Posted by admin on Oct 31, 2025 in |
Plants have evolved to produce a vast array of natural compounds with significant health-promoting properties. Medicinal plants remain crucial resources for contemporary drug discovery and development. This study presents the first scientific investigation into the medicinal potential of Syzygium tamilnadense (Rathakr & Chitra), a rare and endemic species of the Myrtaceae family native to the Sholur–Nanjanad Reserve Forests in the Nilgiri Biosphere, Western Ghats, Tamil Nadu, India. Various solvent extracts were prepared, with ethanol yielding the highest concentration of phytochemicals. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, catechins, coumarins, glycosides, phenols, saponins, steroids, tannins, terpenoids, sugars, and xanthoproteins compounds known for their diverse pharmacological activities. Quantitative analysis showed high contents of total phenolics (128.14±0.76mg GAE/g), flavonoids (44.20±0.28mg QE/g), and tannins (38.26 ± 0.48 mg QAE/g), indicating strong antioxidant capacity. Gas Chromatography– Mass Spectrometry (GC–MS) analysis identified 25 bioactive compounds, including caryophyllene oxide, neophytadiene, squalene, phytol, and linoleic acid ethyl ester, which are associated with antioxidant, anti-inflammatory, antimicrobial, and anticancer properties. These findings highlight S. tamilnadense as novel...
Read More
Posted by admin on Oct 31, 2025 in |
Sulphacetamide is a sulfonamide antimicrobial widely used in injectable formulations for ocular and systemic bacterial infections. However, its chemical sensitivity to environmental factors poses a significant challenge for formulation stability, requiring a comprehensive impurity profiling approach. The current study aimed to develop and validate a stability-indicating UPLC-ESI-MS/MS method for the identification and characterization of impurities and degradation products of Sulphacetamide under various stress conditions in accordance with ICH Q1A (R2) guidelines. Forced degradation studies were performed by subjecting Sulphacetamide to acidic, basic, oxidative, thermal, and photolytic stress. Chromatographic separation was achieved using a BEH C18 column with an optimized mobile phase under gradient elution. Mass spectral data were acquired using electrosprayionization in positive mode, enabling structural elucidation of degradation products based on fragmentation patterns and m/z values. The method showed excellent specificity, sensitivity, and resolution for Sulphacetamide and its impurities. Major degradation was observed under acidic and oxidative conditions, yielding characteristic fragments such as m/z 131.38, 149.54, 200.02, 325.42, and 425.72. Minor degradation was noted under basic conditions, while...
Read More
Posted by admin on Oct 31, 2025 in |
Background: Poor management of biomedical waste (BMW) poses severe health and environmental risks, especially during the COVID-19 pandemic that saw an excess of infectious waste. The Biomedical Waste Management Rules of 2016, subsequently amended in 2018, provide guidelines for safe disposal; however, there remain considerable gaps in knowledge among healthcare personnel. The present research assessed and improved their knowledge, attitude, and practice (KAP) about COVID-19-related BMW via a structured teaching program (STP) at a tertiary care hospital in Northern India. Methodology: The quasi-experimental one-group pre-test post-test design was utilized in 50 health workers. Pre- and post-intervention KAP scores were quantitatively measured, correlations be-tween demographic parameters such as age, education, and clinical experience were compared. Findings: A majority of the participants were aged 25–34, 56% of them had a master’s degree, and 14% had 6–10 years of experience. Pre-intervention scores were as follows: knowledge – 70.83%, attitude – 80.83%, practice – 67.08%. Post-intervention, the knowledge increased to 78.83%, the attitude improved to 84.16%, and practice decreased slightly to 65%. There...
Read More
Posted by admin on Oct 31, 2025 in |
This study investigates the imaging characteristics of testicular tumors through the use of Doppler sonography, emphasizing its significance as a first-line, non-invasive diagnostic modality for the evaluation of testicular masses. Testicular tumors represent a significant clinical concern due to their potential malignancy and impact on male reproductive health. High-resolution ultrasonography combined with color and spectral Doppler imaging, offers comprehensive insights into both the morphological structure and vascularity of testicular lesions. This dual approach enhances the clinician’s ability to distinguish between benign and malignant pathologies. The study presents a detailed analysis of seven clinically suspected cases of testicular tumors, encompassing a range of histopathological types such as lymphoma, sex cord-stromal tumors, and various non-seminomatous germ cell tumors (including yolk sac tumors, embryonal carcinomas, teratomas, and mixed germ cell tumors), as well as seminomas. Sonographic features observed in malignant tumors include heterogeneous echotexture, increased internal vascularity, and the presence of retroperitoneal lymphadenopathy, which are key indicators of aggressive disease. The findings demonstrate that Doppler ultrasound is not only effective in the...
Read More
Posted by admin on Oct 31, 2025 in |
In this study, a series of novel N-(2,4′-dioxospiro[indoline-3,2′-thiazolidin]-3′-yl) isonicotinamide derivatives (VIa-l) were synthesized by cyclizing N1- (2-mercaptoacetyl) isonicotinohydrazide with various 5,6,7-substituted isatins. Structural confirmation of the new compounds was achieved using IR, Mass, H1, and C13 NMR spectroscopy techniques. In-silico molecular docking studies were conducted to identify the binding affinity of the synthesized compounds to the COX-2 enzyme (PDB ID: 3LN1), using PyRx software. The compounds were evaluated for in-vitro and in-vivo anti-inflammatory and antioxidant activities. For the in-vitro anti-inflammatory study, Cayman’s COX (ovine) colorimetric inhibitor screening assay kit was utilized, while for the in-vivo anti-inflammatory activity, the carrageenan-induced rat paw edema method was employed. The in-vitro antioxidant activity of the new derivatives was determined using the DPPH assay method with ascorbic acid as a standard drug. Regarding docking, most compounds exhibited good binding affinity, equal to or greater than that of the standard. All derivatives demonstrated moderate to good anti-inflammatory activity both in-vitro and in-vivo. They showed mild to moderate antioxidant activity compared to ascorbic acid. In conclusion,...
Read More
