STUDY OF FORCED DEGRADATION BEHAVIOUR OF A NOVEL PROTEASOME-INHIBITING ANTICANCER DRUG BY LC-MS AND DEVELOPMENT OF A VALIDATED STABILITY-INDICATING ASSAY METHOD
In the present study, comprehensive stress testing of Carfilzomib, a newly approved proteasome-inhibiting anticancer drug was carried out according to ICH guideline Q1A (R2). The drug was subjected to acid (0.1N HCl), neutral and alkaline (0.1N NaOH) hydrolytic conditions at 70 ºC, as well as to oxidative decomposition at room temperature. Photolysis was carried out in 0.1N HCl, water and 0.1N Na...
PROTECTIVE EFFECT OF BAUHINIA TOMENTOSA L. EXTRACT AGAINST GENTAMICIN INDUCED NEPHROTOXICITY IN WISTAR MALE ALBINO RATS
The aim of the present study was to explore the effect of aerial parts of Bauhinia tomentosa extract against gentamicin-induced nephrotoxicity. Thirty male Wistar albino rats were divided into five groups of six animals each. Animals served as Group I (normal control 5 ml/kg b.wt, p.o), Group II (toxic control), and Group III and IV (treatment groups 200 and 400 mg/kg b.wt, p.o) and Group V (stand...
The objective of the present investigation was to develop a sustained release (SR) tablets of Doxazosin Mesylate by wet granulation technique using a combination of synthetic polymers (Eudragit RS-100 and Eudragit RL-100). Different batches of Doxazosin Mesylate sustained release tablets were prepared by using microcrystalline cellulose as diluents by wet granulation technique. The compatibility o...
Non-nucleoside reverse transcriptase inhibitors (NNRTI) are a group of small hydrophobic compounds with diverse structure that specifically inhibit HIV-1 reverse transcriptase (RT) by allosteric bind to change its conformation. NNRTIs interact with HIV-1 RT by binding to a single site on the p66 subunit of the p66/p51 heterodimeric enzyme, termed the NNRTI-binding pocket (NNRTI-BP), binding intera...
PARENTERAL ADMINISTERED SUSTAINED RELEASE PIPERINE MICROPARTICLES INTENDED FOR TREATMENT OF LIVER FIBROSIS
The objective of this work was to formulate and characterize microparticles containing piperine, and evaluate their activity against carbon tetrachloride (CCl4)-induced liver toxicity. Piperine microparticles were formulated by o/w emulsion solvent evaporation technique using poly-ε-caprolactone as a polymer. Four different microparticle formulations (PM1, PM2, PM3, and PM4) were prepared by vary...
GROWTH INHIBITORY EFFECT ON BACTERIA OF SWIETENIA MACROPHYLLA KING SEEDS AND LEAVES CRUDE ALKALOID EXTRACTS
The uses of plant-based products for disease prevention and treatment become increasingly popular in many societies. Plants are considered one of the major sources for many bioactive groups such as alkaloids. The purpose of this study was to determine the rate of alkaloids and investigate antibacterial activity of alkaloid fraction of seeds and leaves from plant, genus Swietenia of S. macrophylla ...
DOSE DEPENDENT ANTIDIABETIC ACTIVITY OF HYDRO-METHANOLIC EXTRACT (3:2) OF FLOWER OF M. BALBISIANA IN STREPTOZOTOCIN INDUCED DIABETIC MALE RAT
Background: Musa balbisiana Colla (Musaceae family) is used as folkloric medicine throughout India. Objective: To search out the potent dose of a hydro-methanolic extract of Musa balbisiana flower for antidiabetic and antioxidative activities in streptozotocin-induced diabetic rat. Material and Methods: The extract of the said plant part was administered orally at the dose of 5, 10 or 20 mg /100 g...
RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF LOPINAVIR AND RITONAVIR IN COMBINED TABLET DOSAGEFORM AND IN SPIKED HUMAN PLASMA
A simple, accurate RP-HPLC method has been developed for simultaneous determination of Lopinavir (LPV) and Ritonavir (RTV) from tablet dosage form on reversed phase Brownlee C18 column. The sample was analyzed using acetonitrile: 10 mM ammonium acetate buffer (pH 4.5): Methanol (40:30:30) as a mobile phase at flow rate of 1.0 ml/min and detection at 210 nm using UV detector. The limit of detection...
BIOCHEMICAL STUDY OF AN ENDANGERED ETHNOMEDICINAL PLANT CURCULIGO ORCHIOIDES GAERTN. OCCURRING IN PURULIA DISTRICT OF WEST BENGAL, INDIA
Ethnic peoples from Purulia district of West Bengal in India use a lot of plants for their primary health care without studying phytochemical analysis and biological activities in detail. Main objectives of the present study are to evaluate the antibacterial and antioxidant activities of the whole plant extract of Curculigo orchioides using polar and nonpolar solvents. Total phenol content (TPC) w...
EVALUATION OF BONE MARROW FOR HEMATOLOGICAL ANOMALIES IN INDIAN PEDIATRIC AND ADULT PATIENTS: A SINGLE TERTIARY CARE CENTER STUDY
Bone marrow aspiration can determine the metastatic deposits, degree of cellularity and fibrosis readily. A retrospective analysis of bone marrow aspiration and biopsy, in children and adult patients with hematological anomalies, were performed in 674 cases for different indication of hematological abnormalities from January 2013 to March 2014. Cases were analyzed in detail regarding clinical, hem...