Posted by admin on Nov 1, 2014 in |
A simple, rapid and precise reverse phase high performance liquid chromatographic method has been developed for simultaneous estimation of Cinnarizine and Dimenhydrinate in Tablet Dosage Form. Chromatography was performed on a PhenomenexPartisil ODS C18 (250 X 4.6 mm) 5mm column using Acetonitrile: Water (90:10) as a mobile phase. The detection of the synthetic mixture was carried out at 265 nm with a flow rate of 0.7 ml/min. The retention times were 4.3 and 5.8 minutes for Cinnarizine and Dimenhydrinate, respectively. Proposed method was validated as per ICH guidelines for linearity, accuracy, precision; specificity and robustness for estimation of Cinnarizine and Dimenhydrinate in Tablet Dosage Form and results were found to be satisfactory. The linearity of the method was excellent over a concentration range 20-100μg/ml for Cinnarizine and 40-200μg/ml for Dimenhydrinate. The correlation coefficient was 0.996 and 0.992 for Cinnarizine and Dimenhydrinate, respectively. The limit of detection was 1.62μg/mL and 2.62μg/mL for Cinnarizine and Dimenhydrinate, respectively. The limit of quantitation was 4.93μg/mL and 7.95μg/ml for Cinnarizine and Dimenhydrinate, respectively. The...
Read More
Posted by admin on Nov 1, 2014 in |
The author selected and developed a new simple, sensitive and reproducible spectrofluorimetric method and validated for the determination of Memantine Hydrochloride in bulk as well as pharmaceutical formulations depending on condensation of Memantine hydrochloride with 1, 2 – Naphthoquinone -4- sulphonate (NQS) in an alkaline medium to become a product with orange color. The spectrofluorimetric method involved reduction of the product with potassium borohydrate and the later measurements of fluorescent formed lessen the NQS product after excitation at 295 nm and emission wavelength at 385 nm for the spectrofluorimetric method under optimum conditions, linear relationship with best correlation coefficient 0.9999 and the linearity was detected in between the range of 0.25-1.25 µg/mL. The mean accuracy was found to be 99.57 % to 99.66 %. The intraday and interday precision was found to be 0.14-0.16 % and 0.13-0.15% respectively. The limit of detection was found to be 0.0090µg/mL and the limit of quantification was 0.0270µg/mL. Therefore this newly recommended spectrofluorimetric method is most suitable for estimation of Memantine and observed...
Read More
Posted by admin on Nov 1, 2014 in |
Averrhoa carambola -L. (Oxalidaceae) has great importance in the Indian traditional system of medicine for controlling diabetes and piles: the combined extracts of leaves as well as fruits of A. carambola are used for the treatment of jaundice, gastric problems and other chronic conditions as well as in food supplements.The objective underlying this experiment is to determine the safety profile of the plant used on a daily basis as well as for prolonged exposure to subjects. In this study, we analyzed the influence of ethanol extract of Averrhoa carambola at a dose level of 2000 mg kg-1 bw on the acute and with a graded dose levels from 250, 500, 1000 and 2000 mg kg-1 bw for 28-day repeated-dose toxicity test in Wistar rats.The analysis revealed that the repeated oral dose of Averrhoa carambola L. at a dose level upto 2000 mg kg-1 bw for 28 days did not induce any biochemical, hematological, morphological, histopathological signs of toxicity. High dose of Averrhoa carambola L. extract did not inhibit the...
Read More
Posted by admin on Nov 1, 2014 in |
Electrochemical behavior of rupatadine, an anti-allergic drug was studied at graphene modified glassy carbon electrode in Britton-Robinson buffer (pH 6.5) by square wave voltammetry (frequency, 50 Hz; amplitude, 0.02 V; step potential, 0.005 V) and cyclic voltammetry (scan rates, 20-100 mV/s) techniques. Cyclic voltammetry revealed the oxidation of rupatadine corresponding to N-dealkylation which is the major metabolic pathway of rupatadine in the human body. Atomic force microscopy, scanning electron microscopy and electrochemical impedance spectroscopy was used to study the surface characteristics of the fabricated sensor. The electrode dynamic parameters were investigated and the oxidation mechanism was proposed. The lower limit of detection and quantification were achieved with the modified sensor as compared to the bare sensor due to enhancement in electron transfer process at the surface of modified sensor owing to greater electroactive surface area. The limit of detection was found to be 56.78 ng/ml and limit of quantification, 172.06 ng/ml at graphene modified glassy carbon electrode....
Read More
Posted by admin on Nov 1, 2014 in |
Etodolac is an anti-inflammatory drug that is poorly soluble in water. This paper describes an approach to improve the dissolution rate of Etodolac by using solid dispersions (SDs) in hydrophilic polymers. The solid dispersions prepared with a Co-evaporation, kneaded method & Physical Mixture method using different concentrations of α-cyclodextrin (α-CD). The release of Etodolac from various solid dispersions was determined from dissolution studies by use of USP dissolution apparatus. The dissolution study results revealed that there was a considerable increase in solubility of all solid dispersions as compared to pure drug. Prepared solid dispersions were characterized by DSC, PXRD, IR and SEM images were evaluated for drug content, saturation solubility. Physicochemical characterization of solid dispersions suggests a reduction in drug crystallinity following dissolution enhancement. Results indicate that present % DE 30 of drug was improved from 36.01 to 58.57 by the use of Etodolac α-CD-HPMC (1:2:0.3) Kneaded...
Read More
