The phytochemical screening of the bark of Albizia lebbeck (L) showed positive test for tannin, alkaloid, flavonoid, terpenoid, phlabotannin, saponin, steroid and cardiac glycoside. In this article an attempt was made for biological studies (the antimicrobial activity, cytotoxicityand free radical-scavenging activity) of different extracts (hexane, methanol, ethyl acetate, water and buta...
Betulin, a known pentacyclic tri-terpenoid, possessing diverse pharmacological properties was determined using HPLC in stem bark methanol extract of Tectona grandis Linn. distributed in greater part of India. Furthermore, Tectona grandis Linn. is used traditionally in treatment of bronchitis, dysentery, headache, leukoderma, leprosy and constipation. The present study was aimed to validate HPLC me...
COMPARATIVE PHARMACOGNOSTIC AND PHYTOCHEMICAL STUDIES OF THE RAW DRUG SOURCES OF PRASARINI USED IN AYURVEDA
According to classical texts Paederia foetida is used as the genuine source plant of the drug Prasarini, it posses properties like astringent, aphrodisiac, laxative, bitter etc. In Ayurvedic practitioners Merremia tridentata ssp. tridentata and Merremia tridentata ssp. hastata are used as the source plant of this drug. However no conclusive pharmacognostic study of these plants has been performed ...
Time dependent density functional theory (TDDFT) calculations have been carried out to study the electronic structure and the UV absorption spectra of Triamterene. The UV spectra have been investigated with inclusion of solvent effect. The B3LYP functional with a 6-31G(d, p) basis sets have been used to compute absorption energies. The solvent effects have been described within the polarizable con...
DEVELOPMENT AND VALIDATION OF SIMPLE EFFECTIVE HPLC METHOD FOR THE QUANTITATIVE DETERMINATION OF RELATED SUBSTANCE PRESENT IN SITAGLIPTIN PHOSPHATE DRUG SUBSTANCE
An efficient and sensitive RPHPLC method was developed and validated for the estimation of Sitagliptin Phosphate process impurities in drug substances which were identified and characterized by LCMS, FTIR, 1H NMR, C NMR techniques. The method was carried out on a Symmetry shield RP 18 column at 25°C using a 1 % Perchloric acid and acetonitrile in gradient mode of pump. The flow rate is 1.2 ml/min...
The most common cause of death is bone marrow failure. Chromosomal analysis is essential for the diagnosis, response to treatment and prognosis of leukemia. The present study was done to know the chromosomal abbreviation in chronic leukemia of patients with in age group of 2 to 60 years. 32 male and 21 female patients have been analyzed for this study. The chosen leukemic patients were those who w...
HIGH FREQUENCY DIRECT SOMATIC EMBRYOGENESIS FROM THE JUVENILE SHOOT BUDS OF CHLOROPHYTUM BORIVILIANUM SANT. ET FERNAND AND PRODUCTION OF ARTIFICIAL SEEDS
The study presents a protocol for mass propagation of Chlorophytum borivilianum through direct somatic embryogenesis. Direct somatic embryos occurred on the surface of juvenile shoot bud sprouts cultured on Murashige and Skoog medium supplemented with 5 mg L-1 BAP, yielding a high frequency of direct somatic embryos. On the other hand, a much lower concentration of BAP (0.5 mg L-1) led to th...
Bioanalytical method development is the process of creating a procedure to enable a compound of interest to be identified and quantified in a biological matrix. A compound can often be measured by several methods and the choice of analytical method involves many considerations. Analysis of drugs and their metabolites in a biological matrix is carried out using different extraction techniques like ...
EVALUATION OF CARDIO PROTECTIVE EFFECT OF AVERRHOA CARAMBOLA FRUIT IN ISOPROTERENOL INDUCED MYOCARDIAL INFARCTION IN RATS
Myocardial infarction develops due to ischemia induced oxidative stress. Averrhoa Carambola fruit (ACF) is reported to possess strong antioxidant activity which may protect the heart against isoproterenol (ISO) induced myocardial infarction (MI) in rats. To evaluate this activity Averrhoa carambolafruit extract (ACFE) (200mg/kg & 400mg/kg, p.o) was administered to male wistar rats (180-250g) f...
EVALUATION OF ADVERSE EFFECTS DUE TO ANTIDIABETIC AND CARDIOVASCULAR DRUGS IN A TERTIARY CARE HOSPITAL IN KOLKATA, WEST BENGAL, INDIA
Purpose: Diabetes mellitus is a significant burden to the present society, associated with various co-morbid conditions like hypertension, cardiovascular diseases, etc. A number of drugs are available to handle these health problems successfully. However, therapeutic success of such diseases is limited due to adverse effects of drugs or medicines. The aim of the present study was to evaluate the...