Posted by admin on Jun 1, 2013 in |
The present study was undertaken to investigate the hepatotoxic potential of rosiglitazone- an oral hypoglycemic thiazolidenedione class of drug. The hepatotoxic potential of rosiglitazone was assessed in-vitro on isolated rat hepatocytes before subjecting to in-vivo studies. The in-vitro activity was measured by trypan blue exclusion assay and determination of liver marker enzymes. The hepatotoxic potential of rosiglitazone was measured at 100 to 500 µg/ml concentration and maximum hepatotoxicity was produced at highest concentration tested. In-vivo hepatotoxic potential of rosiglitazone was assessed on hyperglycemic rats and protective effect of silymarin a known hepatoprotective agent was also tested. When rosiglitazone was administered at the dose of 2 mg/kg body weight for 21 successive days on rats, has produced marked hepatotoxicity when compared to normal animals. The hepatotoxic potential was evaluated by determination of serum biochemical markers and liver anti-oxidant enzyme studies. Hypoglycemic effect of rosiglitazone in presence of silymarin was also assessed in alloxan induced hyperglycemic rats to rule out any drug interaction. The rosiglitazone has produced significant elevation of serum...
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Posted by admin on Jun 1, 2013 in |
Solid lipid nanoparticle proved appropriate as prolonged release formulation for lipophilic drugs. The present work dealt with the preparation of the antifungal lipophilic drug nystatin into solid lipid nanoparticles ((Nyst-SLNs).) formulation for topical delivery with the aim to prolong its release and effectiveness. Hot homogenization and ultrasonication were applied in the production of (Nyst-SLNs). Different matrix material and stabilizers were tested. All the formulations were subjected to particle size analysis, zeta potential, drug entrapment efficiency and in-vitro release studies. Transmission electron microscopy was conducted to investigate the morphology of nystatin loaded nanoparticles. The differential scanning calorimetry visualized the dispersed amorphous state of nystatin in the SLN. In addition, the present study addresses the compositions of different formulation on the physicochemical properties and drug release profile of...
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Posted by admin on Jun 1, 2013 in |
In the present study, analgesic and anti-inflammatory activities of the synthesized agents derived from Ugi four component reactions (Ugi-4CR) has been described. The synthesized novel Ugi derivatives 7 (a-h) were characterized on the basis of I.R, 1H NMR and mass spectral analysis. Healthy Wistar Albino rats were used to carry out these activities. Analgesic activity was evaluated by Eddy hot plate method using Morphine Sulfate as a standard reference drug. Anti-inflammatory activity was evaluated by mercury displacement method using Diclofenac as standard reference drug. The results were expressed as mean ± S.E.M. The data were statistically analyzed by Student’s t test and P<0.05 were considered as significant. The screening data shows that synthesized compounds possess potential analgesic and anti-inflammatory...
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Posted by admin on Jun 1, 2013 in |
The purpose of this research was to develop and evaluate naturally obtained polysaccharides (Tamarind seed polysaccharide and Pectin) as a carrier in colon targeted drug delivery systems a colon targeted drug delivery. The newer developments in this context aim for an increased selectivity of drug delivery by targeting mechanisms which have a closer relation to pathophysiological particularities of the disease. The polysaccharides were characterized of its physical properties. The interaction between the excipcents and prednisolone was also studied through FTIR spectroscopy. Tablets were then prepared by wet granulation method with different ratio of polysaccharides and evaluated for their physical properties like weight variation, hardness, friability and content uniformity. In vitro drug release studies were performed in conditions simulating stomach to colon transit. No significant release was observed at acidic pH, however, when it reached the colonic pH where, drug release was observed. Also, release of drug was found to be higher in presence of rat caecal content. Data were fitted to various kinetic models. The mechanism of drug release...
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Posted by admin on Jun 1, 2013 in |
The use of traditional medicines of natural origin is being encouraged for the treatment of chronic disorders, as synthetic drugs in such cases may cause unpredictable adverse effects. The various strategies which have been identified to defeat drug resistance, the investigation of new and effective natural products exhibiting antimicrobial activity against pathogenic microorganisms is likely to play a significant role to overcome drug resistance. The numbers of global infections produced by bacterial strains that are resistant to single and multiple antimicrobial drugs are on the rise. With emerging trends in nanotechnology it has become possible to address the problems associated with potential natural products to be developed as antimicrobial drug. Nanomaterials can improve the pharmacokinetics, bioavailability, therapeutic index and specificity of plant origin drugs. By smartly designing nanoscale carriers, therapeutic value of natural products can be drastically improved, number of plant origin drugs can enter into clinical trials and antimicrobial resistance can be cured. Nanosizing led to increase solubility of components, reduction in the dose via improved absorption of...
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