Posted by admin on Feb 1, 2012 in |
A simple, accurate, precise and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method for the determination of cefixime trihydrate and its degraded products have been developed and validated. Drug was resolved on a C18 column (waters spherisorb 25 cm × 4.6 mm, 5µm), utilizing mobile phase of sodium dihydrogen phosphate monohydrate (0.1M aqueous) pH adjusted to 2.5 with diluted orthophospharic acid (10 % aqueous) and methanol in a ratio of 3:1 respectively. Mobile phase was delivered at the flow rate of 1.0 ml/min. Ultra violet detection was carried out at 254 nm. Separation was completed within 9.75 minutes. Calibration curve was linear with correlation coefficient (r2) = 0.9996 over a concentration range 10-50µg/ml. Recovery was between 98.84, 100.25 percentage. Method was found to be reproducible with relative standard deviation (RSD) for intra and interday precision of < 1.0 over the said concentration range. The method was successfully applied to the determination of the decomposed products of cefixime trihydrate, it can be very useful and an alternate to performing...
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Posted by admin on Feb 1, 2012 in |
Purpose: The present study was designed to evaluate the phytochemical, biochemical and antimicrobial activity of leaf extracts of Boerhavia erecta and Chromolaena odorata. Methods: The leaves of Boerhavia erecta and Chromolaena odorata were washed with tap water followed by distilled water. The cleaned leaves were shade dried and then made to fine powder by using homogenizer. About 1.0 gram of clean dried leaf was taken along with 10 ml ethanol and mashed well in a homogenizer and filtered, then used for biochemical,phytochemical analysis and antimicrobial activity by using various standard methods. Results and Conclusions: Since, these plants possess many medicinal properties and the results showed that both the plant extracts possessed significant levels of minerals and vitamins. The presence of biochemical constituents and positive antimicrobial activity expertise its medicinal property. Natural products perform various functions, and many of them have interesting and useful biological activities. Boerhavia erecta and Chromolaena odorata are plants which are extensively used in folk medicine....
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Posted by admin on Feb 1, 2012 in |
The objective of the study is to evaluate the individual and combined effects of hydroxy propyl β cyclodextrin (HPβCD), surfactant (Poloxamer 407) on the solubility and dissolution rate of nimesulide, a BCS class II drug in a series of 22 factorial experiments. The solubility of nimesulide in four selected fluids containing HPβCD and Poloxamer 407 as per a 22 factorial study was determined. HPβCD alone gave a marginal increase (1.47 fold) in the solubility of nimesulide. Whereas Poloxamer 407 alone and combination of HPβCD and Poloxamer 407 gave a significantly higher enhancement in the solubility of nimesulide, 2.5 and 6.52 fold respectively. Solid inclusion complexes of nimesulide- HPβCD were prepared with and without Poloxamer 407 by kneading method as per a 22-factorial design. Both the individual and combined effects of HPβCD and Poloxamer 407 on the solubility, dissolution rate and dissolution efficiency (DE30) of nimesulide were highly significant (P < 0.01). HPβCD and Poloxamer 407 alone gave 13.17 and 5.20 fold increase in the dissolution rate of nimesulide. Combination...
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Posted by admin on Feb 1, 2012 in |
Aceclofenac, a non-steroidal anti- inflammatory drug, with poor solubility and bioavailability was taken as candidate for enhancement of in vitro dissolution and in vivo bioavailability. Development of Aceclofenac orally disintegrating tablet (ODT) using lyophilization technique was adopted. The ODTs were prepared by freeze-drying an aqueous dispersion of Aceclofenac, matrix former, filler (sugar alcohol), and an anti-collapse. The tablets were evaluated compendial (uniformity of weight, uniformity of content, friability, in vitro disintegration time and in vitro dissolution), together with wetting time, in vivo disintegration time, moisture analysis and scanning electron microscopy. The compendial results showed that lyophilized ODTs disintegrated within few seconds and showed significantly faster dissolution rate of Aceclofenac in comparison with commercially available immediate release tablet Aceclofenac tablet (Bristaflam®). In vivo evaluation for the best chosen Aceclofenac ODT formulation (LA#10) was done for determination of the drug pharmacokinetics in comparison with the immediate release tablet Aceclofenac tablet (Bristaflam® 100 mg). A randomized crossover design was adopted in the comparative bioavailability study done on four healthy volunteers. Statistical analysis...
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Posted by admin on Feb 1, 2012 in |
Atherosclerosis involves disturbances in endothelium and the resultant cascade of inflammatory reactions involving generation of ROS (Reactive Oxygen Species). Peroxisome proliferator-activated receptor-g (PPAR-g) receptors are more expressed during atherosclerosis process and its stimulation results in inhibition of formation of atherosclerotic plaque. N-acetylcysteine (NAC) stimulates GSH (glutathione) formation and thus helps in scavenging ROS. Thus, the purpose of present study is to explore the role of PPAR-g agonist (pioglitazone) and N-acetylcysteine in atherosclerosis. Endothelial injury in hyper-lipidemic rat was produced in the femoral artery. The effect of chronic treatment of pioglitazone, N-acetylcysteine and their combination was evaluated by measuring serum HDL, LDL, triglyceride, total cholesterol and lesion index. Pioglitazone and N-acetylcysteine treated rats showed higher levels of HDL and lower levels of LDL, triglycerides and total cholesterol as compared to model control animals (diet + endothelial injury model). Combination of both pioglitazone and N-acetylcysteine produced synergistic action with respect to lipid level. N-acetylcysteine treated animals showed high GSH levels in the liver. Femoral artery lesion index was significantly less in...
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