Posted by admin on Jul 31, 2025 in |
The aim of this study is to assess the antibacterial efficacy of the Datura stramonium seeds and leaves extracts against Mycobacterium kansasii. The antimicrobial experiments were performed using five doses (0.25µg/ml, 0.50µg/ml, 1µg/ml, 2µg/ml, 4µg/ml) of seeds and leaves extracts. The results showed notable inhibitory effects on Mycobacterium kansasii. The results indicated that the extract of D. stramonium exhibited action against all the tested bacterial isolates, with the level of activity varying depending upon the concentration. The hexane extract of seeds DSS-1H exhibited antibacterial activity, with mean zones of inhibition measuring 98.94±50.25.%, 120.98±56.92%, 120.499±69.60%, 125.752±133.18% and 89.494±180.63%, at the doses of 0.25, 0.50, 1, 2, and 4 µg/ml and while treating with DSS-2A the percent cell viability of the microbial cell was found to be 128.02±25.64%, 86.79±14.95%, 34.61±16.33%, 26.29±29.76% and 32.56±19.48% at the doses of 0.25, 0.50, 1, 2 and 4µg/ml, respectively, with incubation period of 48 hours. Similarly, the microbial cells were treated (0.25, 0.50,1,2,4 µg/ml) with DSS-3ET with incubation period of 48 hours, indicating a more substantial...
Read More
Posted by admin on Jul 31, 2025 in |
A sensitive, specific and precise HPTLC method for simultaneous estimation of Fenofibrate and Rosuvastatin has been developed and validated. The method employed aluminium plates pre-coated with silica gel 60 F254 as the stationary phase. The solvent system consisted of n-Hexane: Acetone: Ethyl acetate: Glacial acetic acid (4: 5: 0.9: 0.1, v/v/v/v) which was found to give compact and dense spots for Fenofibrate and Rosuvastatin (RF value 0.83 ± 0.05 and 0.35 ± 0.05 respectively). Densitometric analysis of both drugs was carried out at 300 nm. Linear relationship was found with calibration curve for linearity in concentration range of 25-75 mg/spot and 167-501 mg/spot for Rosuvastatin and Fenofibrate with Correlation coefficient value 0.992 and 0.991 respectively. This method was found to be good percentage recovery for Rosuvastatin and Fenofibrate in between 98% to 102%. All parameters of validation of the formulations were found within the limit as per ICH...
Read More
Posted by admin on Jul 31, 2025 in |
Patients with diagnosed BPH, suffer very frequently from lower urinary tract symptoms (LUTS). It is very crucial to find the types, prevalence, and impact of LUTS in association with age, glycaemic index, and modifiable risk factors for developing targeted management strategies. The research seeks to determine types of LUTS present in BPH patients across various age groups and their impact on QoL; and impact of glycaemic levels, as well as the sociodemographic factors like smoking and alcohol use on the severity of the LUTS/BPH. This is a prospective observational study conducted from September 2023 to February 2024 at Narayana Medical College and Hospital of Nellore district, Andhra Pradesh. 200 male patients above 45 years old with LUTS/BPH were selected by the random sampling technique. Data was collected using an IPSS questionnaire, and therefore analysed using qualitative and quantitative approaches, as well as multiple regression analysis and ANOVA. This study captures the multifactorial impacts of LUTS in BPH patients and underscores the importance of addressing modifiable risk factors and co-existing...
Read More
Posted by admin on Jul 31, 2025 in |
Kinetics of oxidation of L-cysteine and DL- Methionine by potassium permanganate in alkaline medium was studied spectro-photometrically at room temperatue. The reaction was found to be of first order with respect to amino acid, zero order with respect to oxidant and fractional order dependence on Alkali. It was found that the rate of the reaction increases with increase in concentration of [OH-]. There is a good agreement between observed and calculated rate constant under experimental conditions. The activation parameters are computed with respect to the slow step of the reaction. Cysteine exhibits relatively consistent kinetic behavior than Methionine. The oxidation product of the reaction was found to be cysteine and Methionine sulfoxide. A plausible reaction mechanism has been proposed for the experimental...
Read More
Posted by admin on Jun 30, 2025 in |
Background: Cognitive impairment is a serious problem, especially for the elderly. Cognitive impairment is often associated with neurological disorders and congenital diseases. The impact of central nervous system depressants (CNSDs) on cognition needs to be further investigated. Objective: This study aims to evaluate the relationship between CNSD use and cognitive impairment with neurological disorders and comorbidities, along with the severity of cognitive dysfunction. Materials and Methods: A prospective cross-sectional study was conducted over a 6-month period in a tertiary care hospital, including 129 patients aged 18 years and older. Cognitive function was assessed using a structured questionnaire and severity scales. Data on demographics, neurological disorders, medications, and comorbidities were collected from patient records and statistically analyzed. Results: Cognitive decline increased with age, particularly in patients aged 61+ years. Benzodiazepines were significantly associated with mild cognitive impairment. (22%, p = 0.02), while anti-convulsant and anti-Parkinsonian drugs had minimal impact. Stroke patients with comorbidities showed the highest rates of cognitive decline (25.3%, p = 0.01). Conclusion: The Results indicate that while...
Read More
