Posted by admin on Aug 31, 2024 in |
The objective of this research was to design and validate an alternative accurate method for quantitative determination of Amphotericin B. Amphotericin B deoxycholate life-saving drug belongs to the polyene class approved for the treatment of progressive and potentially fatal fungal infections. Amphotericin B was estimated at 382 nm and the percentage recovery was found to be 100.2 ± 0.34. The method was tested and validated for various parameters according to the ICH guidelines. The repeatability (inter-day) and intermediate precision (intra-day) precision studies revealed that the method is precise and reliable where all the RSD values were less than 2% for the routine analysis of drug. The limit of detection and limit of quantitation were 0.5μg/ml and 1.5μg/ml respectively. Results shows that the developed method is simple, reproducible and successfully for the estimation of Amphotericin B without the interference of common...
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Posted by admin on Aug 31, 2023 in |
Thrombosis is one of the major causes of death and disability worldwide. Cancer patients are commonly thrombophilia or prothrombotic since they have abnormalities in all three factors of Virchow’s triad, which lead to thromboembolism. Venous thrombosis (VT) is a chronic disorder that has the potential to be fatal and cause considerable complications. Venous thromboembolism (VTE) may potentially be the first indication of an undetected cancer in individuals. The rate of VTE and the risk of complications are much higher in the cancer patient groups. Arterial thrombosis is more likely to form when the plaque on the wall of the arteries consists a significant quantity of lipids covered by a layer of connective tissue. Thrombus formation in arteries may result in a heart attack or stroke. The hemostasis phase (liquid blood quickly hardens and forms a clot following injury to the blood vessel wall) spirals out of control. As a result, many blood clots form in the blood vessels that provide blood to various organs. Patients display paradoxically both excessive...
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Posted by admin on Aug 31, 2023 in |
Scenedesmus dimorphus isone of the widely exploited microalgae in several arenas of science, such as in food, nutraceuticals, agriculture, and pharmaceutical industries due to its high nutritional content and bioactivities. The present study designed a novel, cost-effective, eco-friendly, easy-to-operate and rectangular photobioreactor using glass material. Photobioreactor was provided with monochromatic LED light of 3000, 5000, 10,000 and 15,000 lux, agitation, and aeration facility. The effect of inoculum concentration, pH, temperature, and light intensity on the growth of S. dimorphus was evaluated in a novel photobioreactor. Finally, to convert dairy waste into valuable bioresources, dairy waste’s effect on microalgae growth was investigated in an optimized condition using a novel photobioreactor. The optimum inoculum concentration to obtain the highest growth of S. dimorphus was 1-2×107 cells/ml in proteose peptone medium (pH 6.8) at 20ºC with photoperiodicity of Dark/Light (D/L) 12/12h and 12,000 lux.In similar experimental conditions, S. dimorphus revealed maximum growth at pH 7.0 and the optimum light intensity was 15,000lux.Growth temperature optimization in optimum conditions with D/L 12/12h indicated significant...
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Posted by admin on Aug 31, 2023 in |
Parkinson’s disease (PD) is the second most prevalent type of neurodegenerative disorder and is a chronic, progressive condition. In the current investigation, we used a rotenone (ROT)-developing rat model of PD to assess the neuroprotective efficacy of ethanolic Withania somnifera root extract (EWSR) in comparison to the known neuroprotectants i.e., Doxycycline (Doxy), and ellagic acid (EA) individually and in combination (EWSR-Doxy-EA). Male Wistar rats were given ROT at a dose of 2.5 mg/kg body weight daily for 4 weeks to develop a PD in-vivo model. Once the PD model is confirmed, the rats were treated with individual neuroprotectants for next 5 weeks and kept under observation for next 7 days for reversal of PD. ROT administration has altered behavioural parameters (Cognitive, motor dysfunction, sniffing, grooming, raring, crossing, etc.) and neurotransmitter profile (Acetylcholine, epinephrine, nor-epinephrine, dopamine, glutamate and aspartate) in rats. The administration of neuroprotectants has ameliorated the deleterious effects of rotenone and restored cognitive and motor control by rectifying the levels of neurotransmitters which are responsible for the functions....
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Posted by admin on Aug 31, 2023 in |
Oral route is the easiest and most convenient route for drug administration more than 40% of new chemical entities exhibit poor aqueous solubility, resulting in unsatisfactory oral drug delivery. Self-nano-emulsifying drug-delivery systems (SNEDDSs) are one of the emerging strategies developed to tackle the issues associated with their oral delivery. SNEDDSs are composed of an oil phase, surfactant, and cosurfactant or cosolvent. SNEDDSs characteristics, their ability to dissolve a drug, and in-vivo considerations are determinant factors in the choice of SNEDDSs excipients. SNEDDS is a proven method for enhancing solubility and bioavailability of lipophilic compounds. Considering the ease of large-scale production and the robustness of SNEDDS, several formulations techniques are commercially available. The review provides exhaustive insight about the potential of SNEDDS in improving bioavailability of poorly soluble...
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