Posted by admin on Jul 31, 2019 in |
Angiogenesis is the formation of new blood vessels from preexisting vasculature. It is a key process in some physiological conditions such as wound healing, growth and reproduction. Any disturbance in mechanisms of angiogenesis plays a key role in the pathogenesis of some diseases through the over-proliferation of blood vessels such as cancers, psoriasis, arthritis, retinopathies, obesity, asthma, and atherosclerosis and cardiovascular diseases, which have the highest rates of morbidity and mortality worldwide. Anti-angiogenic therapy has been proved to be a potential cure in the past decades for a number of cancers. Still, antiangiogenic therapy has to be realized to its fullest potential to turn cancer into a dormant disease. Many angiogenic inhibitors have been developed till date, and the main problem is the multiple angiogenic molecules produced by tumors and tumors at different stages of development. Therefore, blocking a single angiogenic molecule has less effect or no effect. Selection of a suitable model for angiogenesis research remains a challenge. Thus, in recent years, several models for evaluation of angiogenesis...
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Posted by admin on Jul 31, 2019 in |
Single-walled carbon nanotubes (SWNTs) though emerged as a promising material for delivery of biomolecules into various cells, due to their high cytotoxicity, they are limited in use in many biological systems and also in humans. The present research explores the preparation of functionalized SWNTs of low cytotoxicity and biocompatibility by altering the size and surface functionalization. Noncovalent amine functionalization improves their biocompatibility, solubility and alters their cellular interaction pathways resulting in reduced cytotoxic effects so that the biomolecule will be preferably released from the carbon nanotube when the complex has been taken up by endocytosis into the cells. The noncovalent functionalization of SWNTs was carried out by using polymer 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino (polyethylene glycol) – 2000] (ammonium salt) (DSPE-PEG 2000 amine) which makes the hydrophobic lipid chains of PEG to get strongly anchored onto the nanotube surface, whereas the hydrophilic chain renders SWNTs water soluble and biocompatible which results in the development of NH2-PEG-SWNTs. The functionalized SWNTs were further characterized by FE-SEM, Raman spectroscopy, FT-IR and UV-VIS-NIR, and zeta potential. So...
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Posted by admin on Jul 31, 2019 in |
Klebsiella pneumoniae is a significant pathogen for nosocomial infections and quickly becomes the most prevalent beta-lactamases produced that are resistant to several antimicrobials. To comprehend deeper the condition of multidrug resistance [MDR]. Objectives: Our research was directed at identifying Klebsiella pneumoniae isolates containing shv β-lactamase gene and the relationship between them and some antimicrobial resistance in order to prevent rejection of therapy. Methods: Overall, 64 K. pneumonia it gathered pneumonia isolates and conducted a drug susceptibility test for 13 different antibiotics by the system Vitek 2 to identify their susceptibility patterns. Phenotypic tests for species producing ß-lactamase have determined the resistance phenotype of isolates. Results: The information indicated that 60[93.7%] of the entire isolates tested were Shv genes, while the remainder were positive. The PCR was used to amplified drug resistance. Highest antibiotic resistance in instances of ESBL positive. In fact, 2[33.3%] of the phenotypical confirmatory Test [PCT] sample were shown to be negative isolates. The data has shown that prediagnosed positive phénotype isolates were genetically positive for EsBL development. Concept: the...
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Posted by admin on Jul 31, 2019 in |
Several plants across the world possess specific therapeutic and diagnostic properties. The identification of the properties among them is the most difficult task. Several researchers have used several methodologies to express the therapeutic properties in the plants. The interest of scientists among the anticancer studies has been increasing widely as cancer has become one of the deadliest diseases. The usage of different parts of the plant and using the different extracting solvents has shown effective results on different types of cancer cell lines. Several plants synthesize metabolites which possess anticancer properties that have been used in the development of drugs by clinical trials. The application of nanoparticles from plants in cancer treatment has been regarded as a successful method recently. The present review aims at the study of a broad spectrum of plants having anticancer properties using the in-vitro analysis of particular cell lines. The anticancer activity was analyzed by different assays such as MTT assay, “Alamar Blue” Resazurin reduction assay, SRB assay, and WST-1 assay. The results were...
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Posted by admin on Jul 31, 2019 in |
Tectaria macrodonta C. Chr. locally known as Kaali negro in Sikkim is a rhizome and spore-bearing terrestrial fern, traditionally used in folk medicine. In the present study, the aqueous, ethanol and methanol extracts of the rhizomes of Tectaria macrodonta C. Chr were evaluated for its potential antibacterial and antioxidant properties. The phytochemical analyses of different solvent extracts were done. Antibacterial activity was determined by the agar well diffusion method against some gram-positive and gram-negative bacteria. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the plant extracts against the test bacteria were determined using the broth dilution method. The antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, ferric reducing power assay (FRAP) and hydroxyl radical (OH) scavenging assay. Potential antibacterial and antioxidant activity were exhibited by the methanol and ethanol extracts of Tectaria macrodonta. The methanol extract was subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The results of the present study suggest the potential pharmaceutical importance of Tectaria macrodonta C. Chr. concerning its antibacterial...
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