Posted by admin on Jan 1, 2011 in |
A rapid and simple high performance thin layer chromatography (HPTLC) method with densitometry at 230 nm was developed and validated for simultaneous determination of Naproxen sodium and Sumatriptan succinate from pharmaceutical preparation. Separation was performed on aluminum-backed silica gel 60F254 HPTLC plates as stationary phase and using a mobile phase comprising of methanol:distilled water:formic acid in the volume ratio of 0.5:7.5:0.1 (v/v/v), respectively. After development, plates were observed under UV light. The detector response was linear in the range of 200-1200 ng/spot and 100-1000 ng/spot for Naproxen sodium and Sumatriptan succinate. The validated lowest limit of detection was 85 ng/spot and 40 ng/spot whereas lowest limit of quantification was 200 ng/spot and 100 ng/spot for Naproxen sodium and Sumatriptan succinate, respectively. The percentage assay of Naproxen sodium and Sumatriptan succinate was found between 99.25 and 98.03 % respectively. The described method has the advantage of being rapid and easy. Hence it can be applied for routine quality control analysis of Naproxen sodium and Sumatriptan succinate from pharmaceutical preparation and...
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Posted by admin on Jan 1, 2011 in |
Meloxicam (MEL) is an oxicam derivative and a member of the enolic acid group of non-steroidal anti-inflammatory drugs (NSAIDs). Most of the reported methods for HPLC analysis of Meloxicam are cumbersome, time-consuming and expensive. Reverse phase chromatographic analysis was performed on a C18 Hi Q Sil column with methanol–water- orthophosphoric acid (80:19.9:0.1 % v/v) at a flow rate of 1ml/min and detection wavelength of 360 nm. System suitability tests essential for the assurance of quality performance of the method were performed. The method was validated for accuracy, precision, reproducibility, specificity and robustness, limit of detection (LOD) and limit of quantification (LOQ). A single sharp peak was obtained for MEL at Rt of 4.38 ± 0.02 min. The polynomial regression data for the calibration plots exhibited linear relationship (r = 0.999) over a concentration range of 4–20µg/ml and the linear regression equation was y = 43754x – 2094. Accuracy ranged from 99.33 to 100.45% and the % coefficient of variation (CV) for both intra-day and inter-day precision was less than...
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Posted by admin on Jan 1, 2011 in |
Lornoxicam (chlortenoxicam) is a non steroidal anti- inflammatory drug (NSAID) of the oxicam class with analgesic, anti-inflammatory and antipyretic properties. It is available in oral and parental dosage formulation. Lornoxicam is a Yellow or slightly yellow powder. It is slightly soluble in water, soluble in hydrochloric acid, slightly soluble in methanol. A simple spectrophotometric method was developed for the determination of lornoxicam in pharmaceutical tablet dosage form. Lornoxicam exhibiting λ max at 380 nm in mobile phase (phosphate buffer: methanol) in ratio of 50:50 and obeyed linearity in the concentration range of 1-20mcg. The proposed method was statistically...
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Posted by admin on Jan 1, 2011 in |
In the present study, eight organic solvents and aqueous extracts of Canavalia mollis leaves were screened for their phytochemical composition, anti bacterial and free radical scavenging activities. Among the different extracts tested, ethanol, methanol and acetone extracts showed significant antimicrobial and radical scavenging activities. The most susceptible microorganisms were found to be Escherichia coli, Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, and Streptococcus faecalis. Preliminary phytochemical analysis of the leaf extracts revealed that the antibacterial and the radical scavenging activities are mainly due to the presence of the phenolic compounds especially alkaloids. The results obtained suggest that C. mollis could be exploited in the infections management of various...
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Posted by admin on Jan 1, 2011 in |
Capparis grandiflora was used to treat diarrhoea and other infective disorders in folklore. The present study aims at evaluating the action of chloroform soluble fraction, ethanol soluble fraction and ether soluble fraction of the leaves of Capparis grandiflora against inflammatory diseases. The anti-inflammatory effects were investigated by employing acute inflammatory model i.e.; carrageenan-induced hind paw oedema and formaldehyde-induced hind paw oedema in rats. The ethanol soluble fraction was found to be the most potent among them which shows good anti-inflammatory response with reference to the standard drug...
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