Posted by admin on Jul 1, 2014 in |
The World Health Organization (WHO) defines traditional medicine as “the health practices, approaches, knowledge and beliefs incorporating plants, animals and mineral-based medicines, spiritual therapies, manual techniques and exercises, applied singularly or in combination to treat, diagnose and prevent illness or maintain wellbeing. Various compounds have been isolated from Bridelia micrantha some of which areFriedelin, Taraxeronie, Epifiedelinol, Taraxerol, Garlic acid, Ellagic acid and sitosterol. Fresh stems of B. micrantha collected, dried, pulverized and mixed with sterile distilled water, extract left for 72hrs and stirred at 3hourly, filtered and concentrated in a water bath, stored in a refrigerator. Experiments were carried out using mice and wister rats of both sexes. The LD50 following the intraperitoneal route was estimated to be 20.3 mg/kg. The surviving animals following intraperitoneal route exhibited toxicological signs of writhing, decrease loco-motor activity, sedation and dose dependant mortality. Haematological parameters in the rat after 28days of daily oral administration show no significant difference between the treated groups and control. However, lymphocytes at the dose of 1000mg/kg indicate significant...
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Posted by admin on Jul 1, 2014 in |
A simple, precise, accurate, economical and reproducible HPLC method for estimation of Olmesartan in tablet dosage form has been developed. Quantitative HPLC was performed with HITACHI L2130 with D Elite 2000 Software with Isocratic with UV-Visible Detector (L-2400), PUMP (LC-IOAT). C18 Develosil ODS HG-5 RP 150mm x 4.6mm 5µm particle sizecolumn receptor in vascular muscle. Its action is therefore independent of the pathway of angiotensin II synthesis was used in the study.The mobile phase of ACN: phosphate buffer (pH 3.1) was used in this study. The conditions optimized were: flow rate (1.0 ml/minute), wavelength (205nm) and run time was 10 min; column temperature was maintained at 400C. Retention time was found to be 6.31 min. The linearity was found to be in the concentration range of 10-100 mg/ml. The developed method was evaluated in the assay of commercially available tablets OLMESARcontaining Olmesartan. The amount of drug in tablet was found to be 40mg. Results of analysis were validated statistically and by recovery studies. The recovery studies 99.27 % was...
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Posted by admin on Jul 1, 2014 in |
Aim: Isniazid is unstable in aqueous medium. The aim is to improve the aqueous stability of isoniazid by microencapsulation method. Methods: Isoniazid microcapsules were prepared by temperature change method. Ethyl cellulose used as coating material which forms a layer on pure drug. Ethyl cellulose was insoluble in cold cyclohexane when temperature rise about 70-750C it become soluble in cyclohexane. Result: Isoniazid was reported unstable in aqueous medium and their high acetylation occurs in liver. Microcapsules were prepared by temperature change method. The average particle size of isoniazid microcapsules was found to be 57µm and angle of repose was 23.560, flow rate 0.85gm/sec. Isoniazid in pure form released 90% about in 50 minutes. After microencapsulation T90% was increased from 50 minutes to about 4 hours. Conclusion: After microencapsulation improved the physical properties like bulk density, particle size, and flow characteristics. Isoniazid microcapsules also show prolonged the release by following first order kinetics and enhanced the stability in aqueous medium....
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Posted by admin on Jul 1, 2014 in |
Polycarpaea corymbosa has been used traditionally as medicine for the treatment of various ailments. In the present investigation an attempt has been made to quantify phenol, flavonoid and steroid in methanol extract of aerial and root parts of P.corymbosa Lam. by HPTLC method. Different compositions of the mobile phase for HPTLC analysis were tested in order to obtain high resolution and reproducible peaks. The result of HPTLC analysis authenticates the presence of phenols, flavonoids and steroid with several peaks having different Rf values. Our results revealed that at least three types of phenols were seen in aerial methanolic extract and one in root extract. Similarly five types of flavonoids and three types of steroids were found in the Polycarpaea corymbosa whole plant methanolic extract. Phenol and flavonoid were found to have higher concentration in the methanolic extract when compared to steroids. Therefore, from the above results, methanolic extract of P.corymbosa can be used as therapeutic agents to treat various disorders caused by free radical and chemical substances due to...
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Posted by admin on Jul 1, 2014 in |
The present paper deals with synthesis and characterization of some new chromium (III) Schiff base complexes using microwave irradiation technique as well as conventional heating. The S∩N donor benzothiazolines, 1-(2-furanyl) ethanone benzothiazoline (Bzt1N∩SH), 1-(2-thienyl) ethanone benzothiazoline (Bzt2N∩SH) and 1-(2-pyridyl) ethanone benzothiazoline (Bzt3N∩SH) were prepared by the condensation of ortho-aminothiophenol with respective ketones in ethanol. The chromium (III) complexes have been prepared by mixing CrCl3∙6H2O in 1:1 and 1:2 M ratios with benzothiazolines. The structure of the ligands and their transition metal complexes were confirmed by the elemental analysis, melting point and molecular weight determinations, IR, 1H NMR, electronic, EPR spectral studies. On the basis of these studies an octahedral environment around the chromium (III) ionhas been proposed. The in vitro antimicrobial and in vivo antifertility activities of Schiff base ligands and their respective chromium (III) complexes were performed on pathogenic bacterial and fungal strains and male albino rats respectively. The results indicated that the complexes showed higher activity than the parent...
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