Posted by admin on Mar 31, 2026 in |
The present study investigates the GC–MS profiling of stem, root, and in-vitro–derived callus cultures of Caralluma stalagmifera C.E.C. Fisch., an Apocynaceae succulent endemic to Southern India, commonly known as the “dark purple Caralluma,” and traditionally valued for its anti-obesity, antimicrobial, antidiabetic, antioxidant, and anti-inflammatory properties. The major bioactive constituents reported from the genus are pregnane glycosides and aglycone steroids. Methanolic extracts of stem and root from field-grown plants and in-vitro callus cultures were prepared and each extract was analysed using GC–MS to identify its phytochemical constituents. Compounds were identified based on retention times and mass spectral data, and comparative profiling was performed to assess variations in chemical composition among the stem, root and in-vitro callus culture extracts. The stem extract exhibited the highest phytochemical diversity with 27 compounds, including 10 major constituents. The root extract contained 17 compounds, of which 6 were major bioactive molecules. The callus extract comprised 20 compounds, including 10 major constituents, demonstrating its ability to synthesize diverse secondary metabolites under controlled in-vitro conditions. Overall, the...
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Posted by admin on Mar 31, 2026 in |
Objective: Curcuma longa and Boswellia serrata are traditional medicinal plants that have antiarthritic activity. The present research is intended to formulate a novel antiarthritic topical gel from the extract of Curcuma longa and Boswellia serrata which will increase patient compliance and stability as compared to the conventional dosage form. Material and Method: In this investigation, we created an antiarthritic herbal topical gel by combining carbopol 934 and other excipients with the methanolic extracts of Curcuma longa and Boswellia serrata in varying concentrations. For compatibility study, FTIR study would be done. Evaluation of antiarthritic gel performed by using different tests likes, pH, viscosity, spreadability, extrudability, stability, inhibition of albumin denaturation and in-vitro drug diffusion study. Result and Discussion: As a result, the formulation’s physiochemical characteristics, in-vitro antiarthritic efficacy, and stability analysis (stable even after 30 days) were assessed. Every herbal gel formulation with a pH of 5–7 had favorable outcomes for physiochemical measures. Out of all the formulations, batch no 2 exhibited superior release characteristics (79.8%) in comparison to the...
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Posted by admin on Feb 28, 2026 in |
Lower Respiratory Tract Infections (LRTIs) remain a major cause of morbidity and mortality, especially in rural healthcare settings where diagnostic limitations influence antibiotic prescribing behaviour. Aim: To evaluate the prescription pattern and antibiotic utilization among patients diagnosed with Lower Respiratory Tract Infections (LRTIs) in a rural hospital setting and to assess the rationality of antibiotic use based on WHO and ICMR guidelines. Objective: To identify the types of antibiotics prescribed, determine the proportion of broad- and narrow-spectrum agents, and assess adherence to rational prescribing practices in a government-managed rural hospital. Methodology: A prospective observational study was conducted at a rural hospital in Latur, Maharashtra, from August 2024 to March 2025. Fifty patients clinically diagnosed with LRTIs were enrolled. Data were collected using a structured form and analysed using WHO prescribing indicators to evaluate antibiotic utilization patterns and supporting medications. Results: Out of 50 prescriptions analysed, 81% contained broad-spectrum antibiotics, while only 19% contained narrow-spectrum agents. Cephalosporins and β-lactam/β-lactamase inhibitor combinations were most frequently used. Supportive drugs such as pantoprazole,...
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Posted by admin on Feb 28, 2026 in |
The current research was conducted to compare the acute oral toxicity, analgesic and anti-inflammatory properties of ethanolic extracts of Phytanthus fraternus and Impatiens balsamina, two medicinal plants that have been used traditionally in treating pain and inflammation. The acute toxicity was evaluated in Swiss albino mouse based on the OECD guideline 423 at a dose of 300 mg and 2000 mg/kg. There were no mortalities, abnormal behaviour or adverse clinical effects, which categorised the two extracts as the Globally Harmonised System (GHS) Category 5. To assess analgesic activity, the acetic acid-induced writhing test was used on mice, whereas anti-inflammatory potential has been assessed using both in-vitro and in-vivo models: the human red blood cell (HRBC) membrane stabilisation assay and carrageenan-induced paw oedema in rats, respectively. Findings revealed that both extracts had marked, dose-dependent analgesic and inflammatory effects, with the extract of P. fraternus having a uniformly higher potency than I. balsamina. Their stabilising behaviour against hypotonicity-induced haemolysis, an indicator of membrane-stabilising and anti-inflammatory activities, was confirmed with the HRBC...
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Posted by admin on Feb 28, 2026 in |
Background: Posaconazole is a potent triazole antifungal with poor aqueous solubility, while Aloin from Aloe vera exhibits complementary antifungal and wound-healing properties. Co-delivery in a liposomal gel may enhance solubility, stability, and skin penetration. Objective: To design and evaluate a novel liposomal vesicle system co-encapsulating Posaconazole and Aloin for topical antifungal therapy. Methods: Liposomes were prepared by thin-film hydration using varying ratios of soya lecithin and cholesterol, followed by incorporation into a carbopol-based gel. Formulations were characterized for particle size, zeta potential, entrapment efficiency, viscosity, pH, spreadability, in-vitro drug release, and antifungal efficacy against Candida albicans. Results: The optimized formulation (LDDS3) showed the smallest particle size (68.07 nm), highest zeta potential (−15.8 mV), and entrapment efficiency (93.22%). The gel exhibited suitable viscosity (4836 ± 0.26 cps), pH (5.6) and good spreadability. The in-vitro release followed Higuchi kinetics (R² = 0.9701), indicating diffusion-controlled release. The liposomal gel displayed a concentration-dependent inhibition zone (up to 15 mm) against Candida albicans. Conclusion: Co-encapsulation of Posaconazole and Aloin in liposomal gel significantly enhanced...
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