Posted by admin on Jun 30, 2021 in |
Phytotherapeutical properties of blackthorn (Prunus spinosa L.) are well known and used in Bosnia and Herzegovina traditional medicine. This study aimed to investigate total phenolic content, total anthocyanin content, total flavonoid content, antioxidant and antibacterial activities of different ethanolic extracts from wild blackthorn flowers, leaves and fruits, obtained by microwave-assisted extraction and ultrasound-assisted maceration. The mineral profile has been identified using flame atomic absorption spectrophotometry. Plant samples were collected from three areas in Bosnia and Herzegovina. In order to detect antioxidant activity of extracts, DPPH assay was carried out. DPPH radical scavenging activity was higher for flower and fruit extract than leaf extracts, regardless of the extraction method used. Ethanolic extracts show significant antimicrobial activity against all analyzed bacterial strains (Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis, Salmonella enterica, Escherichia coli, Pseudomonas aeruginosa and antifungal activity against Candida albicans). Blackthorn fruits contained high mass fractions of some essential minerals on dry mass basis, including K, Ca, Mg, Na, Fe, and Cu. Blackthorn flower, leaf and fruit extracts exhibit a high...
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Posted by admin on Jun 30, 2021 in |
Several spectrophotometric and HPLC methods for the determination of lornoxicam and eperisone hydrochloride have been documented individually or in combination with other drugs in pharmaceutical dosage forms. Therefore, in the present study, a reasonable and practical reverse phase HPLC method was developed and validated for lornoxicam and eperisone hydro-chloride in bulk formulation. In RP-HPLC method, the analyte was resolved by using an isocratic system, Methanol: Water (55:45) with 0.1% v/v Ammonium Hydroxide, pH 7.4 was used as mobile phase, at a flow rate of 1.0 ml/min, on HPLC system containing C18 analytical column (25 × 0.46 cm, 5 µm). The detection was carried out at 274 nm. The retention time was found to be 7.4 min and 9.2 min for lornoxicam and eperisone hydrochloride, respectively. Good resolution and retention time were observed. The results of the analysis in the method were validated with respect to following parameters linearity, precision, specificity and system suitability, limit of detection and limit of quantification....
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Posted by admin on Jun 30, 2021 in |
Considering the increasing trend of the cancer rate of numerous organs, efficient treatments are highly required to restrain human melanomas. Cancer is among the most prevalent illnesses ascertained in developed countries, estimating more than 1.50 million fresh instances in the US alone in 2017. Though, approximately most of the chemotherapeutic drugs presently on the market cause major adverse effects like immunodeficiency, fatigue, alopecia, anaemia, peripheral neuropathy, neurological problems, and fertility issues, to name a few of them. Polysaccharides have developed as potential chemical entities revealing effective immunomodulatory activity within a variety of cell lines and can be developed as alternatives to existing cancer chemotherapeutic agents, possessing minimal toxic side-effects. Polysaccharides isolated from plants, microorganisms, fungi, and marine sources have been reported. They function via TLR modulation, activation and maturation of Dendritic cells, Cytokines signalling pathway, macrophage activation, and miscellaneous pathways like PI3K, MAPK activation, the complement supplement system. This review concentrates on the polysaccharides explored over the past five to seven years as an adjuvant therapeutic agent, their suggested...
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Posted by admin on Jun 30, 2021 in |
Bacterial infections are one of the prominent causes of health problems and mortalities around the world. Medicinal plants are a rich source of antimicrobial agents and provide a safer and cost-effective way of treating bacterial infections. The objective of the present investigation was to evaluate the anti-bacterial potential of petroleum ether and methanol extracts of Anacardium occidentale L. leaf. The anti-bacterial potential of A. occidentale L. leaf was tested against gastrointestinal tract infection-causing bacteria such as Escherichia coli, Shigella flexneri, Enterococcus faecalis, Vibrio cholariae, Staphylococcus aureus, Klebsiella pneumoniae, and Salmonella typhi by using the agar well diffusion method. Streptomycin was taken as the reference antibiotic. The results indicated that petroleum ether extract of A. occidentale L. exhibited the highest zone of inhibition against Shigella flexneri (26±0.75) and least against Staphylococcus aureus (11±1.32). In contrast, methanolic extract exhibited the highest zone of inhibition on Vibrio cholariae (20±1.41) and least against Escherichia coli (11±0.21). The preliminary phytochemical analysis revealed the presence of alkaloids, glycosides, tannins and polyphenolic compounds, flavonoids, saponins, steroids,...
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Posted by admin on Jun 30, 2021 in |
COVID-19 has been proved to create a pandemic over the world within a few years. All scientists and health workers are trying to develop an effective treatment with collaboration. Various strategies such as antiviral, antibacterial, and antimalarial drugs have been employed to treat COVID- 19, but they were found to relieve the symptoms only. The formation of various variants of the virus in various global parts made the treatment of COVID-19 more difficult. Developing an effective vaccine is the main goal of clinicians and scientists, which is under clinical trials, and some vaccines which have been approved for the treatment such as COVAXIN, COVISHIELD, ASTRA Zenaca, and SPUTNIK V vaccines are being used to develop antibodies against COVID-19, but due to spread of variants of virus these vaccines are not supposed to be 100 percent protective against the virus. With the help of nanotechnology, the treatment of SARS- CoV-2 can be made efficient due to its direct target on viral cells and modifying its genetic material functionings. In this...
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