Posted by admin on Dec 31, 2017 in |
Phenolic extracts of crude oil and methanol extracts of defatted seed cake were used to evaluate total phenolics, flavonoids content and antioxidant activity for two locations. The seeds were evaluated for their proximate composition while oils were subjected to chemical analysis. Salvadora persica had highest phenolics ranged from 12.3 ± 0.3 to 22.3 ± 0.3mg GAE/g and flavonoids content 3.7 ± 0.6 to 6.2 ± 0.2mg CAE/g. The antioxidant activity having IC50 value ranged from 0.024 ± 0.0 to 0.045mg/ml. The highest oil content as 42.5 ± 0.4% and highest protein content was 23.3 ± 0.3%. The oil content of the seeds recorded the highest iodine value 112.0 ± 0.8g/100g. The seeds were found to be rich in Ca, K, Na and P. The dominant fatty acids found in the oils were myristic and palmitic...
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Posted by admin on Dec 31, 2017 in |
Antimicrobial peptides (AMPs) are the crucial factors, which plays an important role in host defense mechanisms. AMPs are ubiquitous and found in diverse organisms ranging from microorganisms to animals. Plants are the precious source of natural antimicrobial molecules including antimicrobial peptides known as plant antimicrobial peptides (PAMPs). PAMPs can be divided into different families based on their, molecular weight, activity against different microbes, structure, charge of molecules, content of disulphide bond and mechanism of action. Based on number of cysteine residues and disulfide bonds, PAMPs are categorized into six main families. These peptides may lead to degradation of nutrients that are specific or essential for microbes to grow, interfering with microbial membrane or by conflicting with their metabolism. PAMPs exert multiple antimicrobial activities which includes membrane permeabillization, interference with DNA, RNA and protein synthesis that might provide a suitable approach to prevent bacteria from developing resistance. This review provides an overview of all the major plant AMP families including their structure, function, mechanism of action and antimicrobial...
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Posted by admin on Dec 31, 2017 in |
Cardiotoxicity associated with the clinically used drugs is a global concern of safety for healthcare professionals. Various animal models have been used to study the drug-induced cardiotoxicity but the exact molecular involvement of toxicity is not much clear. Despite the recurrent occurrence of toxicities, drugs such as doxorubicin, calcium channel blockers, antiarrhythmics and immunomodulators are regularly used. Anticancer drugs mainly anthracyclines, 5- fluorouracil and cyclophosphamide exert prominent cardio-toxicity. Till date, there is only one drug approved for doxorubicin-related cardiotoxicity i.e. dexrazoxane. Few other drugs are used routinely by clinicians to reduce the severity of toxicity which includes ACE inhibitors, L-carnitine, probucol, CoQ10, N-acetylcysteine, Vitamin E and deferoxamine, whereas antidepressants drugs, specifically tricyclic antidepressants are potential candidates for cardiotoxicity. Calcium channel blockers, antiarrhythmic and beta receptor antagonist aggravate cardiac heart failure (CHF) and left ventricular arrhythmia. Interferons, mainly interferon-α is also associated with prominent and dose-dependant toxicity. Some other drugs like zidovudine, chloroquine, cocaine, minoxidil, ketoconazole, prostaglandin E2 and anagrelide are also reported to have cardiotoxic effects. A complication associated...
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Posted by admin on Dec 31, 2017 in |
This study was conducted to evaluate the antimicrobial and cellular metabolic inhibitory properties of the ethanolic extract from the bark of ‘Lunas-bagon’, Lunasia sp. Extracts were evaluated using the agar well diffusion method for antimicrobial assay. The standard phytochemical screening was done for the determination of compounds that can be considered toxic and MTT assay for the effects of the extract on cellular metabolism. Results showed that the extracts have ability to inhibit selected Gram-positive and Gram-negative bacterial isolates indicating that the extracts are effective antibacterials. Results from the MTT assay revealed at doses lower than 100.0 ug/ml, extracts will not inhibit cellular metabolism of the splenocytes. Higher dose, however shows reduction of metabolic activity which may indicate the onset of apoptosis in cells. Basic biochemical tests indicated the absence of cyanogenic glycosides and anthraquinones, thus the extracts may not have toxic effects. Identification of bioactive compounds in through GC-MS analysis showed the presence of compounds that are known as antimicrobials and also effective in the treatment of selected...
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Posted by admin on Dec 31, 2017 in |
Rheumatoid Arthritis (RA) is a auto-immune disease in which body mistakenly considers some parts of its own system as pathogens and attacks them. RA is a chronic systemic inflammatory illness with prevalence of approximately 0.75% in India. It leads to irreversible joint damage and systemic complications. It is associated with substantial morbidity and increased mortality Better understanding of its pathophysiology has led to the progress of besieged therapies that have significantly enhanced outcomes. The key to beneficial achievement lies in identifying those who will have rigorous critical disease as early as possible, so that efficient management can be initiated prior to unalterable injure occurs. The primary aim of this study is to find out what factors play a significant role in determine the disease. From the brief account of discussion on observation and results of multivariate techniques are established, such techniques are important for they make it possible to encompass all the data from an investigation in one analysis. They in fact result in a clearer and better account...
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