Posted by admin on Mar 31, 2023 in |
Objectives: Cyclophosphamide is an alkylating agent known for its reproductive toxicity in males. This study evaluates the protective effects of Withania somnifera extract (WSE) on cyclophosphamide (CP) induced testicular toxicity and DNA damage in male rats. Methods: Adult male rats were randomly divided into four groups Group1-Control, Group 2- Cyclophosphamide, Group 3- Withania somnifera extract, Group4- Cyclophosphamide+ Withania somnifera. After the experimental period of 8 weeks, sperm quality, hormonal analysis, and DNA damage studies were carried out. Histopathological analysis of testes was performed for the assessment of spermatogenic disorders. Results: In CP-treated rats, sperm quality was reduced with decreased testosterone levels with increased DNA damage. Co-administration of WSE with CP improved sperm quality, testosterone levels, spermatogenesis and genotoxicity of cyclophosphamide. The histopathological study also restored most of the degenerative and necrotic changes. Conclusions: This study suggests a prominent role of WS against CP-induced testicular toxicity and DNA...
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Posted by admin on Mar 31, 2023 in |
Hyperlipidemia is an elevated condition of lipid levels in the body and is known to speed up a process of atherosclerosis that may prove fatal in the development of various cardiovascular diseases. The figures of the patient with hyperlipidemia are constantly increasing which is rising a serious risk factor for a group of cardiovascular diseases. Although various pharmacotherapy for hyperlipidemia namely statins, niacin, fibric acid derivatives, cholesterol absorption inhibitors, and bioactive are available but suffer from various hurdles such as inadequate solubility and poor absorption leading to low bioavailability and ineffectiveness in lowering of cholesterol levels only up maximum 40%. Hence it is required to combat these hurdles associated with hyperlipidemia. Nanotechnology has provided revolutionary changes in medicine, providing a novel approach to treating hyperlipidemia. Various nanotechnology-based approaches include nanosuspension, nanoemulsion, nanoparticle, polymeric nanoparticle, dendrimer, polymeric micelle, etc. out of which nanosuspension has provided a strong background to combat the hurdles linked with pharmacotherapy due to possession of various benefits such as improved aqueous solubility, enhanced bioavailability, fast onset...
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Posted by admin on Mar 31, 2023 in |
Neurodegenerative diseases (NDs) that are characterized by progressive loss of functioning and structure of neurons are of great burden to the individuals and the society. With several hypotheses, the existent cause still remains a mystery for various NDs in healthcare. Protein degradation, inflammation, oxidative stress, environmental factor, mitochondrial defects, abnormal protein accumulation in neuron and familial history are amongst the commonly studied environmental causative factors for NDs. These disorders are a significant cause of disability and mortality, and consequently increasing life spans is one of the key challenges for medical research. The excessive treatment cost has led the world to move to alternative therapy with lower cost and minimal side effects compared to conventional treatments. Medicinal plants have been used for treating various diseases since ancient times in India. Medicinal plants used in Unani System of Medicine cover most of the NDs. It has defined a huge number of plants with multiple therapeutic benefits. In this review, the role of important medicinal plants, viz., Prunella vulgaris L., Melissa officinalis...
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Posted by admin on Mar 31, 2023 in |
The present study investigated the effect of intake of aspartame in diabetic rats, a widely used artificial sweetener (aspartame), on antioxidant defence status in the rat brain. Adult male Wistar rats weighed 180 ± 20 gm were randomly divided into 4 groups as follows: The first group was control; the second group was given aspartame (ASP) at a dose of 50 mg/kg body weight; the third group was Diabetes (D) (Streptozotocin (STZ) 45 mg/kg body weight); and fourth group was Diabetes rats (STZ 45 mg/kg body weight) administered with ASP (D+ASP) (50 mg/kg body weight). Induction of Diabetes (STZ) through intraperitoneal injection at a single dose. ASP is administered with gavage for up to 30 days. Superoxide dismutase, catalase and glutathione reductase are significantly reduced. In contrast, in lipid peroxidation, a significant increase was observed in the various brain regions of the experimental group rats compared with the control group rats. The results indicate that consumption of aspartame leads to an imbalance in the normal and diabetic rats’ antioxidant/pro-oxidant...
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Posted by admin on Mar 31, 2022 in |
Remogliflozin Etabonate (RE) is the latest addition to the sodium-glucose transport proteins 2 inhibitor class of drugs recently approved in India to manage type 2 Diabetes Mellitus. Literature survey revealed that no high-performance thin-layer chromatographic (HPTLC) method had been reported to date for this drug. The present work describes the development and validation of an HPTLC method for RE. The chromatography was performed on pre-coated silica gel 60 F 254 plates using methanol: toluene: ethyl acetate (1:4:5) v/v/v as mobile phase. A thin layer chromatographic (TLC) scanner set at 228 nm was used to directly evaluate the chromatograms in reflectance/absorbance mode. The drug was satisfactorily resolved with Rf 0.45. The method was validated according to the International Council on Harmonization (ICH) guidelines. The calibration plot was linear between 50–300 ng/b and respectively. The accuracy and precision of the proposed method were evaluated by recovery studies and intra-day and inter-day precision studies, respectively. In stability testing, RE was found to be susceptible to alkaline degradation. Because the method could effectively...
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