Increasing use of bioinformatics made very easy to researchers in biological and drug discovery field. Bioinformatics is involved at each and every step of drug discovery to drug development. The first step of the drug discovery is a question about disease and the treatment by drugs and whenever term drug comes, means something has to be given and the second question arises the given compound is d...
A RP-HPLC method was developed for estimation of rosuvastatin calcium and validated as per ICH guidelines. The estimation was carried out on C-18 Reliant (4.6 × 250 mm, 5 µm) column using a filtered and degassed mixture of acetonitrile: phosphate buffer pH 2.6 (in the ratio 70:30) as the mobile phase at a flow rate of 1.0 ml/min. The detection was carried out at wavelength of 241 nm. The method ...
A SIMPLE AND SENSITIVE RP-HPLC METHOD FOR TRACE-LEVEL QUANTIFICATION OF METHYL VINYL KETONE, A POTENTIAL GENOTOXIC IMPURITY, IN DRUG SUBSTANCES
An HPLC method was developed with the aim of quantifying the levels of Methyl Vinyl Ketone (MVK), a Potential Genotoxic Impurity (PGI), in Ivabradine HCl. A limit level concentration of 0.5 ppm for MVK was calculated by applying the concept of Threshold of Toxicological Concern (TTC). The developed HPLC method employs a Phenyl analytical column (150 mm х 4.6 mm, 3.5 μ), 5-μL injection volume, m...
The synthesis of nanoparticles has become the matter of great interest in recent times due to its various advantageous properties and applications in various fields. Though physical and chemical methods are more popular for nanoparticle synthesis, the biogenic production is a better option due to eco-friendliness. Morinda coreia leaf ethanol extracts was screened for the synthesis of Silver nanopa...
A NOVEL RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DAPAGLIFLOZIN AND SAXAGLIPTIN IN BULK AND PHARMACEUTICAL DOSAGE FORM
In the present work, a rapid, specific, accurate and precise Reversed phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Dapagliflozin and Saxagliptin in bulk and pharmaceutical dosage form. Successful chromatographic separation of Dapagliflozin and Saxagliptin was carried out with Inertsil-ODS, C18 column (250 × 4.6 m...
Objective: The purpose of the study was to evaluate the effect of an Ayurvedic formulation Varanadi kashayam to inhibit pancreatic lipase activity in-vitro. It also focuses on antioxidant activity, total phenol, flavonoid and phenolic acid content of fractions of Varanadi kashayam and HPTLC fingerprinting of fractions. Methods: Fractionation of Varanadi kashayam was done by using five solvents of ...
A STUDY ON THE ANTIMICROBIAL PROPERTY OF THE COTTON FABRIC IMPARTED WITH MICHAELIA CHAMPACA LEAF EXTRACT LOADED NANOPARTICLES
In the present work ethanol, methanol, ethyl acetate, chloroform and benzene extract of the Michaelia champaca leaves were screened for their antimicrobial activity. The ethanolic extracts of M. champaca leaves were proved to have the maximum antimicrobial activity; thus the ethanolic leaf extract was selected and the nanoparticles were synthesized using ionic gelification method. The nanopartic...
The aim of the study is to evaluate the diagnostic DNA profiles of the assessment of semen analysis data with respect to male fertility potential. Seman analysis taken form 50 patients of known fertility and 50 suspended infertility were studied by conventional semen analysis by suitable methods. The acridine orange test was used to assess the integrity of sperm DNA. The data throw doubt upon the ...
MATHEMATICALLY DESIGNED BIOPROCESS FOR RELEASE OF VALUE ADDED PRODUCTS WITH PHARMACEUTICAL APPLICATIONS FROM WASTES GENERATED FROM SPICES INDUSTRIES
A mathematical model optimizing bio-physico-chemical pre-treatment of steam exploded Piper nigrum, and Syzygium aromaticum lignocellulosic biomass waste was developed. The model was developed using, RSM, involving central composite face-centered design (CCD) with six parameters, three levels and 40 runs, at 95% confidence level. Six parameters optimized were quantity of each enzyme (a) Cellulase (...
Danio rerio is one of the best model organisms to study various kinds of disorders. Their genome is fully sequenced and they possess easily detectable developmental properties. The development of their embryo is quite rapid. This is important as the embryos are formed by external fertilization, are large and transparent. Abortifacient is a substance that induces abortion. It can be used in the for...