Posted by admin on Sep 30, 2020 in |
Active Pharmaceutical ingredient (API) of pharmaceutical bulk drug Brompheniramine Maleate (API) was validation by gas chromatography. Aim for this article was to check the Residual Solvents in Brompheniramine Maleate (API) by Gas Chromatographic technique. To validate a Gas Chromatographic method for detection and Quantification of Residual Solvents Methanol, Isopropyl Alcohol, DMSO and -O-Xylene. This technique was developed accurately, and validation parameters such as Accuracy, Specificity, Precision, Linearity and Range, Limit of detection (LOD), Limit of quantitation (LOQ), ruggedness, robustness and system suitability testing are explained. Gas chromatograph equipped with FID detector and headspace injection and column 30 m × 0.32 mm ID × 1.8 µm DB-624 capillary column with column temperature was 40 °C (hold 10 min) to 250 °C @ 40 °C/min, hold at 250 °C for 5 mins. Specificity was retention time for Methanol (2.13), Isopropyl Alcohol (3.45), DMSO (13.90), and -O-Xylene (13.74). The recovery for Methanol % RSD was 2.49, Isopropyl alcohol 1.18, and O-Xylene 1.91. All validation parameters are used in the routine and stability...
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Posted by admin on Sep 30, 2020 in |
The secondary plant metabolites possess therapeutic value, thus play a significant role in human health and general wellbeing. The present study aimed to estimate the secondary metabolite concentration and to evaluate the antioxidant potential of Tithonia diversifolia leaf and flower extract in methanol. The phytochemical compounds in the extracts were characterized through Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The leaf extract exhibited sixteen bioactive compounds, and flower extract revealed thirteen compounds with valuable activity. The major phytocompounds in leaf extract were pentacosane (22.01%), gamma- sitosterol (15.80%) and pentatriacontane (11.95%) whereas in flower extract showed methyl linolelaidate (19.55%), methyl palmitate (18.73%), 1- dotriacontanol (15.35%), 5- eicosene, (E)- (11.90%). The compounds were found to be dissimilar in both leaf and flower. Quantitative phytochemical analysis of leaf extract was found to contain a high concentration of phenols, flavonoids and saponins, whereas total alkaloids were maximum in flower extract. In in-vitro, the antioxidant activity was evaluated using 1, 1-diphenyl-2- picrylhydrazyl (DPPH) assay. Both leaf and flower extract showed remarkable antioxidant scavenging activity with an...
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Posted by admin on Sep 30, 2020 in |
Objective: The aim of this study was to screen various solvent extracts of leaves of Rumex hastatus to display in-vitro antioxidant activity, total phenolic, and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. Materials and Methods: A detailed study was performed on antioxidant activity of various Rumex hastatus leaves extracts like petroleum extract (PERH), chloroform extract (CERH), ethyl acetate extract (EAERH), methanolic extract (MERH) and aqueous extract (AERH) by in-vitro chemical analysis like DPPH, Reducing power, metal chelating, hydrogen peroxide and nitric oxide free radical scavenging assay. All these extracts were also subjected to a preliminary phytochemical screening test for various constituents. The total yield, total phenolic, and total flavonoid contents of all extracts were also determined. Results: Phytochemical screening shows the presence of triterpene, flavonoids, polysaccharides, steroids, tannins, cardiac glycoside, reducing sugar, and saponins in all extracts. The MERH exhibited the highest total phenolic content (121.7 ± 5.95 mg of GAE/g extract) and total flavonoid content (165.9 ± 3.99...
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Posted by admin on Sep 30, 2020 in |
Diabetes mellitus is the heterogeneous metabolic disorder caused by the high blood sugar level. Untreated high blood sugar can damage kidneys, eyes, nerves and other organs. Type 1 diabetes mellitus disorder is caused by the lack of insulin hormone while type 2 diabetes mellitus is a disorder of insulin resistance by β cells of the pancreas. For the management of type 2 diabetes mellitus different drugs are available as single or combination forms like Pioglitazone, Repaglinide, Metformin, Voglibose, Glipizide. Several research activities were carried out for its development, formulation and evaluation by the development of controlled-release and fast-dissolving formulations. The extensive literature review revealed information related to the formulation of sustain/fast release dosage form for the antidiabetic drugs. Newer techniques were used by the researcher for the formulation of dosage forms as solvent diffusion-evaporation technique, Reverse phase evaporation technique, emulsion solvent evaporation technique and Hot melt extrusion granulation technique. The review represents the types of formulation, methods used, excipients used and evaluation parameters of developed dosage forms and their...
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Posted by admin on Sep 30, 2020 in |
A Simple and trace level-sensitive Gas chromatography with mass spectrometer (GCMS) method was developed, optimized and validated for the determination of seven potentially genotoxic impurities which are having structural alerts i.e., 1-Bromo-5-chloropentane (PGI-1), Ethyl-6-chloro-2-oxo hexanoate (PGI-2), Methyl-7-chloro-2-oxo hepta-noate (PGI-3), Ethyl-7-chloro-2-oxo heptanoate (PGI-4), Ethyl-7-bromo-2-oxo heptanoate (PGI-5), Ethyl-8-chloro-2-oxo octanoate (PGI-6), Ethyl-2-acetyl-7-chloro heptanoate (PGI-7) contents in Cilastatin sodium drug substance. The chromatographic separations of seven genotoxic impurities were achieved on ZB-5 MS [5% Polysilarylene, 95% Poly-dimethylsiloxane copolymer capillary column of 30 m length, 0.32 mm internal diameter, film thickness 1.0 µm]. The analytic method has been demonstrated through method validation experiments w.r.t ICH guidelines, like specificity, linearity, Limit of Detection, Limit of Quantification, method precision and accuracy (recovery) parameters with allowable TTC limit. The achieved limit of detection (LOD) values was 3 µg/g. This developed method was found to be linear with correlation coefficient is greater than 0.999. Developmental and validation experiments were discussed in detail in this...
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