability, improve pharmacokinetics and reduce toxicities of the drugs. Carrier erythrocytes are one of the most promising biological drug delivery systems investigated in recent decades. Resealed Erythrocytes are biocompatible, biodegradable, possess long circulation half-life and can be loaded with variety of active drug substances. Resealed erythrocyte has got several advantages over the other drug delivery system which makes it superior than other systems. Carrier erythrocytes are prepared by collecting blood sample from the organism of interest and separating erythrocytes from plasma. By using various methods, the cells are broken and the drug is entrapped into the erythrocytes, finally they are resealed and the resultant carriers are then called “resealed erythrocytes”. Resealed erythrocytes, as a drug delivery system has excellent capacity to enhance the therapeutic index and patient compliance. It has got tremendous potential to achieve site specific drug delivery with least wastage of drugs and it also prolong the release of drug. So many drugs like...
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Posted by admin on Aug 1, 2013 in |
Traditional knowledge and ethno–botanical use of plants have been widely acknowledged all over the world. In the present scenario, emergence of multiple drug resistance to human pathogenic organisms, this has necessitated a search for new antimicrobial substances from other sources including plants. Combretum albidum, a traditional medicinal liana, used by rural peoples, villagers and tribals residing in different parts of Maharashtra, Tamil Nadu and Kerala, to treat various ailments. The Muthuvans, Chinnar tribe of Idukki district, Kerala is using stem bark of this plant as a remedy for Jaundice. The present study carried out to check the antimicrobial activity of water and methanol extracts of stem bark of C. albidum. The result showed significant antibacterial activity against Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, Pseudomonas aeruginosa, Bacillus subtilis and Salmonella...
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Posted by admin on Aug 1, 2013 in |
A simple HPTLC method has been developed for the estimation of Gallic acid and Piperine in methanolic extract of Zeal herbal granules [ZHG], a Polyherbal formulation. The separation was performed on TLC aluminum plates precoated with silica gel 60 F254, good separation was achieved in the mobile phase of toluene: ethyl acetate: formic acid (11: 15: 1v/v/v) and densitometry determination of Gallic acid (0.50 Rf) and Piperine (0.70 Rf) was carried out at single wavelength scanning at 254 nm. The method validated as per ICH guideline, shows regression co-efficient value for Piperine 0.9992 and 0.9979 for Gallic acid in range of 40-90 µg/mL Recovery of Piperine and Gallic acid in final product found in range of 98-102%. Precision study (interday & intraday) showed that the relative standard deviation is less than 2%, showing method is well precise. Quantification study shows 0.23 % w/w of Piperine and 0.37 % w/w of Gallic acid in ZHG. Present study shows that developed method is simple, precise, specific, robust and accurate, and could...
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Posted by admin on Aug 1, 2013 in |
The critical nature of pharmaceutical dosage forms requires that both formulation and process variables are optimized in order to achieve desired quality outcome. In this study, process optimization is examined with a view to position the final product in such a way as to deliver the expected performance. Six formulations were designed with similar excipients and nifedipine as model drug. Each formulation went through wet granulation process, during which the granulation time, amount of granulation liquid and wet milling using different sieve sizes were modified to reflect the parameters being investigated. Results vindicated the importance of modification of process variables to the extent that significant difference was observed in the physicochemical parameters, especially in vitro dissolution and drug release rates. Data from in vitro dissolution testing was fitted into different kinetic models so as to predict the order and mechanism of drug release. It could be concluded from this research work that through logical process optimization, it is possible to identify critical process parameters that can be modified to...
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Posted by admin on Aug 1, 2013 in |
Aim: To evaluate the in vitro antioxidant, antimutagenic and cytotoxic property of hydro-alcoholic extract of Thymus vulgaris L. by subjecting it to free radical scavenging, Ames and MTT assay respectively. Methods: Powdered Thyme leaves were extracted in a Soxhlet apparatus using 70% ethanol to obtain a dry extract. The extract was assessed for its total phenolic, tannin, flavonoid and flavonol content using quantitative assays. The antioxidant potential of Thyme was determined using DPPH•, ABTS•+, FTC and metal chelating assays. The in vitro inhibitory effects of Thyme on lipid peroxidation of liver and kidney along with erythrocyte hemolysis were performed following standard procedures. Further, the antimutagenic property of Thyme was investigated using Ames assay against TA 98 and TA 100 mutant strains of Salmonella typhimurium. The in vitro cytotoxicity assay of Thyme was examined following the MTT assay against Hep 2 and Hep G2 cell lines. Results: The hydro-alcoholic extract of Thyme registered 91.42 ± 0.90, 63.23 ± 1.02, 55.01 ± 0.42 and 65.69 ± 0.43 mg/g extract of total...
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