Posted by admin on Sep 30, 2020 in |
Varieties of natural products have been proved as a rich source of phytoconstituents which are reported for a wide range of therapeutic potential. Plumbago zeylanica L. Is one of the herbs which are traditionally used for its therapeutic value. It is reported for several bioactive chemical constituents like naphthoquinones, flavonoids, alkaloids, glycosides, steroids, tri-terpenoids, tannins, fixed oils, fats, proteins, etc. in its chemical composition. Plumbagin is one of the most bioactive compounds of Plumbago zeylanica L., which is reported for a wide range of pharmacological activities such as anti-cancer, anti-diabetic, anti-malarial, anti-microbial, etc. It is a naphthoquinone derived yellow crystalline phytochemical constituent which is well proven for cytotoxicity against various cancer cell lines both in-vitro and in-vivo. This study is aimed to review and highlight the anticancer potential of plumbagin, formulation development approaches, and issues in the dosage form design of Plumbago zeylanica L. and its isolated constituent...
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Posted by admin on Sep 30, 2020 in |
Linezolid loaded Solid Lipid Nanoparticles (SLN) were prepared and then incorporated in a topical gel as a carrier. The formulation was prepared by solvent injection method followed by probe sonication for reducing particle size. Excipients like lipids (Phospholipon 90H, Lipoid S-100, and Lipoid S-75) and emulsifier (Tween-80, Span-20, and poloxamer-407) were optimized using Taguchi L9 orthogonal design of an experiment. The optimized formulations were based on entrapment efficiency and were further evaluated for particle size, polydispersity index, zeta potential, in-vitro and ex-vivo studies. SLN4, SLN5, and SLN6 were optimized as their entrapment was more compared to other formulations. The results obtained were 93.05 nm of particle size, 0.252 is PDI, and -13.0 mV of zeta potential. From the in-vitro studies, it has been found that SLN5 with 2% of lipid is showing sustained-release followed by SLN4 (1.5%) and SLN6 (1%), and hence SLN5 was selected for evaluations like ex-vivo studies, and stability studies of gel. Ex-vivo permeation studies have shown sustained release of drugs with a flux of 11.22...
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Posted by admin on Sep 30, 2020 in |
This study designed to evaluate the antiplatelet and antihemolytic activity of hydroethanolic extract of Annona muricata leaves (HEEAM). The platelet aggregation assay was carried out using platelet-rich plasma, and the antihemolytic assay was done by hydrogen peroxide with different concentration of plant extract (100-500 μg/ml). HEEAM was found to possess more hydrogen donating ability when compared with the standard drug (aspirin). In addition, extract showed significant antihemolytic action (IC50 = 64.94 ± 0.01 µg/ml) against hydrogen peroxide (H2O2) induced hemolysis. It measures the degree of hemolysis when generated free radical damage. These were evident that the extracts exhibited strong antioxidant activity by protecting the red blood cells from hemolysis. Furthermore, the antiplatelet activity of HEEAM showed a maximum at 500 μg/ml concentration. It represents a promising treatment for thrombotic diseases. The percentage of hemolysis and platelet aggregation was calculated with standard aspirin. In conclusion, these observations suggest that HEEAM to posses a significant antiplatelet and antihemolytic...
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Posted by admin on Sep 30, 2020 in |
Cytochrome c oxidase, a mitochondrial metalloenzyme acting as the terminal enzyme of the mitochondrial respiratory chain. It provides a critical function in cellular respiration in both eukaryotes and prokaryotes. The recent development in technology has enabled researchers to understand the genetic, molecular, structural, and functional properties of proteins to identify appropriate targets against diseases, and as a result, many anti-inflammatory and anti-proliferative drugs have been developed. In the absence of X-ray and NMR protein crystal structure, homology modeling provides a useful 3D model for a protein that is related to at least one known protein structure. In the present study, the 3D molecular structures of nine Cytochrome c oxidase subunit 1 protein from different species such as Myxine glutinosa, Struthio camelus, Sus scrofa, Homo sapiens, Xenopus laevis, Halichoerus grypus, Zygogeomys trichopus, Buteo buteo, and Plasmodium falciparum were predicted using homology modeling software MODDLLER. The modeled structures were docked using Autodock4.2 software with ten different natural compounds and three drugs as a control to study the molecular interactions of these...
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Posted by admin on Sep 30, 2020 in |
The majority of the drugs that are available in the market which are intended for activity in the central nervous system, are not capable of being transported across the Blood-brain barrier. The nasal route of drug administration is one of the most promising routes of drug administration that can permeate the drug through BBB. The permeability of drugs across the BBB is depending on the physicochemical properties of the drugs. Drugs with molecular weight lesser than 600Daltons and partition coefficient (Log P) within the range of 1.5-2.7 might pass through the BBB. Nanocarriers are capable of getting drugs with higher molecular weight transported across the BBB. Incorporating the drug into these carriers can facilitate the transfer of drugs across BBB. SLNs and NLCs are the most prominent carrier of drugs that can be employed for the transfer of drugs to the CNS. The drugs, when administered through nasal route, are carried across the BBB mainly through two major pathways, they are olfactory pathway and trigeminal pathway. The main disadvantage...
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