Posted by admin on Jul 31, 2018 in |
Marine environment is a huge reservoir of various important resources for new bioactive molecule discovery from marine organisms. The aim of this investigation is to evaluate the anti-proliferative potential of crude proteins extracted from different organs of Donax variabilis (gills, adductor muscles, mantle and whole tissue) on human Lung (A549), oral (KB3-1), breast (MCF-7) and colon (HT-29) cancer cell lines. The crude proteins extracted from four different organs of marine clam were biochemically and qualitatively analysed using Native and SDS PAGE. These extracts were tested against normal (vero) cell line to determine the toxicity of crude proteins. Among the four organs, proteins from mantle potentially inhibited growth of lung, oral and breast cancer cell lines in a dose dependent manner and inhibition of growth in colon cancer cell line was time dependent when examined by MTT assay. Significant inhibition of cell viability on A549 cells observed at (IC50) 100 µg/ml, KB 3-1, MCF-7 cells at (IC50) 250 µg/ml concentration after 24 h of incubation and HT-29 at (IC50) 500...
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Posted by admin on Jul 31, 2018 in |
The main objective of the study is to evaluate orodispersible tablets of primaquine with different in-vivo parameters. Sharma P et al., (2015), in-vitro study was based on various evaluation parameters of orodispersible tablets of primaquine. From their study results, we have selected formula-6 of orodispersible tablets of primaquine for in-vivo study. Taste masking evaluation was performed on five male Swiss Albino rats (200-250 g). The in-vivo bioavailability study of drug were performed on six male white New Zealand rabbits (weighing 2-2.5 kg) and rabbits were randomly divided into two groups of equal size using parallel design. Next step of the study was HPLC analysis, in this study two conditions were followed by us. The conditions were- (a) Sample preparation: Protein precipitation method selected for extraction of drug from plasma because it was very simple and cost effective method for primaquine extraction. (b) Chromatographic conditions: HPLC-DAD-UV (Shimadzu Class-VP Japan) was used for evaluation of chromatographic conditions. With the help of UV and HPLC, marketed preparation (Primacip film coated tablet) and...
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Posted by admin on Jul 31, 2018 in |
Cordia dichotoma Linn. belonging the family Boraginaceae is locally called as Lasora in India. It is an important drug to cure diseases of the kidney, liver, spleen, heart and blood which attribute many medicinal properties in Ayurveda. The purpose of this study is to identify and characterize the bioactive principle from the bark of Cordia dichotoma Linn. Phytochemical screening methanolic extract of bark of Cordia dichotoma Linn. was done and showed the presence of phytosterol. Phytosterols were isolated from the methanol extract by admixing 100 g of extract with 200 ml of acetonitrile. The resulting white sterol crystals were analyzed by Thin Layer Chromatography and High Performance Liquid Chromatography (HPLC) for the purification. The sterol for structure elucidation was further studied by more sophisticated analytical techniques e.g. FTIR, 1H-NMR, 13C-NMR and LC-MS. After various spectral analysis and interpretation of same, the isolated compound was identified as...
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Posted by admin on Jul 31, 2018 in |
Dexamethasone drug induced the hyperglycemia in the Albino mice when intra-peritoneally administered at the dose of 10 mg/kg. Administration of dexamethasone raised the level of blood sugar in the mice. Administration of aqueous extract of C. citratus root and flower extract reduced the fasting and postprandial blood sugar levels, bringing them towards normal. We were observed anti-diabetic effect both root and flower extracts but the root had better anti-diabetic...
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Posted by admin on Jul 31, 2018 in |
An object with at least one characteristic dimension between 1 and 100 nm can be defined as “nanomaterial”. The unbelievable development of nanotechnology in the last 30 years has allowed the release of new and efficient synthetic routes towards the production and functionalization of different NPs, composed of a variety of materials including noble metals (e.g. gold and silver), semiconductors, TiO2, magnetic compounds and their combinations, such as core-shell and alloy. Advances in nanoparticle synthesis and engineering have produced nanoscale agents affording both therapeutic and diagnostic functions that are often referred to by the portmanteau ‘Nanotheronostics’. The field is associated with many applications in the clinic, especially in cancer management. These include patient stratification, drug-release monitoring, imaging-guided focal therapy and post-treatment response monitoring NPs which is clearly elaborated in this work. Intelligent nanotheronostics based on bio responsive systems have recently emerged and offer the promise of high specificity and efficiency via “on-demand” activation of both therapeutic and diagnostic capabilities are being widely used for cancer diagnostics. As this development...
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