Posted by admin on Aug 31, 2018 in |
From time immemorial pharmacologists and researchers have always been in search for novel drug screening methods. One such unique technique that is gripping the world by its leash is “human tissue chip technology”. This study explores the creation of Multiple Sclerosis (MS) using human tissue chip and assessing the ability of Vasicine a quinazoline alkaloid derived from Adhatoda Justiciae plant in reversing the inflammatory pathology of multiple sclerosis. 10 ml of human peripheral blood was withdrawn using a sterile syringe from a healthy donor. The blood was centrifuged to obtain mesenchymal stem cells (MSC). Then the MSCs were transferred with Dulbecco’s Modified Eagle’s medium (DMEM). After 48 h incubation, the MSCs were transferred into four flasks with growth factors and were seeded into tissue chip for differentiation into neuronal cells, cardiomyocytes, hepatocytes, and skeletal muscle cells. The respective cells were dislodged from tissue chip for histopathological evaluation and differentiation confirmation. Lipopolysaccharide (LPS) was added to these cells, to mimic MS inflammation and was confined to histo-pathological assessment. 9 biomarkers...
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Posted by admin on Aug 31, 2018 in |
Effervescent tablet is a convenient oral dosage form, which has special ingredients. This potency of effervescent tablet helps to delivered drugs, effectively. Medicinal plants have a wide range of usage in the world. Mespilus germanica (Rosaceae) and Quercus castaneifolia (Fagaceae) are important medicinal plants used in several diseases such as diarrhea. Medlar and oak, the fruits of M. germanica and Q. castaneifolia respectively, were harvested and extracted using hydro-acetonic solvent by maceration method. In addition, total phenol and tannin contents were assayed using Folin-Ciocalteu method. Antimicrobial activity of extract was determined using broth dilution test on some intestinal pathogens. Finally, effervescent tablets were prepared using wet granulation method and analyzed. Total phenols and tannins were obtained 0.9% and 0.1% respectively. This reduces the amounts of total tannins will disappear with adding antioxidant agents. Tablet hardness, friability and disintegration time were significantly dependent on the amounts of lubricant, acidifying and alkalizing agents. This work investigated the potential of developing Oak and Medlar effervescent tablets to facilitate drug administration and mask...
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Posted by admin on Aug 31, 2018 in |
We have developed Ag2Ga nanoneedles and achieved individual nanoneedles with diameter ranging from 200 to 250 nm with sharp tip and micro scale length required for a drug carrier to deliver drugs to site of application. Nanoneedles are designed to be inserted in human tissue and therefore they have to be compatible with the local environment of both in terms of toxicity and intended function. The variation of toxicity results for various nanostructures were reported in literatures. Hence, it is important to assess the information about toxicity of novel needle shaped nanostructures. Acute toxicity study was performed to examine the acute dermal toxicity of Ag2Ga nanoneedle mediated silver gel formulation on the rat’s skin, according to the OECD Guidelines Test no.402. Additionally, acute dermal irritation study was also performed to examine the irritancy potential following single, 1 h and 4 h, semi occluded application of Ag2Ga nanoneedle preparation on the rat’s skin according to OECD Guidelines Test no. 404. Acute toxicity study observations showed no skin toxicity or mortality...
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Posted by admin on Aug 31, 2018 in |
Lymphatic Filariasis is one of the most abandoned tropical diseases caused by the parasite, Brugia malayi. The existing conventional drugs act generally on the larval stages of the parasite. The enzyme asparaginyl tRNA synthetase is an excellent molecular target as it plays a crucial role in protein synthesis. Evidences based on the literature presented clues to discover the flavonoids as potential anti-filarial leads, which led to the scope for this computational study. The computational parameters such as docking score, binding energy, intermolecular hydrogen bond interaction and the identical amino acids confirm that flavonoids could serve as prospective anti-filarial agents. The outcomes prove that they can be further explored in in-vitro and in-vivo studies to authenticate their claim as potential anti-filarial...
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Posted by admin on Aug 31, 2018 in |
About 70 middle aged (20 – 45 years) patients, including 40 males and 30 females, operated at Base Military Hospital, Meerut Cantonment Board, Meerut (India) developed typical symptoms of pus formation at surgical sites and opening of sutures after 30 – 45 days. To investigate the causative pathogen and source of infection, various samples were collected from wounds and various sites of Operation Theatre. The samples were processed on culture media including nutrient agar, McConkey agar and Lowenstein-Jensen’s medium (LJ medium). To identify the pathogens, Gram’s and acid fast staining of the smears of samples and cultures obtained on different media, were carried out. Acid fast bacilli were observed after 3 – 5 days of incubation on LJ medium. The wound samples showed the prevalence of Mycobacterium chelonae and acid fast...
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