Posted by admin on Sep 1, 2012 in |
Crinum is a genus of about 130 species belonging to family Amaryllidaceae with wide geographical distribution throughout the tropics, subtropics and warm temperate regions of the world. These plants are not only showy ornamentals but they possess significant folkloric and commercial reputation as well. Long ago, Crinums have been subjected to extensive chemical, cytological and pharmacological investigations. Phytochemical investigations have resulted in isolation of several diverse classes of phytocompounds and have been focused predominantly on alkaloids. The present part of our review work about the phytochemical, biological and toxicological studies on Crinums summarizes crinine-type alkaloids isolated up to now as well as their structural and stereochemical differences, in addition to their distribution in different Crinum...
Read More
Posted by admin on Sep 1, 2012 in |
L-Asparaginase (E.C. 3.5.1.1.), also known as L-asparagine amidohydrolase is the enzyme with anti-tumor activity and is well accepted as a chemotherapeutic agent against the acute lymphoblastic leukemia and lymphosarcoma. This article has briefly touched nearly all the industrial and clinical aspects of L-Asparaginase and provides the recent update of the topic. The article includes a brief introduction to the topics, mechanism of action, a little information about the structure, sources of enzyme, purification, optimum conditions for the enzyme production, recombinant strains for higher productivity and formulation of the...
Read More
Posted by admin on Sep 1, 2012 in |
Helminthiasis is infestation with one or more intestinal parasitic worms roundworms, whipworms , or hookworms in humans and animals. Presently, many synthetic drugs are available to treat Helminthiasis infection effectively, but suffer from number of side effects like abdominal pain, dizziness, headache, fever, nausea, vomiting, or temporary hair loss. Moreover, drug resistance is also another factor concerned with the use of these drugs. Thus, Herbal drugs need to be introduced as large number of medicinal plants are known for their antihelmintic activity with fewer or no side effects and are used by the ethnic groups across different parts of the world. But these medicinal plants need to be screened first for their in vitro and in vivo activity before putting them in use. Thus, many of these herbs have been scrutinized for their pharmacological and pre clinical studies. The present article describes review of promising in vitro efficacy of some medicinal plants having antihelmintic activity, which can be helpful in investigation and discovery of novel herbal...
Read More
Posted by admin on Sep 1, 2012 in |
Allium sativum L. (Liliacea) is a perennial bulb with a tall, erect flowering stem. The bulb of the plant has been used in many parts of the world as a stimulant, carminative, antiseptic, expectorant, anthelmintic and diuretic. This study has been planned to assess the diuretic activity of fresh garlic bulb extract targeting the steroidal and triterpenoidal saponin content. The rats were randomly divided into 4 groups of 5 animal each as vehicle control (2 % tragacanth suspension), standard drug frusemide (20 mg/kg, p.o), and n-butanol extract (10 mg/kg and 20 mg/kg, p.o) treated. Urine was collected in a graduated cylinder and its volume was measured for next 5 hr. Na+, K+ and Cl– concentrations were measured. Phytochemical analysis of A. sativum n-butanol fraction showed presence of steroids, triterpenoidal saponins and carbohydrates. At 20 mg/kg dose onset of diuresis and total volume of urine formed was significantly (P<0.01-0.05) higher. Fifth hour urine volume at 20 mg/kg dose was 9.3 ml as compared to 5.5 ml of control. Extract at...
Read More
Posted by admin on Sep 1, 2012 in |
A simple, sensitive and reproducible method was developed and validated for the simultaneous estimation of Diethylcarbamazine and Levocetirizine in its tablet formulation by reverse phase high performance liquid chromatography using Waters1515 HPLC with Diethylcarbamazine and Levocetirizine simultaneously. HPLC-PDA detector at the λmax of 253nm, using Hypersil-BDS C18 (250×4.6 mm. 5 μ) column. The mobile phase used was potassium dihydrogen orthophosphate buffer (pH: 5): acetonitrile (20:80 v/v) with isocratic flow (flow rate 1 ml/min) and the pH was adjusted with orthophosphoric acid. The compounds Diethylcarbamazine, Levocetirizine were eluted at 2.04, 5.54 min, respectively. The peaks were eluted with better resolution. The method was accurate with assay values of 99.67% and 99.81% w/w, precise (%RSD) with 0.3and 0.5, percentage recovery values of pure drug were in between 99.4% to 100% and 99% to 99.4% which are very sensitive with limit of detections (LOD)’s 2.42 and 0.08ppm and limit of quantification (LOQ)’s 7.42 and 0.2ppm, these results are within the range of limits 98% to 101% which indicates that the method was...
Read More
