Posted by admin on Sep 1, 2012 in |
Background: β-blocker is an established therapeutic strategy for stage C systolic heart failure based on the American Heart Association guideline, nonetheless limited studies analyzed the role of β-blocker primarily Bisoprolol adding to ACE inhibitor and furosemide combination on the left ventricular function in systolic heart failure patients. Objectives: This study was designed to analyze the role of Bisoprolol adding to ACE inhibitor and furosemide combination on the ventricular function in patients with systolic heart failure particularly on ejection fraction and quality of life. Subjects & Methods: 40 to 80 years old ambulatory patients (N=30) at Dr. Saiful Anwar General Hospital Malang diagnosed as stage C systolic heart failure receiving optimum dose of inhibitor ACE and furosemide combination for at least 2 months. Before Bisoprolol was added, the ejection fraction and the quality of life was observed to assess the baseline left ventricular function then re-observed after 3 months. Results: After the adding of Bisoprolol, there were significant differences in ejection fraction (measured by echocardiography) and quality of life (measured...
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Posted by admin on Sep 1, 2012 in |
Natural resources in general and plant materials in particular are receiving more attention due to their safety as pharmaceutical excipients. Present work assessed the combination potential of natural hydrophobic resin, rosin, and synthetic polymer ethyl cellulose to mask the abhorrent inherent taste of ambroxol hydrochloride, by microencapsulation technique, and its possibility to formulate as a fast dissolving dosage form. Being of natural origin, rosin and its derivatives are biodegradable and biocompatible. Although It has excellent film forming property the native rosin films are brittle and break easily upon handling. The film forming properties of rosin was modified by substituting a part with ethyl cellulose.The prepared rosin-ethyl cellulose composite microspheres by emulsion solvent evaporation technique possessed good sphericity, smooth surface morphology, uniform and narrow size distribution (10-90 µm), when analyzed by scanning electron microscopy. PEG 400 was used as plasticizer because of its hydrophilicity, biocompatibility and their excellent plasticizing activity. Method of preparation has influenced the particle size and drug loading efficiency. Drug-polymer compatibility was confirmed by Fourier transform infrared...
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Posted by admin on Sep 1, 2012 in |
Eurycoma longifolia (E. longifolia) Jack has been widely used in traditional medicine for its antimalarial, aphrodisiac, anti-diabetic, antimicrobial, anti-pyretic and anticancer activities. As yet, no study has been done on biosafety of partially purified sub-fraction (TAF2) of the crude methanolic extract of E. longifolia roots. This study aims to evaluate, in vivo, the effects of TAF2 on the haematological, biochemical and histology parameters in nude mice. Male nude mice were treated orally with TAF2 (200 mg/Kg/mouse) every other day for 16 days. Untreated mice were used as controls. Blood samples were collected by intra-cardiac puncture and haematological and biochemical parameters were analyzed by using animal blood counter and automated chemistry analyzer, respectively. Hematoxylin-eosin stain (H&E) was used to evaluate the histopathology changes. Blood cells morphology was assessed by Wright stain of the thin blood films. The results showed normal blood haematology and clinical chemistry. Histological examination of TAF2 treated vital organs (kidney, liver and spleen) of mice showed no apparent change with the dose used. In conclusion, E. longifolia...
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Posted by admin on Sep 1, 2012 in |
Allium sativum L. (Liliacea) is a perennial bulb with a tall, erect flowering stem. The bulb of the plant has been used in many parts of the world as a stimulant, carminative, antiseptic, expectorant, anthelmintic and diuretic. This study has been planned to assess the diuretic activity of fresh garlic bulb extract targeting the steroidal and triterpenoidal saponin content. The rats were randomly divided into 4 groups of 5 animal each as vehicle control (2 % tragacanth suspension), standard drug frusemide (20 mg/kg, p.o), and n-butanol extract (10 mg/kg and 20 mg/kg, p.o) treated. Urine was collected in a graduated cylinder and its volume was measured for next 5 hr. Na+, K+ and Cl– concentrations were measured. Phytochemical analysis of A. sativum n-butanol fraction showed presence of steroids, triterpenoidal saponins and carbohydrates. At 20 mg/kg dose onset of diuresis and total volume of urine formed was significantly (P<0.01-0.05) higher. Fifth hour urine volume at 20 mg/kg dose was 9.3 ml as compared to 5.5 ml of control. Extract at...
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Posted by admin on Sep 1, 2012 in |
A simple, sensitive and reproducible method was developed and validated for the simultaneous estimation of Diethylcarbamazine and Levocetirizine in its tablet formulation by reverse phase high performance liquid chromatography using Waters1515 HPLC with Diethylcarbamazine and Levocetirizine simultaneously. HPLC-PDA detector at the λmax of 253nm, using Hypersil-BDS C18 (250×4.6 mm. 5 μ) column. The mobile phase used was potassium dihydrogen orthophosphate buffer (pH: 5): acetonitrile (20:80 v/v) with isocratic flow (flow rate 1 ml/min) and the pH was adjusted with orthophosphoric acid. The compounds Diethylcarbamazine, Levocetirizine were eluted at 2.04, 5.54 min, respectively. The peaks were eluted with better resolution. The method was accurate with assay values of 99.67% and 99.81% w/w, precise (%RSD) with 0.3and 0.5, percentage recovery values of pure drug were in between 99.4% to 100% and 99% to 99.4% which are very sensitive with limit of detections (LOD)’s 2.42 and 0.08ppm and limit of quantification (LOQ)’s 7.42 and 0.2ppm, these results are within the range of limits 98% to 101% which indicates that the method was...
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