Posted by admin on Sep 1, 2012 in |
To analyse the situation of antibiotic resistance, a total of 77 E. coli isolates from urine, pus, sputum and endo-tracheal aspirate were screened for their antibiograms for antibiotic resistance, multiple antibiotic resistance (MAR) index for evaluating the spread of resistance and plasmid profiles for the presence and characterization of plasmids. Very high resistance level (>90%) was detected against ampicillin, amoxicillin, ceftazidime, norfloxacin, tetracycline while imipenem and amikacin recorded the least resistance levels of 2.3% and 13.9% respectively among the isolates. An increased resistance to amoxicillin, tetracycline, cotrimoxazole and norfloxacin were observed in this geographical area which however displayed a lower resistance in other countries. The MAR index varied considerably, the lowest was 0.18 and the highest was 0.89. Plasmids of 10 size ranges were detected in the isolates. Some isolates possessed single sized plasmid while other possessed multiple plasmids. Isolates with high multi- antibiotic resistance profiles were found to possess multiple plasmids. This study shows that regular antimicrobial sensitivity surveillance is necessary and acquisition of plasmid could greatly contribute...
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Posted by admin on Sep 1, 2012 in |
The methanol extract of Centella asiatica (Family:Umbelliferae) was studied for its phytochemical and pharmacological Activities. This study of the extracts indicates the presence ofReducing Sugar Tannins, Alkaloids, Sapponins and.The pharmacological study include Antimicrobial activities.The extract of the plant showed activity against a wide a variety of microorganism’s .All the activities were compared by measuring the zone of inhibition with the standard antibiotic. Based on the findings of Antimicrobial activity, we can say that the plant may contain novel compounds that possess potent anti-mutagenic activity and anti-oxidative activity. The obtained results support for the uses of this plant as traditional medicine. Infectious diseases are the second leading cause of death worldwide. Treatment of infections continues to be problematic in modern time because of the severe side effects of some drugs and the growing resistance to antimicrobial agents. The activities observed could be attributed to the presence of some of the phytochemicals detected which have been associated with antibacterial...
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Posted by admin on Sep 1, 2012 in |
A Reversed Phase Ultra Performance Liquid Chromatographic (RP-UPLC) method was developed for the determination of glipizide (GLI) in in-vitro study during formulation development. The chromatographic separation was achieved on a Acquity UPLC BEH C18 (50 mm × 2.1 mm, 1.7 µm) column, with a mixture of 55 volumes of methanol and 45 volumes of buffer contain 0.001M sodium dihydrogen phosphate in isocratic elution with flow rate of 0.3 mL/min. The eluted compound was monitored at a wavelength of 276 nm using a UV detector. The method described herein separated glipizide and glipizide related compound-A from all other formulation components within a run time of 1.2 min. The RP-HPLC method was developed and validated according to the International Conference on Harmonization (ICH) guidelines. This method was successfully applied in the quantitative determination of glipizide in in-vitro study during formulation development. The procedure described herein is simple, selective, and reliable for routine quality control analysis as well as stability...
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Posted by admin on Sep 1, 2012 in |
A direct spectrophotometric method was developed by the authors for the detection and determination of satranidazole in pure form as well in pharmaceutical formulations in the form of tablets and capsules. The method was based on the formation of a reddish-purple color dye, due to the diazotization reaction between the nitro group of the drug sample, sulphanilamide and NEDA. The drug sample dissolved in hot water, followed by the addition of 2ml each of 0.5% Sulphanilamide and 0.3% NEDA. It exhibited a stable instantaneous reddish purple, colour, which showed maximum absorbance at 540nm. Beer’s law was found to be obeyed in the range 50-300 µgmL-1, with a limit of detection of 0.09 µgmL-1. The method was found to be simple, accurate and...
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Posted by admin on Sep 1, 2012 in |
Phosphatase serves several functions in plant metabolism including growth governance, phosphorous level control, starch breakdown etc. Alkaline phosphatases, acting at an alkaline pH 8, are a significant class of enzymes that catalyze release of phosphate esters especially. This enzyme study is so far limited only to animal source and partly to microbial sources, in terms of clinical research. Although it has been identified that plant as a source of this enzyme may be exploited, there always has been a challenge on the isolation and characterization of this enzyme and how pure it can be. This paper partly addresses the above problem, where the enzyme has been isolated from the seeds Dolichos lab-lab plant characterized and its purity was checked by HPLC. The purity obtained was 98% and the enzyme has been further analyzed for its activity on nucleic acids, which gave promising and positive...
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