HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC DETERMINATION OF COLCHICINE IN PHARMACEUTICAL PREPARATIONS AND BIOLOGICAL FLUIDS
A simple and rapid high performance thin layer chromatographic (HPTLC) method was developed for the assay of colchicine in bulk drug, pharmaceutical preparations and biological fluids. The method has a linear range of 4 - 35 μg/ml. The limits of detection and quantification were 1 and 4 μg/ml respectively. The results obtained by the proposed method were compared statistically by means...
Background: Malaria is a serious parasitic health problem in India. Plasmodium vivax and falciparum pose the greatest public challenge in endemic areas. P. vivax accounts for more than half of all malaria cases. Accurate and prompt diagnosis is not only important to prevent morbidity, but also helps minimizing drug resistance. Aim: To know the profile of malaria parasite in the patients attending ...
EVALUATION OF IMMUNOMODULATORY ACTIVITY OF POLYSACCHRIDE FRACTION OF INULA RACEMOSA, BOMBAX CEIBA AND ALLIUM SATIVUM
Inula racemosa, Bombax ceiba and Allium sativum are important class of drug known as “Rasayana”. The Polysaccharide fraction of all the three Drugs was isolated from hot water extraction yielding about 50-60% of fructan fraction (Polysaccharide). The polysaccharide fraction was evaluated for immunomodulatory activity by antibody titer and cellular immune response. The immunosuppressant drug az...
THE EFFECT OF PURIFIED NIGELLA SATIVA LECTIN AGAINST TUMOR DEVELOPMENT IN FEMALE ALBINO MICE AND GENETIC POLYMORPHISM IN PUMA GENE
The purpose of this study examine whether the polymorphism in p53 upregulated modulator of apoptosis (PUMA) gene consequential treated with purified Nigella sativa lectin in tumor induced mice. Nigella sativa (N. sativa) is a widely used medicinal plant throughout the world. Possess a seed lectin that was purified by combination of ammonium sulfate precipitation and affinity chromatography on a Se...
Arterial thrombus formation are due to mainly platelet activation and aggregation which are considered to be central to pathological changes occurring in the vessel leading to the various complications and mortality . Antiplatelet drugs are used to prevent and help in the reversal of platelet aggregation in arterial thrombosis which was the major culprit in the pathology of myocardial infarcti...
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF MARAVIROC IN BULK AND PHARMACEUTICAL FORMULATION BY UV SPECTROSCOPY
The aim of present work is to develop and validate simple, sensitive, economical and accurate Spectrophotometric methodhas been developed for determination of Maraviroc in pure form and in pharmaceutical formulations. Maraviroc in Phosphate buffer pH 7.4 shows maximum absorbance at 210 nm. Beer’s law was obeyed in the concentration range of 5-25 µg/mL, The LOD and LOQ were found to ...
The in vitro antioxidant activity of n-hexane, chloroform, ethyl acetate and alcoholic fraction of the total ethanolic extract of the leaves of Garcinia brasiliensis have been tested using various antioxidant model systems viz., ferric reducing power assay and hydrogen peroxide scavenging assay. Ethyl acetate fraction is found to possess higher ferric reducing power and superoxide radical scavengi...
EFFECT OF VIMLIV ON LIPID PEROXIDES AND ANTIOXIDANTS IN ETHANOL INDUCED HEPATOTOXICITY IN ALBINO WISTER RATS
This study was carried out to investigate the hepatoprotective and antioxidant properties of vimliv in ethanol-induced hepatotoxicity in rats. The liver toxicity was induced by the administration of ethanol to the animals at dose of 3 g/kg orally for 35 days. During the period of vimliv was co-administered to the rats at doses of 25 and 50 mg/kg for 35 days. The levels of lipidperoxidative product...
FORMULATION DEVELOPMENT AND IN-VITRO EVALUATION OF BUCOADHESIVE TABLET OF CHLORPHENIRAMINE MALEATE AND PHENYLEPHRINE HYDROCHLORIDE IN COMBINED DOSAGE FORM
Delivery of the desired drug as bioadhesive drug delivery systems has been subject of interest since 1980s. The various advantages associated with these systems made the buccal drug delivery as a novel route of drug administration. Buccal region offers an attractive route for the administration of systemic drug delivery. The objective of the study was to develop bucoadhesive buccal tablets of Chlo...
EFFICACY OF MASSOIA OIL IN COMBINATION WITH SOME INDONESIAN MEDICINAL PLANTS OILS AS ANTI-BIOFILM AGENT TOWARDS CANDIDA ALBICANS
Microbial resistance to antibiotics is often caused by biofilm formation of the microbial pathogen. One strategy used to combat this recalcitrance mechanism is by using the combination of antimicrobial drugs. Essential oils have evoked interest as sources of natural products and represent an alternative approach in combating microbial pathogen. The aim of the present work was to investigate the po...