Posted by admin on Sep 30, 2025 in |
Overexpression of Matrix metalloproteinases (MMPs) in cancers promotes tumor by degrading the barrier of extracellular matrix and angiogenesis. MMP-9 plays a major role in the progression of cancers by enhancing migration, cell survival, angiogenesis, epithelial-to-mesenchymal transition, immune response induction, and tumor microenvironment formation thus, it is considered as a lead drug target to treat cancer. NSAIDs or Nonsteroidal anti-inflammatory drugs have been widely investigated for their effectiveness in different cancer types but promising candidates for clinical use are still halfway. The present study aims to investigate a combination of NSAIDs that can convey high-potential structural inhibition against MMP-9 utilizing a molecular docking-based in-silico approach. A thorough literature survey followed by ADMET analysis provided the best NSAID candidates covering diverse chemical space for the analysis. Molecular docking of NSAIDs with MMP-9 individually proved oxaprozin and piroxicam as best candidates for structural inhibition of MMP-9. Combination docking gave a high binding energy of -12.98 kcal/mol for the synergistic inhibitory effect of oxaprozin and piroxicam against MMP-9. Thus, further in-vitro analysis can...
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Posted by admin on Sep 30, 2025 in |
Objective: The purpose of this study was to compare the impact of various drying methods, such as sun drying, Sunshade drying, hot air oven drying, and Deep freeze-drying technologies, on the elucidation of sensory and nutritional profiles in Sauropus androgynous leaves. Methods: Fresh Sauropus androgynous leaves were used for various drying techniques, such as Sunshade, sun drying, hot air oven, and Deep freeze drying. The leaves were analyzed for changes in nutritional composition, mineral composition, and vitamin concentration. The study involved various drying analysis methods based on the AOAC protocols to evaluate their impact on the leaves’ nutritional and medicinal properties. Results: Sun-dried leaves retained a higher proportion of essential nutrients compared to those dried in a sun shade, hot air oven, or Deep freeze method. The sensory evaluation demonstrated that sun-dried leaves were preferred in color, taste, antioxidants, and overall acceptability compared to leaves dried in a hot air oven. Conclusion: Sun drying is the best technique for maintaining the sensory qualities, nutritional value, and antioxidants of Sauropus...
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Posted by admin on Aug 31, 2025 in |
Strobilanthes hamiltoniana (Paalkurinji), a perennial herb of the Acanthaceae family, has traditional use in treating ulcers, diabetes, arthritis, and wounds. S. hamiltoniana leaves were extracted using ethanol, chloroform, and water, and screened for phytochemicals using standard protocols. LC-MS analysis was used to identify flavonoids. Antioxidant activity was evaluated via DPPH, HRS, and FRAP assays, while anti-inflammatory activity was assessed using the NO scavenging assay. Additionally, DFT (B3LYP/6-311G(d,p)) calculations and molecular docking were performed to investigate interactions with antioxidant-related proteins: monoamine oxidase-B, catalase, cytochrome P450, and NFE2-related factor 2. Based on the DPPH assay, aqueous and chloroform extracts showed 25.74% and 45.94% inhibition, while ethanol and methanol extracts exhibited higher inhibition at 89.10% and 86%. HRS ranged from 25.5% to 86.4%, with ethanol extract showing the highest activity (86.4%) at 300 μg/ml and an IC₅₀ of 25.54 μg/ml. All extracts were effective in the FRAP assay, with ethanol showing the highest activity (79.41%, IC₅₀ = 28.24 μg/ml). In the NO scavenging assay, ethanol again showed the strongest activity (80.41%, IC₅₀ = 26.26 μg/ml). Molecular docking revealed catechin had...
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Posted by admin on Aug 31, 2025 in |
Introduction: Schizophrenia is a multi-factorial neuropsychiatric disorder, influenced by environmental and genetic factors. The catechol-O-methyltransferase (COMT) gene is a crucial for the metabolism of dopamine. It has been thoroughly investigated for its potential role in SCZ. At the same time; several variants in the COMT gene have been reported in diverse populations in South Karnataka but remain unexplored. Aim: To assess the novel variants related to the COMT gene and their correlation with SCZ symptoms and other health issues in patients. Materials and Methods: The study was carried out on 80 patients aged between 18 and 60 years; the results were compared with healthy controls of 80. The samples of blood were collected for the DNA extraction. PCR was used to amplify the COMT gene, followed by sequencing to identify variants. Standardized scales were used for gauging the severity of the symptoms. Results: Two novel changes in a single nucleotide (SNPs) (non-synonymous) were identified in the COMT gene in exon -4 of two female SCZ patients out of 80...
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Posted by admin on Aug 31, 2025 in |
Background: Drug Utilization Study offers useful methods for teaching and training in drug therapy. In our setting, there is no such study that describes the use of drugs post-operatively. As a result, this study was conducted to investigate the post-operative drug utilization pattern in indoor post-operative patients. Aim: To study the prescribing trends of drugs used in indoor adult post operative patients in general surgery unit. Methods: Prospective observational study was conducted in post operative patients in General Surgery wards and ICU for 12 months at a tertiary care teaching hospital. Results: Total 600 cases were studied. Majority of the patients managed were males (64.5%). Most of the patients were in 18-40 years age group (49.66%) and from rural background 69.33% (416). The most common surgical procedure performed was Appendicectomy (29%). A total of 4437 drug products were prescribed. Average number of drugs prescribed per encounter were 7.39±2.56. Only 41.08 % of the drugs were prescribed by Generic name. Antibiotics were prescribed to 99.66% of the encounters. Antibiotics per...
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