Posted by admin on May 31, 2022 in |
Hypothyroidism is a progressive disorder characterized by varying degrees of thyroid failure, metabolic consequences, and hemodynamic changes that decrease renal plasma flow and glomerular filtration rate, increasing serum creatinine level. Therefore, the present study aimed to evaluate and analyse the level of serum urea, creatinine and thyroid function tests in cases of subclinical hypothyroidism and apparently healthy controls and determine whether thyroid dysfunction has detrimental effects on serum creatinine and urea levels in subclinical hypothyroidism cases. Both thyroid function tests (TSH, T3, and T4), serum urea, and creatinine were analyzed in 45 cases with subclinical hypothyroidism and 45 healthy subjects. Patients with subclinical hypothyroidism had a statistically significant rise in creatinine levels compared to controls. In contrast, there was no significant difference in serum urea levels between patients and controls. There was likewise a positive correlation between creatinine and urea in patients with subclinical hypothyroidism. The study results indicate that renal function should be regularly monitored in patients with subclinical...
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Posted by admin on May 31, 2022 in |
Punica granatum (Pomegranate) external peel extract in 70% ethanol was challenged against two non-albicans Candida spp. to find out it’s possible antifungal effects in disc diffusion technique as well as in microtiter dilution to find out MIC levels. Although both showed sensitivity zones in disc diffusion technique at 100 mg/mL concentration of the extract, at lower concentrations (<16.6 mg/mL), only C. guilliermondii showed MIC value at 0.52 mg/mL, while C. tropicalis did not show any inhibition of growth at these concentrations of the...
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Posted by admin on May 31, 2022 in |
The present study aims to develop a verse phase HPLC method for the simultaneous estimation of Metformin and Saxagliptin. In bulk and pharmaceutical dosage form and to validate the proposed method in accordance with ICH guidelines for the intended analytical application. Multiple trials were run to Metformin and Saxagliptin, get eluted with good peak symmetric properties. Mobile phase Phosphate buffer: Methanol: Acetonitrile (40:5:55), Column Hypersil silica and flow rate 1.0ml, detection wavelength at 233nm, column temperature 300C and diluents Water: Methanol (50:50). Column Hypersil silica and flow rate 1.0 ml, detection wavelength at 210 nm conditions were finalized as an optimized method. This method was validated for Stability Data, System suitability, Precision, Linearity, Accuracy, Robustness, Ruggedness, LOD, and LOQ. System suitability parameters were studied by injecting the standard five times, and results were well under the acceptance criteria. Linearity study was carried out between 25% to 150% levels, r2 value found 0.999. By using the above method, assay of the marketed formulation was carried out 99.63% was present. So,...
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Posted by admin on May 31, 2022 in |
A novel series 3(a–d) 1-(4-benzhydryl piperazin-1-yl)-3-(di substituted) propan-1-one &1-(4-benzhydrylpiperazin-1-yl)-3-(piperidin-1-yl) propan-1-one (3e) & 1-(4-benzhydrylpiperazin-1-yl)-3-morpholinopropan-1-one (3f) compounds have been synthesized and screened for its anticonvulsant and neurotoxic activity, After i.p. injection to rat at doses of 30, 100, and 300 mg/kg body weight. Synthesized compounds were examined in the maximal electroshock-induced seizures (MES). Spectroscopic data were consistent with the structure of newly synthesized compounds. The neurotoxicity was assessed using the rotarod method. In the prepared series, 3f was found to be active in the MES screen at 0.5 h, and 4...
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Posted by admin on May 31, 2022 in |
Leucas aspera species, auspicious in many cultures worldwide, are sources of novel bioactive secondary metabolites. The mechanism by which they exhibit therapeutic potential via antioxidant, antimicrobial and apoptotic properties is scant. The main objective of the current study was to evaluate the properties of activity-guided fractionated root methanol extract. As the methanol extract exhibited the highest antioxidant activity, it was evaluated for secondary metabolites and therapeutic properties UPLC-MS analysis of the extract identified the 15 and five secondary metabolites including oleanolic acid, 6-C-glucosyl- 8- C- arabinosylapigenin, 6- C-arabinosyl 8- C-glycosylapigenin, neohesperidin and beta-sitosterol were reported. Anti-bacterial activity by disc diffusion inhibited human pathogenic bacteria. Bacterial bio-film inhibition capacity of extract (750 µg) imaged by Confocal laser scanning microscopy revealed loss of microcolonies. Further, it quenched free radicals produced by Fentons’ reagent studied by DNS-nicking assay, indicating its strong antioxidant property. The MTT assay results identified maximum cell death for human breast cancer cells, MCF-7 (EC50, 60.31±2.71 mg/mL-1). CLSM visualized a clear indication of apoptosis via chromatin condensation. The presence...
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