Posted by admin on Sep 30, 2016 in |
The purpose of this study examine whether the polymorphism in p53 upregulated modulator of apoptosis (PUMA) gene consequential treated with purified Nigella sativa lectin in tumor induced mice. Nigella sativa (N. sativa) is a widely used medicinal plant throughout the world. Possess a seed lectin that was purified by combination of ammonium sulfate precipitation and affinity chromatography on a Sepharose 4B column. The Nigella sativa strongly agglutinated human erythrocytes. The hemagglutinating activity of lectin was maintained after incubation at a wide range of temperature and pH and also was independent of divalent cations. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, lectin exhibited an electrophoretic profile consisting of a single band with apparent molecular mass of 45 kDa. Experimental groups were organized into 6 groups including 10 animals per each, additionally control group, blood samples were collected form mice before and after lectin treatment for liver and kidney enzymes biochemical analysis, Data statistical analysis for mitochondrial enzymes in our study showed a very highly significant (P>0.001) difference in the mean value...
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Posted by admin on Sep 30, 2016 in |
Thiamine hydrochloride, also known as Vitamin B1, is a water-soluble vitamin which is important biologically and pharmaceutically. A new simple and sensitive spectrophotometric method has been developed for the determination of Thiamine Hydrochloride (Vitamin B1) in pure form and biological fluids. The method is based on the diazotization reaction of Thiamine Hydrochloride with Procaine HCl which reacted with sodium nitrate and hydrochloric acid to form a red water-soluble azo dye in basic medium, that has a maximum absorption at λmax = 507 nm. Beer’s law is obeyed over the concentration range (2-60 µg.ml-1), the molar absorptivity of 4.047×104 L.mol-1 .cm-1 and Sandell sensitivity of 7.417×10-3 µg .cm-2 and the method have an accuracy and precision between (0.02-0.16) while the relative standard deviation between (0.805-2.049). The structure of dye showed that (1:2) product was formed between the drug and diazotized reagent. The method was applied successfully for the determination of Vitamin B1 in pharmaceutical preparations and biological samples. The results showed that the concentration of vitamin B1were decreased, in both...
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Posted by admin on Oct 1, 2015 in |
Objective: The aim of this study is to evaluate anti-oxidant and anticancer activity of methanol extract and ethyl acetate soluble fraction of methanol extract of Symplocos racemosa bark against the hepatocellular carcinoma. Materials and methods: DPPH and H2O2 free radical scavenging assay were tested for determining the anti-oxidant activity. Rat normal liver cells (BRL-3A) and human hepatocellular carcinoma (Hep3B) cells were tested in-vitro for cytotoxicity using (3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. Result: Ethyl acetate soluble fraction of methanol extract of Symplocos racemosa bark exhibited significant DPPH and H2O2free radical scavenging activity withIC50 value (µg/ml) of 10.58 and 11.89 as compared to standard drug ascorbic acid. Ethyl acetate soluble fraction of methanol extract of Symplocos racemosa bark exhibited significant cytotoxicity against human hepatocellular carcinoma (Hep3B) cells in-vitrowithIC50 value (µg/ml) of 32.55 as compared to standard doxorubicin (55.43µg/ml), and not affected the normal liver (BRL-3A) cells. Conclusion: Symplocos racemosa bark showed the potent anti-oxidant and anticancer activity. It may be due to presence of phytochemicals which are responsible for the anticancer...
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Posted by admin on Oct 1, 2015 in |
In this study, we assessed the availability, cost and affordability of essential antibiotics for paediatrics in a semi-rural region in Tanzania. Data were collected from 1 Medical Stores Department (MSD), 8 public hospitals, 30 Private Pharmacies and 30 Accredited Drug Dispensing Outlets (ADDO`s). The median price of tracer medicines and average stock-out days of per year were used to assess the cost and availability of medicines in the health facilities, respectively. The salary of the lowest paid unskilled government worker and national poverty line income were used to assess affordability of medicines. The median availability of the lowest-price generics of essential antibiotics for paediatrics in the pharmacies, ADDO’s and public hospitals was 59.09%, 62.5%, and 45.5%, respectively. At MSD, only 13 out of the 22 antibiotics were in stock at the time of survey. The median stock out days was 124 days per year. The median price ratios (MPRs) of retail patient’s prices for 20 generic antibiotics were 1.774 for 30 pharmacies, while for 8 generic antibiotics in 30...
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Posted by admin on Oct 1, 2015 in |
Objective: to investigate the anti diabetic effect of ethanolic extract of Cinnamomum zeylanicum on Alloxan induced diabetes in experimental animals (Rats). Materials and Methods: the alcohol extract of Cinnamomum zeylanicum was tested for its efficacy in Alloxan (150mg/kg) induced diabetic rats. The diabetic rats were divided into 5groups. Group I (control) received 2% gumacasia, group II (positive control) received standard drug Glibenclamide (10mg/kg+2%GA), group III, IV, V (T1 T2 T3) were treated orally with a daily dose of 50mg/kg, 100mg, 200mg, respectively for 30 days, for all diabetic rats after giving TEST, NC, PC preparations, the blood samples were collected & the blood glucose levels determined at 0, 1…, 0 hr reading is before giving the drug & the remaining three readings after giving the drugs. 24th hr reading is considered as 0hr reading for the next day. Results: administration of alcohol extract of Cinnamomum zeylanicum produced a dose dependent decrease in blood glucose levels in Alloxan induced rats. There was significant fall in blood sugar level in High...
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