Midazolam is used as short-acting pre-anesthetic benzodiazepine with solubility of <0.1mg/mL at neutral-pH, which increases considerably in acidic media. Midazolam commercial acidic aqueous parenteral formulation (pH~3.3) causes pain and inflammation at administration site, which induces low patient-compliance. According to the absence of non-parenteral formulation, available parenteral dosage-...
THE RELATIONSHIP BETWEEN INTESTINAL PROTECTIVE MARKERS AND SERUM ENDOTHELIN-1 CONCENTRATIONS IN LOW BIRTH WEIGHT INFANTS WITH HYPOXIC-ISCHEMIC ENCEPHALOPATHY
Perinatal hypoxia results in poor circulation in internal organs, causing damage to the intestinal mucosa. Here, we aimed to evaluate the role of endothelial vasoconstrictor function in the formation of the intestinal mucous barrier in low-birth-weight neonates with hypoxic-ischemic encephalopathy (HIE). We comparatively analysed the concentrations of specific intestinal protective markers, i.e., ...
CHARACTERIZATION AND VALIDATION OF IMPURITIES RELATED TO PHARMACEUTICAL BULK DRUG (API) BY USING SOME ANALYTICAL TECHNIQUES
Active Pharmaceutical Ingredient (API) of pharmaceutical bulk drug, four impurities were identified and were detected by a newly developed Reverse phase high performance liquid chromatographic (HPLC) method. The R.S.D.’s for SM-I, SM-II, SM- III and SM were found to be 1.48%, 1.71%, 1.67%, 6.37% respectively. These values are within the acceptance criteria of 10%.The limit of quantification valu...
VIRTUAL SCREEENING AND PHARMACOLOGICAL EVALUATION OF NEWER BTK INHIBITIORS AS POTENT ANTI-RHEUMATOID ARTHRITIC AGENTS
Rheumatoid arthritis is a chronic autoimmune disease affecting 1% of the world wide population. Recent researches suggested that the need for discovery of new therapeutic disease modifying anti-rheumatoid arthritis drugs (DMARD’s). BTK (Bruton’s tyrosine kinase) is cytoplasmic, non receptor tyrosine kinase and it is efficient target for the treatment of rheumatoid arthritis. This encouraged us...
Stress plays a major role in various pathophysiological processes related with neurodegenerative diseases and mental disorders. Zebrafish has emerged as a promising model organism in inducing Chronic Unpredictable Stress (CUS). In the present study, we have attempted to understand the molecular basis of neuronal changes underlying CUS in zebrafish model. An evidence for a connection between CUS an...
A PROSPECTIVE COMPARATIVE STUDY OF SAFETY OF LENALIDOMIDE PLUS DEXAMETHASONE COMBINATION THERAPY VERSUS VAD (VINCRISTINE, DOXORUBICIN AND DEXAMETHASONE) REGIMEN IN THE TREATMENT OF MULTIPLE MYELOMA
Background: Lenalidomide plus Dexamethasone (Len-Dex) and VAD (Vincristine, Doxorubicin and Dexamethasone) regimen are the two common drug therapies involved in the treatment of Multiple myeloma. These two groups of drugs act by different mechanisms and their safety profile also varies. Objectives: To compare the safety of Len-Dex versus VAD regimen based on World Health Organization toxicity crit...
IN VIVO ANTI-INFLAMMATORY ACTIVITY OF ETHYL ACETATE EXTRACT DERIVED FROM MARINE STREPTOMYCES CARPATICUS
Anti-inflammatory activity of the SC-EA extract of the marine Streptomyces carpaticus was studied in Wistar rats using acute and chronic inflammatory models like carrageenan induced paw edema and cotton pellet induced granuloma models respectively. The acute toxicity of SC-EA extract was tested on adult Wistar rats. The maximum dose of SC-EA extract at 2000mg/kg does not show any sign of visual ch...
Lantana camara Linn. is considered as a notorious weed and a popular ornamental plant. It is noted from ancient time that plants have been an excellent source of medicine. Since very long time Lantana camara has been reported as one of the most important medicinal plants in the world. Lantana camara is used in traditional medicine system for the treatment of cuts, swellings, ulcers, cataract, bili...
Vitex negundo, commonly called Nirgundi, is one of the widely investigated medicinal plants of India. The present study were planned to compare different parts, i.e. leaves, stems and roots of V. negundo for antianxiety activity using elevated plus maze model. Petroleum ether (60 - 80 °C), chloroform, ethanol and aqueous extracts of these parts were prepared and evaluated for anti-anxiety activit...
BISPHENOL A ENHANCES GROWTH OF Hep-G2 CANCER CELLS BY UPREGULATING EXPRESSION OF PRO – INFLAMMATORY AND PRO – ANGIOGENESIS PROTEINS
Bisphenol A (BPA) is primarily used in the manufacture of plastics and resins. There are many scientific reports that point to the adverse effects of this chemical to different animal models. There has been an increase in use of plastic during the last couple of decades and this has increased human exposure to plasticizers like Bisphenol A. But since little is known on how these endocrine disrupto...