Posted by admin on Mar 31, 2026 in |
The present study aimed to identify potential anti-HIV phytoconstituents from Ocimum tenuiflorum through a novel extraction strategy combined with LC–MS/QTOF-based phytochemical profiling, ADMET and PASS server predictions, HIV target proteins to elucidate bioactive compounds with promising antiviral potential. The LC-MS analysis of the extract revealed the presence of 17 compounds, including flavanones, isoflavones, phenolics, terpenoids, coumarins, and thiazole derivatives. ADMET analysis indicated that most of these phytoconstituents exhibited favourable drug-likeness properties and belonged to toxicity classes IV, V, or VI, suggesting that they are non-toxic and potentially safe for therapeutic use. PASS server predictions revealed that the compounds possess significant HIV integrase-inhibitory...
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Posted by admin on Mar 31, 2026 in |
Due of their ability to damage DNA, genotoxic impurities (GTIs) in active pharmaceutical substances need strict analytical control. Trace process-related GTIs may be present in trazodone hydrochloride, an antidepressant made using multi-step synthetic processes. Nevertheless, there is currently no approved analytical technique for the simultaneous determination of all significant GTIs related to its production. In order to quantify four process-related GTIs in trazodone hydrochloride at sub-ppb levels, a quick and selective UFLC-MS/MS approach was developed and validated. Acetonitrile-5 mM ammonium acetate buffer (pH 5.0, 70:30 v/v) was used for chromatographic separation on an Avantor ACE C18-PFP column (3 µm, 4.6 × 150 mm) under isocratic conditions. Multiple reaction monitoring was used for detection in positive ESI mode. The approach was validated over 0.060-1.200 ppb. For every impurity, linearity was attained with correlation coefficients (R²) ≥ 0.99. Across three levels, accuracy studies produced recoveries ranging from 79 to 102%. Robustness was verified with intentional changes in flow rate and column temperature, and precision satisfied acceptance standards with %RSD < 15%....
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Posted by admin on Mar 31, 2026 in |
Diabetes mellitus (DM) is a chronic and major endocrine disorder caused by an inherited and/or acquired deficiency in the production of insulin by the pancreas, or by the ineffectiveness of the insulin produced. It is a growing health problem in most countries, and its incidence is considered to be high (4%-5%) all over the world. In the present scenario, the demand for herbal products is growing exponentially throughout the world, and major pharmaceutical companies are currently conducting extensive research on plant materials for their potential medicinal value. This study focuses on the in-vitro investigation of the antioxidant and in-vivo antidiabetic activity of the Methanol and aqueous extracts of Saraca asoca (Roxb.). De Wild seeds in healthy adult Wistar albino rats weighing 200-250 g and Swiss albino male mice (25-35 g). Swiss albino mice were made diabetic by a single dose of streptozotocin (150 mg/kg i.p.). Blood glucose levels and body weights of mice were measured using weekly intervals, i.e, day 0, 7, 14, and 21 after daily administration of...
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Posted by admin on Mar 31, 2026 in |
The nephroprotective activity of the methanolic extract of Coccinia grandis (MECG) leaves was evaluated against acetaminophen-induced nephrotoxicity in Wistar albino rats. Wistar albino rats (150–200 g) were divided into six groups, and nephrotoxicity was induced using acetaminophen (750 mg/kg) administered on alternate days for 10 days. MECG was administered at doses of 125, 250, and 500 mg/kg, while N-acetylcysteine (140 mg/kg) served as the reference standard. Biochemical parameters including serum urea, creatinine, uric acid and antioxidant activity were assessed, along with histopathological examination. All treatment groups showed significant improvement in urine volume and body weight compared to the toxic control. The MECG-500 mg/kg group demonstrated the most pronounced effect (p < 0.001), restoring urine volume to approximately 9.70 mL, close to the normal baseline of 10.85 mL, while the reference group reached 10.81 mL. Lower MECG doses produced dose-dependent improvements. Histopathological analysis further confirmed reduced kidney damage in MECG-treated groups. Overall, MECG, particularly at 500 mg/kg, exhibited strong nephroprotective activity against acetaminophen-induced kidney...
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Posted by admin on Mar 31, 2026 in |
Background: Self-medication is the practice of using drugs without the guidance of a trained health care professional. With the increase in antimicrobial resistance among the masses, this study aims to find the prevalence & behavioral pattern of the practice of self-medication among undergraduate medical students. Materials & Methods: A descriptive cross-sectional study was carried out among 427 MBBS students. The questionnaire was distributed among students via a Google link that was created for this purpose. Result: Our study found that the prevalence of self- medication practice was 74.5%, of whom 71.4% practice self-medication rarely, i.e., once or twice in a year. Practice of self-medication was found to be higher among the female (77.6%) students as compared to the male (71.6%) students. Practice of self- medication was found to be higher among hostelites (76%) when compared to the day scholars (69.8%). 84.2% of the 3rd year MBBS students were found to practice self-medication, which was found to be the highest when compared with students belonging to rest of the academic...
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