Posted by admin on Aug 31, 2019 in |
This approach was launched to evaluate the application of ultrasound waves for the preparation of 99mTc-Octreotide radio conjugate sample. The 740MBq (20 mCi) of 99mTc-Octreotide samples were prepared by the boiling water bath (standard) or ultrasound irradiation methods. The stability of samples in normal saline and human serum albumin was investigated. The partition coefficient and protein bonding of samples were determined. The radioisotope analysis was undertaken to assess the biodistribution of radiotracer samples in rats. The data obtained from this approach revealed that the 99mTc-Octreotide samples were successfully prepared by the newly developed technique. Statistically significant differences have not been observed in the stability, partition coefficient and protein bonding of radiotracer samples which were prepared by either of the two above-mentioned. The radioisotope analysis showed that the applied method for radiolabeling did not lead to a significant difference in the biodistribution pattern of radiotracer samples in rats. Green chemistry can be recommended for the radiolabeling of Octreotide with 99mTc as an alternative and reliable modality for the boiling water...
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Posted by admin on Aug 31, 2019 in |
Guanidines were prepared by the condensation reaction of primary amine (-NH2) with urea derivatives (R2NCONR2) in the presence of POCl3. In this research guanidine-pyridine, hybrid derivatives were synthesized by substituted urea derivatives namely 1,1,3,3-tetramethylurea and N, N′-dimethylurea with 2-aminopyridine. The metal complexes were obtained by reaction of metal(II) chloride with guanidine ligands in the molar ratio 1:1 and 1:2 (M:L). The structure of synthesized compounds was assessed by nuclear magnetic resonance (1H-NMR), Fourier-transform infrared spectroscopy (FT-IR), ultraviolet-visible (UV-Visible) mass spectroscopy studies and molar conductance. The measured molar conductance values indicated that the complexes were non-electrolytic. An octahedral geometry is proposed for the cobalt complexes but, square-planar geometry is suggested for both copper and nickel complexes. The synthesized compounds were also subjected to antibacterial study. Evaluation of antibacterial activities indicated that the metal complexes more inhibited the Gram-positive and Gram-negative bacteria strains as compared to the parent ligands. Among all the metal complexes the CuL2′ showed highest antibacterial activity with zone inhibition diameter of 29 mm and MIC value of...
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Posted by admin on Aug 31, 2019 in |
Neem (Azadirachta indica) is a member of the Meliaceae family and its role as the health-promoting effect is attributed because it is a rich source of potent biochemicals. It has been widely used in Chinese, Ayurvedic, and Unani medicines worldwide especially in Indian Subcontinent in the treatment and prevention of various diseases. In this present study’s author being investigated and identified biochemically active components in ether and methanolic extracts of stem bark of Azadirachta indica collected from the nursery of Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, India. The stem of the plant washed with water and methanol; dried plant material was successively extracted with petroleum ether at 34 ºC by Soxhlet apparatus for 72 h. The chemical formulas, double band equivalence and percentage of occurrence of the compounds have been elucidated with Fourier Transform Mass spectrometry with ESI detector in the negative mode as well as positive mode. The Instrument was run in full mode m/z (50-1000). METLIN database was used to identify the compounds. Many compounds were identified...
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Posted by admin on Aug 31, 2019 in |
Nanotechnology has brought about a significant change in the drug delivery system. A plethora of BCS class II drugs are formulated as solid lipid nanoparticles (SLN) due to its poor solubility and bioavailability and has potential applications in drug delivery system. The formulation of SLN involves use of a different type of surfactants and lipids in different concentrations which has shown a greater impact on various physicochemical parameters such as entrapment efficiency, drug release, particle size, zeta potential, storage and stability of the drug. Apart from its unique size-dependent property, merits and demerits, different excipients characterization technique, scale up, storage and stability are...
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Posted by admin on Aug 31, 2019 in |
This study aims to evaluate the in-vitro and in-vivo antioxidant activity of different extracts prepared from the aerial parts of Arthrophytum scoparium. Polyphenols flavonoids and tannins contents were determined. The acute toxicity of ASE was carried out based on OECD during two weeks to ascertain the LD50. In sub-acute toxicity, a 100, 200 and 400 mg/kg/day extract was orally administrated for 21 days consecutively. The results showed that decoction extract (DEC) was the richest fractions in Polyphenols, flavonoids and tannins content (23.29 mg/GAE, 99.77 mg/QE and 113.33 mg/TAE), respectively. Arthrophytum scoparium hydro-methanolic extract (ASE) had the strongest antioxidant activity using DPPH and ABTS scavenging assays with an IC50 of 0.045 and 0.0017 mg/ml, respectively. Moreover, the β-carotene/linoleic acid bleaching assay showed that ethyl acetate extract (EAE) had the highest inhibiting activity (83.29%). Furthermore, all A. scoparium fractions exhibited a good ferric reducing power. The administrated doses did not undergo changes in general behavior, toxicity or mortality of the tested mice. The LD50 was found to be superior to 5...
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