Posted by admin on Nov 30, 2025 in |
Mesalamine is a key therapeutic agent used in the treatment of inflammatory bowel diseases such as ulcerative colitis and Crohn’s disease. Ensuring its purity and controlling related impurities is critical for drug safety and efficacy. This study focuses on the development and validation of a robust reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous detection and quantification of three structurally related impurities Impurity G, Impurity K, and Impurity L in mesalamine pharmaceutical formulations. The chromatographic separation was achieved using an Agilent C18 column with an isocratic mobile phase composed of methanol and 0.1% acetic acid (52:48, v/v), at a flow rate of 0.85 mL/min, with detection at 229 nm. The method was validated in accordance with ICH Q2(R2) guidelines, covering critical parameters such as specificity, linearity (R² ≥ 0.999), precision (%RSD ≤ 0.65), accuracy (% recovery 94.8%–96%), robustness, limit of detection (0.20 µg/mL), and limit of quantification (0.62 µg/mL). The assay demonstrated high reliability for quality control applications and routine analysis of mesalamine products. Additionally, the method showed...
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Posted by admin on Nov 30, 2025 in |
The present study aimed to develop and validate a simple, precise, and accurate UV spectrophotometric method for the estimation of atorvastatin in bulk and pharmaceutical dosage forms. The method exhibited excellent linearity over the concentration range of 20–120 µg/ml with a correlation coefficient (R²) of 0.9996 and a regression equation of y = 0.01x + 0.0048. Precision was confirmed through intra-day and inter-day studies, showing low %RSD values of 0.2598 and 0.2987, respectively. Accuracy, evaluated via recovery studies at 80%, 100%, and 120% concentration levels, yielded a mean recovery of 99.65% ± 1.25, demonstrating the method’s reliability. The method also proved sensitive with a Limit of Detection (LOD) of 0.19872 µg/ml and a Limit of Quantification (LOQ) of 0.652387 µg/ml. Specificity studies confirmed no interference from formulation excipients. Additional parameters, including molar absorptivity (2289.23) and Sandell’s sensitivity (0.125), further validated the method’s robustness. The results affirm that this UV spectrophotometric method meets ICH validation criteria and is suitable for routine quality control of...
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Posted by admin on Nov 30, 2025 in |
Background: Psychotropic medications are integral to managing psychiatric disorders; however, their associated adverse drug reactions (ADRs) can significantly impair patients’ quality of life (QOL). Objective: To evaluate the spectrum of ADRs linked to psychotropic drug use and assess their impact on QOL among psychiatric patients at a tertiary care centre. Methods: In this cross-sectional observational study, 105 patients from the psychiatry outpatient and inpatient departments of JLN Medical College, Ajmer, were assessed. ADRs were documented using standard Pharmacovigilance Programme of India (PvPI) reporting formats. The WHOQOL-BREF questionnaire evaluated QOL. Causality and severity of ADRs were determined using the WHO-UMC scale and Hartwig’s criteria, respectively. Results: A total of 264 ADRs were recorded, with slurred speech (35.2%), rigidity (18.1%), and tremors (17.1%) being the most frequent. Antipsychotics, particularly olanzapine (22.8%) and haloperidol (19%), were commonly implicated. Most ADRs were non-serious (91.4%) and classified as probable (54%) or possible (42%). Patients experiencing moderate-to-severe ADRs showed significant declines in physical (p=0.004) and psychological (p=0.008) QOL domains. Conclusion: ADRs from psychotropic medications adversely...
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Posted by admin on Nov 30, 2025 in |
Background: Thyroid dysfunction is a common endocrine disorder affecting women’s reproductive health, manifesting as menstrual irregularities and infertility. Regional data from Northwestern Rajasthan remain limited. Objective: To evaluate the association between thyroid dysfunction and menstrual disturbances and fertility outcomes in women attending a tertiary care hospital in Northwestern Rajasthan. Methods: A hospital-based cross-sectional study was conducted over 12 months, enrolling 300 women aged 18–45 years presenting with menstrual irregularities or infertility. Clinical examination, menstrual history, and thyroid function tests (TSH, FT3, FT4) were evaluated. Women were classified as euthyroid, hypothyroid, or hyperthyroid based on standard reference values. Statistical analysis was performed using SPSS v25.0. Results: Thyroid dysfunction was found in 60.3% of participants (hypothyroidism 48.6%, hyperthyroidism 11.7%). Menstrual disturbances, including oligomenorrhea (37.9%) and menorrhagia (25.2%), were significantly higher in the hypothyroid group (p<0.001). Infertility was more prevalent in thyroid-dysfunctional women (42.5%) than euthyroid women (17.8%, p<0.001). Subclinical hypothyroidism was the most common abnormality (28.7%). Conclusion: Thyroid dysfunction, especially hypothyroidism, significantly affects menstrual and fertility outcomes. Early detection and management...
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Posted by admin on Nov 30, 2025 in |
Heterocyclic compounds have emerged as a focal point in medicinal and biological research due to their diverse applications. Their unique structures, often incorporating atoms like nitrogen, oxygen, or sulphur within their rings, offer a wide range of potential therapeutic properties. These compounds form the backbone of over 90% of novel drugs, bridging the gap between chemistry and biology. Their versatility extends to pharmaceuticals, agrochemicals, and veterinary products. Heterocyclic compounds exhibit a broad spectrum of biological activities, including antifungal, anti-inflammatory, antibacterial, antiviral, antioxidant, anticonvulsant, anthelmintic, antipyretic, anti-allergic, anti-histamine, herbicidal, anticancer, antihypertensive, and anti-leprosy effects. Beyond the existing market, numerous heterocyclic compounds are currently under investigation for their promising potential against various malignancies. Oxygen based heterocycles (Oxazole) serve as the foundational structures of numerous biologically active compounds and FDA-approved drugs. While nitrogen-based heterocycles have been extensively explored as potential anticancer agents, there is growing interest in oxygen and nitrogen-based heterocycles as a promising avenue for innovative drug discovery. Fusing multiple heterocyclic rings together is a common strategy to enhance the biological...
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