Posted by admin on Dec 31, 2017 in |
Floating Drug delivery systems are designed to prolong the gastric residence time after oral administration. Ofloxacin is an antibacterial fluoroquinolone, widely prescribed in gastric and duodenal ulcers. It exhibits pH dependent solubility, more soluble in acidic pH and slightly soluble in neutral or alkaline pH. Precipitation of the drug occurs in the intestine, which adversely affects the absorption in the lower sections of the intestine. So there is a need for systems that reside in the stomach over a relatively long period and release the active compound in a sustained manner. The aim of the present study was to develop a delivery system wherein the retention of Ofloxacin could be achieved for increasing local action in the gastric region against Helicobacter pylori, with the development of rice bran oil entrapped zinc pectinate beads containing Ofloxacin. Various formulations of floating beads of Ofloxacin were developed using polymers like Low Methoxy Pectin alone and in combination with rate controlling polymers such as Gellan Gum, Karaya Gum and Xanthan Gum. The beads...
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Posted by admin on Dec 31, 2017 in |
Chitosan is a natural biopolymer that has been extensively used traditionally for the treatment of various ailments and as an efficient vehicle or carrier for fabrication, encapsulation and target-specific delivery of a wide range of pharmacological moieties for improved therapeutic efficacy. Despite of numerous characteristic features, the pharmaceutical significance of chitosan is limited due to its poor water solubility. The present study was aimed to investigate the impact of enzyme (hemicellulase)-mediated hydrolysis on the physicochemical characteristics including solubility, physicochemical nature, crystallinity, contact angle/wettability and surface morphology of the WSC. The sustainability of enzymatic method was evaluated by testing aqueous solubility and carrying out fourier-transform infrared spectroscopy (FTIR), x-ray diffraction (XRD), thermogravimetric analysis (TGA), contact angle measurement and scanning electron microscopic (SEM) analysis. Results of solubility studies revealed that hemicellulase-mediated hydrolysis significantly enhanced the aqueous solubility and wettability of the chitosan and exhibited smaller contact angle (θ) in comparison to the unmodified LMWCS. FTIR analysis showed that WSC synthesised via enzymatic hydrolysis exhibited ultra-low molecular weight and increased deacetylation degree...
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Posted by admin on Dec 31, 2017 in |
This study was carried out to isolate and characterize the bio active constituent from Euphorbia heterophylla and also to evaluate anticancer activity for the isolated compound against Breast cancer cell line (MCF -7 Cell line). The isolation of compound was performed on ethanol extract by using column chromatography and ultra sonicator by gradient elution. The structure of isolated compound was established on the basis of chemical test, thin layer chromatography and spectroscopic evidences. (IR, 1H NMR, 13C NMR, Liquid chromatography / tandem mass spectrometry (LC/MS/MS identification with data base library search). The yellow colour compound shows positive test for flavonoids in Thin layer Chromatography for flavonoids in the solvent system n-butanol: acetic acid: water (2:2:6) with Rf value 0.7. From the use of LCMS spectral library search system and TLC it is confirmed that the isolated compound is found to be quercetin. The Docking study was performed for the isolated quercetin against 5, 10 methenyltetrahydrofolate synthetase (MTHFS). The inhibition of (MTHFS) in human MCF 7 breast cancer cells has...
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Posted by admin on Dec 31, 2017 in |
Antibacterial activities, molecular docking and DNA cleavage activities of five structurally related Schiff bases [L3 (a), L3 (b), L3 (c) L3 (d) and L3 (e)] were performed and their structure activity relationship was studied. Antibacterial activities of these compounds against gram-negative and gram-positive bacteria were evaluated by using well diffusion method and the compound L3 (c); 1-[(E)-(4-hydroxyphenyl) iminomethyl] naphthalene-2-ol which carries two hydroxyl functional groups was observed as one of the most dynamic antibacterial agents. Docking interactions against p55blk kinase protein were investigated. The compound L3 (a); 1-[(E)-(3-nitrophenyl)imino-methyl]naphthalene-2-ol exhibited good docking proficiency with 24 interactions based on statistical potentials and the compound L3 (d); (E)-1-(2-methoxy-1-naphthyl)- N- (3-nitro phenyl) methanimine showed significant docking interaction based on hydrogen bonding. DNA cleavage efficiency of all the Schiff bases was investigated using Lambda DNA by gel electrophoresis...
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Posted by admin on Dec 31, 2017 in |
Hydrochlorothiazide and Candesartan cilextil are used in combination for the treatment of hypertension. The aim of the present investigation was to develop and validate a simple, efficient, economical RP-HPLC method for the simultaneous estimation of Hydrochlorothiazide and Candesartan cilextilin bulk and dosage forms. Chromatographic analysis was performed on Phenomenex C18RP column (250 x 4.6mm; 5µm) with mobile phase containing 10mM ammonium acetate: acetonitrile (65:35% v/v) at a flow rate of 1.2mL/min and eluents were monitored at 262nm. The retention times of Hydrochlorothiazide and Candesartan cilextil were 3.6 min and 6.8 min respectively and showed a good linearity in the concentration range of 7.8-18.8µg/mL for Hydrochlorothiazide and 10-26µg/mL for Candesartan cilextil with a Regression coefficient (R2) of 0.996 and 0.998 respectively. The validation parameters performed were specificity, linearity, limit of detection, limit of quantification, precision, robustness and stability. Validation parameters fulfilled regulatory requirements in all cases. The percentage recoveries ranged in between 98 – 102 (RSD < 2). The RP-HPLC method developed can be successfully used for the simultaneous estimation...
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