Posted by admin on Dec 1, 2014 in |
The synthesis of nanoparticles has become the matter of great interest in recent times due to its various advantageous properties and applications in various fields. Though physical and chemical methods are more popular for nanoparticle synthesis, the biogenic production is a better option due to eco-friendliness. Morinda coreia leaf ethanol extracts was screened for the synthesis of Silver nanoparticle using 0.1mM concentration of silver nitrate. The peak of 482 nm in UV-Vis Spectroscopy confirmed the presence of silver nanoparticle synthesis. Biosynthesized silver nanoparticles characterized by Scanning Electron Microscope (SEM), Fourier Transform Infra Red Spectroscopy (FT-IR) and Energy-dispersive X-ray spectroscopy (EDX). The silver nanoparticles around 90 nm were formed. Biologically synthesized silver nanoparticles were further examined for antimicrobial activity against both Gram-negative and Gram-positive bacteria. It was observed that a clear zone of growth inhibition was against in E. coli, Vibrio cholerae, Staphylococcus aureus, Bacillus sps, Pseudomonas aeruginosa, Klebsiella pneumoniae, Serratia marcescens, Proteus vulgaris, Staphylococcus mutans and Salmonella typhi confirms the antibacterial property of biologically synthesized...
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Posted by admin on Dec 1, 2014 in |
Metallothionein (MT) is a cysteine-rich protein that binds to heavy metals, and it is induced by them. The objective of this study to identify the MT protein induction/expression and localization in liver and kidney tissues (treated with 20 mg/L of CdCl2 for 72 hours) by immunohistochemical techniques and Western Blotting methods in the fresh water catfish, Clarias gariepinus. MT expression (MT-I and MT-II) levels were analyzed by Western blot method (using Mouse Monoclonal Anti-Metallothionein primary and Horseradish Peroxidase (HRP) secondary antibody). MT bands were detected using enhanced chemiluminescence system (West Femto Super Signal detection kit, Thermo Scientific Inc. USA) method. Antibodies showed a positive cross reactivity with MT proteins, MT immune activity was high in the cytoplasm of hepatocytes in Cd exposed fish liver and also detected in the nephrocytes present in the proximal tubules of the nephron and more precisely in their basal labyrinth in kidney. Western blotting showed increased MT expression levels in Cd treated tissues when compared with control tissue. The differential induction/localization of MTs in...
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Posted by admin on Dec 1, 2014 in |
The study undertaken for trace metal accumulation by fly ash, coal and mines waste in Singrauli industrialized district of Madhya Pradesh, India, using atomic absorption spectrometry. Present studies were focused into three Industrial, semi-urban and rural areas. Mostly sources of trace metals to plants are the air or soil from which these elements are taken up by foliage or root. Soil grown vegetable samples were collected from these distinct areas and were analyzed for Pb, Ni, Co, Cu, Zn and Cd concentration and their accumulation pattern. Only rural area is free from most of the contaminant and treated as control intended for these trace metals. From each studies area composite samples of common growing and consuming vegetables samples were analyzed quantitatively. In this investigation six commonly consumable vegetables Potato (Solanum Tuberosam), Tomato (Lycopersicon esculentum Mill), Karela (Momordica charantia) Lady’s finger (Abelmoschus esculentus Linn.), Brinjal (Solanum Melongena) Cabbage (Brassica oleracea) were studies for monitoring or observation of current status of following trace metals. Results indicated that an alarming situation in...
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Posted by admin on Dec 1, 2014 in |
Diclofenac (DIC) is a widely used as an anti-inflammatory drug and it is used as the toxicant in hepatoprotective studies. In the present study, hepato-renal protective activity of Colchicine against DIC induced liver and kidney injury in albino rats was assessed. Animals were injected with Diclofenac sodium at the single dose of 150 mg/ kg body weight daily for 28 days. Oral administration of colchicine at a concentration of 10µg/kg b.w daily for 28 days showed a significant decrease in plasma AST, ALT and creatinine as well as hepatic and renal thiobarbituric acid reactive substances and hydroperoxides. The treatment also resulted in a significant increase in GSH, SOD, CAT and GPx in the liver and kidney of DIC treated rats. The results clearly suggest that the colchicine treated group may effectively normalize the impaired antioxidant status in DIC- induced liver and kidney injury than the vitamin C-treated groups. However, Colchicine rapid protective effects against lipid peroxidation by scavenging of free radicals reduce the risk of oxidative...
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Posted by admin on Dec 1, 2014 in |
The combination of Levodopa and Carbidopa is widely prescribed for Parkinson’s disease. Levodopa easily crosses the blood-brain barrier and presumably converted to dopamine in the brain. Carbidopa inhibits decarboxylation of peripheral Levodopa but not of Levodopa within the central nervous system as it does not cross the blood-brain barrier. The purpose of present work was to develop bilayer floating dosage form of Carbidopa and Levodopa to improve the delivery of the drug at optimal absorption site and to reduce the frequency of administration with improved patient compliance. Bilayer floating tablet were prepared by using Kollidon SR and hydroxypropyl methylcellulose (HPMC K15M) as matrixing agents and sodium bicarbonate as gas generating agent. The IR spectral studies and DSC thermogram showed no interaction between drug and other additives. Experimental design was used for optimizing the ratio of Kollidon SR: HPMC K15 (X1) and concentration of binder (X2). The optimized formulation (batch F3) showed zero order drug release with Non-Fickian or anomalous diffusion. The optimized formulation showed similarity in dissolution with theoretical...
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