Posted by admin on Mar 31, 2022 in |
Coconut oil is an edible vegetable oil used in the food, pharmaceuticals, and cosmetics industries. Fatty acids are the major constituents of Coconut oil. Various methods were reported for the extraction of oil from coconut seed kernels. Extraction of coconut oil from its seed kernel using the probe sonication method is not reported in the literature. Hence, in the present study, oil was extracted using hexane and ethyl acetate. The physicochemical parameters like acid value, saponification value, refractive index, specific gravity, unsaponifiable matter, moisture content and iodine value were determined for the extracted oil. Oil samples after base-catalyzed derivatization were analyzed for the quantification of fatty acids by the GC-FID method. The percentage of oil extracted from coconut seed kernel was 31.84% and 36.32% for n-hexane and ethyl acetate extractions, respectively. Physico-Chemical parameters of the oils tested were within limits. Coconut oil obtained from n-hexane extraction contained the highest fraction of lauric acid. Oil extraction with probe sonication technique was found to be efficient as the percentage recovery of...
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Posted by admin on Mar 31, 2022 in |
Mucormycosis is a new fungal disease that increased the morbidity and mortality rate in this present scenario. It’s difficult to diagnose and cure due to proper scientific evidence is not yet being designed. The disease’s prevalence appears to be rising. In high-income nations, hematological malignancies are the most prevalent underlying illness, while uncontrolled diabetes is usually linked to pulmonary mucormycosis in low-income nations. Mucormycetes can be detected or identified using histopathology and molecular tests, and they can be recommended as important add-on techniques to augment standard diagnostic techniques. Reversal of underlying predisposing factors, early delivery of active antifungal medications at the appropriate dosage, thorough excision of all diseased tissues and different adjuvant treatments are all part of a successful therapy strategy mucormycosis. Liposomal amphotericin B antifungal medications have been added to our antifungal arsenal, but amphotericin B remains the medication of choice for first antifungal therapy. Despite attempts to better understand the pathophysiology of mucormycosis, early diagnosis and intensive treatment, the disease’s fatality rate remains...
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Posted by admin on Mar 31, 2021 in |
The aim of this investigation was to re-evaluate the in-vitro mutagenic, genotoxic, and aneugenic potential of omeprazole through the Ames test, comet (SCGE), and cytokinesis-block micronucleus assays (CBMN), as previous reports on the mutagenic and genotoxic effects of omeprazole are scarce and inconclusive. The results of this study indicated a negative mutagenic effect through the Ames test. Comet assay endpoints such as tail length, tail intensity, and tail moment showed a mild genotoxic effect with dose-dependence. The CBMN assay endpoints were implemented according to the requirements for in-vitro evaluation of genotoxicity after 4 h of exposure, with or without metabolic activation. Endpoints, such as binucleated cells with micronuclei (BNMN), total micronuclei (MN), arrested metaphasis (AM), nuclear buds (NB), nucleoplasmic bridges (NPB), and necrotic cells (NC), showed a dose-dependent effect. The number of apoptotic cells (AP) showed a statistically significant increase compare to the control value but was without dose-dependence. This in-vitro study suggests omeprazole exhibits mild genotoxic and aneugenic...
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Posted by admin on Mar 31, 2021 in |
Three simple and economical UV-spectrophotometric methods have been developed and validated for simultaneous estimation of ciprofloxacin (CIP) and metronidazole (MET) in a tablet dosage form using distilled water as a green solvent. The proposed methods were; simultaneous equation method (method A), Q-absorbance ratio method (method B), and area under curve method (method C). λmax of CIP & MET in distilled water were found to be 271 nm and 320 nm, respectively. The isoabsorptive point was observed at 290 nm. The linearity was obtained in the concentration range of 1-9 μg/ml, and 2-18 μg/ml for CIP and MET respectively by methods A, B & C. Validation parameters were carried out. All three methods were found to be linear, accurate, precise, and specific. Good results were achieved using distilled water as solvent due to its greater solubility, reproducible readings with maximum absorbance. Among the three methods, method C was found to be the most sensitive. Hence, this method can be recommended for the routine analysis of this drug...
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Posted by admin on Mar 31, 2021 in |
Hypertension is now defined on the basis of systolic (SBP) and diastolic (DBP) blood pressure levels and classified into stages on the basis of the degree of elevation. The generally recognized cut point for hypertension is an average office BP of 140/90 mmHg or greater, which has been obtained by a recommended standard technique with an accurate manometer and has been confirmed on at least one other occasion. Hypertension is the main cause of high blood pressure (BP), and BP is a major public health issue in India and is rampant among both urban and rural populations. It is the key modifiable risk factor for many diseases. Prescription-event monitoring or PEM involves a methodology in which the safety of new medicines are studied that are used by general practitioners (GPs). PEM is an observational cohort form of pharmacovigilance that is non-interventional, in the sense that nothing happens to interfere with the doctor’s decision regarding which drug to prescribe for each individual patient. In this study, we focused on the...
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