Posted by admin on Sep 1, 2012 in |
A simple, sensitive and reproducible method was developed and validated for the simultaneous estimation of Diethylcarbamazine and Levocetirizine in its tablet formulation by reverse phase high performance liquid chromatography using Waters1515 HPLC with Diethylcarbamazine and Levocetirizine simultaneously. HPLC-PDA detector at the λmax of 253nm, using Hypersil-BDS C18 (250×4.6 mm. 5 μ) column. The mobile phase used was potassium dihydrogen orthophosphate buffer (pH: 5): acetonitrile (20:80 v/v) with isocratic flow (flow rate 1 ml/min) and the pH was adjusted with orthophosphoric acid. The compounds Diethylcarbamazine, Levocetirizine were eluted at 2.04, 5.54 min, respectively. The peaks were eluted with better resolution. The method was accurate with assay values of 99.67% and 99.81% w/w, precise (%RSD) with 0.3and 0.5, percentage recovery values of pure drug were in between 99.4% to 100% and 99% to 99.4% which are very sensitive with limit of detections (LOD)’s 2.42 and 0.08ppm and limit of quantification (LOQ)’s 7.42 and 0.2ppm, these results are within the range of limits 98% to 101% which indicates that the method was...
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Posted by admin on Sep 1, 2012 in |
Aim of present investigation was to isolate natural mucoadhesive agent from Ocimum americanum seeds and characterize through in vitro mucoadhesion methods, FTIR and DSC studies. Mucoadhesion force of isolated natural mucoadhesive agents and synthetic polymer Carbopol 934P was determined using in vitro mucoadhesion methods viz. Wihelmy’s method, falling sphere method and modified physical balance method. The research study reveals that the natural mucoadhesive agent isolated from Ocimum americanum seed was shown promising mucoadhesion strength. The formation of hydrogen bond by natural mucoadhesive agent with mucosa was confirmed by FTIR spectra showing carboxyl and hydroxyl groups. Natural mucoadhesive agent may be useful to formulate mucoadhesive drug delivery systems as it bears excellent mucoadhesion property and advantageous over synthetic polymers for less toxicity, biocompatibility, biodegradability and...
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Posted by admin on Sep 1, 2012 in |
Paracetamol is a widely used non-prescription analgesic and antipyretic medicine. The study was conducted to assess the comparative in-vitro quality control parameters through the evaluation of weight variation, hardness, friability, disintegration time and dissolution profile between the commercially available tablet brands of paracetamol. Tablets of two manufacturers of the formulation were evaluated in two groups A and B. The similarities were found between both the groups. Both tablet brands of paracetamol (1.0 to 1.6%) showed acceptable weight variation and friability (below 1%). Both tablet brands were somewhat different in their hardness, disintegration time and dissolution profile. It can be concluded that standard quality control parameters always should be maintained not for paracetamol but also for all kinds of medicine for getting better drug...
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Posted by admin on Sep 1, 2012 in |
To analyse the situation of antibiotic resistance, a total of 77 E. coli isolates from urine, pus, sputum and endo-tracheal aspirate were screened for their antibiograms for antibiotic resistance, multiple antibiotic resistance (MAR) index for evaluating the spread of resistance and plasmid profiles for the presence and characterization of plasmids. Very high resistance level (>90%) was detected against ampicillin, amoxicillin, ceftazidime, norfloxacin, tetracycline while imipenem and amikacin recorded the least resistance levels of 2.3% and 13.9% respectively among the isolates. An increased resistance to amoxicillin, tetracycline, cotrimoxazole and norfloxacin were observed in this geographical area which however displayed a lower resistance in other countries. The MAR index varied considerably, the lowest was 0.18 and the highest was 0.89. Plasmids of 10 size ranges were detected in the isolates. Some isolates possessed single sized plasmid while other possessed multiple plasmids. Isolates with high multi- antibiotic resistance profiles were found to possess multiple plasmids. This study shows that regular antimicrobial sensitivity surveillance is necessary and acquisition of plasmid could greatly contribute...
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Posted by admin on Sep 1, 2012 in |
The methanol extract of Centella asiatica (Family:Umbelliferae) was studied for its phytochemical and pharmacological Activities. This study of the extracts indicates the presence ofReducing Sugar Tannins, Alkaloids, Sapponins and.The pharmacological study include Antimicrobial activities.The extract of the plant showed activity against a wide a variety of microorganism’s .All the activities were compared by measuring the zone of inhibition with the standard antibiotic. Based on the findings of Antimicrobial activity, we can say that the plant may contain novel compounds that possess potent anti-mutagenic activity and anti-oxidative activity. The obtained results support for the uses of this plant as traditional medicine. Infectious diseases are the second leading cause of death worldwide. Treatment of infections continues to be problematic in modern time because of the severe side effects of some drugs and the growing resistance to antimicrobial agents. The activities observed could be attributed to the presence of some of the phytochemicals detected which have been associated with antibacterial...
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