Posted by admin on Dec 31, 2019 in |
The vast investigations on the derivatives of 1, 2, 3-benzotriazole explore wide applicability for tagging and delivering a number of other heterocyclic nuclei with benzotriazole. In the present work, several derivatives of 1-(substituted)-5- [(N-benzotriazolo-methyl)-1, 3, 4-thiadiazolyl]- imidazole-2-thione have been synthesized and are evaluated for their anticonvulsant activity. The anticonvulsant activities of the synthesized derivatives were assessed by the maximal electroshock (MES) method. Electric shock (50 mA for 0.2 sec) was delivered with the help of a corneal electrode to induce hind limb tonic extension. The synthesized derivatives X2, X3 and X6 exhibited significant anticonvulsant effect against MES-induced seizure model as an indication for compounds which are effective in grand mal...
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Posted by admin on Dec 31, 2019 in |
Bacteria predominantly remain in a self-produced polymeric matrix, adherent to an inert or living surface. This microenvironment community of bacteria is known as biofilm. Commonly visualized as a slimy layer, a number of unique features distinguish biofilms from their planktonic counterparts. Formation of biofilms depends on the extracellular signals, mechanical, biochemical, environmental conditions and genetic factors in bacteria. The attachment of biofilms is an intriguing process which is regulated by several characteristics such as substratum, cell surface, and growth medium. These biofilms cause various infections and are of at most importance when concerned about public health. One of the challenges faced during antibacterial drug development is to create compounds to counter-attack the biofilm infections. In this study, antibiofilm activities of five essential oils (EUGENOL; CINNAMALDEHYDE; PIPERIDINE; LIMONENE) were discussed. This review consisting of explicit evidence proved that plants are indeed a wonderful source to provide naturally occurring compounds for development of therapeutic and preventive agents against infections caused by...
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Posted by admin on Dec 31, 2019 in |
A bird is often subjected to dysbacteriosis with a low quality of feed. As a result, live weight gain and livability are reduced in broilers. This problem has become particularly acute now when most countries have abandoned the use of feed antibiotics. Significant assistance in this situation is provided by new regulators of intestinal biosynthesis – probiotics. In our research, we used biosporin produced by the Military and Technical Problems Centre of the Research Institute of Microbiology of the Russian Ministry of Defense. Scientific and household experience was conducted on the basis of ZAO “Ural broiler”. Three groups were formed: one control group and two experimental ones. There were 100 units in each group. The results of scientific and household experience lead to the conclusion that biostim is more preferable with the comparative use of two liquid probiotic products from the point of view of production figures. Feeding it to a bird in accordance with the instruction allows increasing the live weight of broiler chickens by 11.5%, reducing the...
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Posted by admin on Dec 31, 2019 in |
A series of hydrazide-hydrazones (3a-o) have been synthesized by the reaction of acid hydrazide (2) which is obtained from 4-carbomethoxy-2-aminophenol with aromatic acid through multi-steps. The bioactivities of the final compounds were tested with MES and scPTZ methods. The CNS toxicity was studied by the rotarod experiment. Based on the results, compounds 3d and 3o were found to be most active at 30 mgkg-1 in the MES test with prolonged duration of action; they exhibited activity comparable to standard drugs phenytoin and carbamazepine. Compounds 3f, 3j and 3m exposed toxicity (300 mgkg-1) at 0.5 h of the time period and compounds 3i, 3n and 3o exhibited late toxicity after 4.0 h as compared to carbamazepine. The rest of the compounds did not face toxicity at a maximum dose level (300 mgkg-1). This study shows that increased lipophilicity is directly related to the anticonvulsant activity. Further studies need to be carried out on other seizure tests and models of epilepsy to ascertain the precise mechanism of action of these...
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Posted by admin on Dec 31, 2019 in |
This work proposes a precise, accurate, sensitive and selective stability indicating RP-HPLC method for the simultaneous quantification of amoxicillin and enrofloxacin in bulk powder and oral suspension formulations. Chromatographic separation was performed on the reverse phase C18 analytical column using 0.1M potassium dihydrogen orthophosphate–methanol (65:35, v/v) as mobile phase and with detection at 235 nm. The retention times of amoxicillin and enrofloxacin were 3.364 min and 6.604 min, respectively. The linearity ranges were found to be 38.5-115.5-10 µg/ml (for amoxicillin) and 17.5-52.50 µg/ml (for enrofloxacin). The developed method was validated following International Conference on Harmonization guidelines. All validation parameters are fulfilled by the proposed method. Amoxicillin and enrofloxacin was subjected to different stress conditions like acid, alkali, oxidation, thermal, photo, and hydrolytic degradation. Both the analytes undergo degradation in all stress conditions. Since, the method effectively separated the analytes from their degradation products, it can be used as a stability-indicating...
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