Posted by admin on Jul 31, 2016 in |
A reverse-phase liquid chromatographic (RP-HPLC) method was developed for the determinations of loperamide hydrochloride and norfloxacin in their marketed formulation and bulk. The separation was carried out using mobile phase of triethylamine and acetonitrile (50:50%) with pH 4.The pH adjusted with orthophosphoric acid. The column used was Capcell pack C18 Column (250mn x 4.6mm, 5mm) and flow rate of 1 ml/min. Detection carried out at 213 nm. The retention time loperamide hydrochloride 5.6 and norfloxacin were found to be 2.1 min respectively. Developed method was validated according to ICH guideline. Linearity was observed at concentration rang of 2-6 mg/ml for loperamide hydrochloride and 200-600 mg/ml for norfloxacin. The regression equation were found to be Y=48615x-435565 and Y=72087x-14016 the correlation coefficient (r2) 0.9996 and 0.9988 norfloxacin and loperamide hydrochloride respectively. The percentage RSD for the method precision was found to be less than 2%. The accuracy is found in 98-101 %. The proposed method is precise, accurate, selective and rapid for simultaneous determination of loperamide hydrochloride and...
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Posted by admin on Jul 31, 2016 in |
Azima tetracantha is a small flowering shrub belongs to salvadoraceae family and commonly known as mulluchangu or mulsangu in Tamil. It is an best known medicinal plant ,which have a central role in the treatment of disease from pre historical period. From the plant lot of phytochemicals has been reported such as alkaloids, steroids ,tannins, terpenoids, flavonoids, glycosides, phenols etc and also possesses activities such as antioxidant, antifungal, antibacterial , hepatoprotective, antidiabetic and antihyperlipidemic etc .In the present study preliminary evaluation and isolation of an active pharmacological compound euphol was obtained from the chloroform extract of the leaves of Azima tetracantha was carried out. The compound euphol is a tetracyclic triterpene alcohol which produces antinociceptive effects in inflammatory and neuropathic pain. The compound was isolated by column chromatography and characterized by chemical test, TLC, FTIR, NMR, C 13 NMR spectrum. In future it may be a promising molecule for the management of neuropathic...
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Posted by admin on Jul 31, 2016 in |
The Wheat, Maize and Cotton are the major crops in the agriculture field specially in Maharashtra, India. So for better productivity this study aims to prepare steam soluble aroma compounds called hydrosols. Hydrosols of different plant species (Ipomea carnea Leaves and Flowers, Lantana Camera Leaves) were prepared by steam distillation method. The purpose of this study was to determine the effect on the growth of Wheat, Maize and Cotton crops by using Ipomea carnea leaves and flowers and Lantena camera leaves. In this study the wheat, maize and cotton crops were germinated in different pots and continuous dosing of hydrosol was applied with fixed quantity and result was observed after...
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Posted by admin on Aug 1, 2015 in |
Present investigation deals with the phytochemical and antioxidant studies of Justicia gendarussa Burm.f. (family Acanthaceae), a medicinally important herb. Biochemical studies highlighted that in leaf tissue, quantities of soluble carbohydrate, amino acid and ascorbic acid are of 307 mg/g, 34.93 mg/g and 619µg/g, respectively. In stem bark, quantities of soluble carbohydrate, amino acid and ascorbic acid are of 185 mg/g, 26.86 mg/g and 330.33µg/g, respectively. Pigment content in dried leaf has been estimated as chlorophyll a 46µg/g, chlorophyll b 16.1µg/g and total carotenoids 16.7µg/g. In bark, amounts of chlorophyll a, chlorophyll b and total carotenoids are of 23.9µg/g, 21 µg/g and 14.6µg /g, respectively. Enzyme content in fresh leaf showed that amount of the catalase is 4.89 EU/ g fresh tissue and in bark it is 2.39 EU/ g. Peroxide is 3.4 EU/g for fresh leaf and 2.21 EU/g in stem bark DPPH radical scavenging activity was considerably high for methanolic extract of leaf that is 31.36 % and in case of bark the activity was 23.74 %. The...
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Posted by admin on Jul 31, 2015 in |
Methylmercury (MeHg), a highly toxic environmental pollutant, induces oxidative stress and dysfunction in many cell types. Bacopa monniera is a perennial herb, and is used as a nerve tonic in ayurveda, a traditional medicinal system in India. This study was aimed to evaluate the effect of Bacopa monniera extract (BME) on MeHg-induced toxicity in rat cerebellum. Male Wistar rats were administered with MeHg orally at a dose of 5 mg/kg b.w. for 21 days. Experimentalrats were given MeHg and also administered with BME(40 mg/kg, orally) 1 h prior to the administration of MeHg for 21 days. After treatment MeHg induction caused increased level of Scgb3a1mRNA whereas the level was reduced by BME pretreatment. GPx4 expression was down regulated and HSP70 expression was up regulated in MeHg intoxicated group. Immunohistochemical changes were observed in TRxR1 and GFAP protein in MeHg induced group. BME pretreatment altered the expression of proteins and Immunohistochemical changes to near normal. These findings strongly implicate that BM has potential to protect brain from oxidative damage resulting...
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