Posted by admin on Feb 28, 2019 in |
Proteins play a significant role in living beings. In animals, where proteins are involved in the body built, the formation of tissues, organs, etc., in plants, proteins regulate the respiration, photosynthesis, growth, and reproduction. There are some proteins which are specific to the plant or a particular species of plant. Proteomics is a multidisciplinary branch of science that deals with the study of proteins. The paper discusses some important aspect related to estimation and identification of proteins. Protein was estimated in two plants of forensic importance, i.e., Ricinus communis and Cannabis sativa seeds by Ultra Violet (UV) spectrophotometry followed by isolation and characterization of proteins by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE) method. A UV spectrum was taken at 520 nanometers (nm), and the protein was quantified by the Folin-Ciocalteu method. SDS-PAGE was performed according to Laemmli et al. High concentration of protein was found by UV spectrophotometry at 520 nm. SDS-PAGE separated the proteins according to their molecular weight ranging in size from 45 kiloDalton (kDa) to...
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Posted by admin on Feb 28, 2019 in |
Objective: This study aimed at ensuring the different compositions of Valli panchmoola using authentic herbs and establishing pharmacognostical, physicochemical, and phytochemical standards for the compositions. Methods: Compositions of Vallli panchmoola were prepared as per the formula and procedure mentioned in traditional texts and Ayurvedic Formulary of India. The prepared compositions were evaluated for pharmacognostical, physicochemical, and phytochemical parameters using guidelines of the World Health Organization and Pharmacopoeial Laboratory for Indian Medicines for quality control of herbal drugs. Results: Microscopic studies revealed the presence of collenchyma cells, rosette crystals, compound starch grains, parenchyma cells, cluster crystal, etc. Loss on drying 0.17 ± 0.01%, total ash 1.87 ± 0.02%, etc. were observed in the classical group. Whereas loss on drying 0.10 ± 0.04%, total ash 1.77 ± 0.02%, etc. were observed in group-1. Preliminary phytochemical screening confirms the presence of alkaloids, flavonoids, glycosides, fatty acids, terpenoids, sterols, tannins, proteins, and phenolic compounds. Conclusion: The various pharmacognostical, physicochemical, and phytochemical standards will help in quality control/quality assurance and maintaining batch to batch...
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Posted by admin on Feb 28, 2019 in |
The present research work was planned to formulate poloxamer 407 based hydrogel formulations of metronidazole and the evaluation of various parameters like swelling behavior, drug PH stability, in-vitro and in-vivo drug release, and in-vitro cytotoxic activity. Two different concentrations of metronidazole hydrogel formulations were prepared using poloxamer 407 and were assessed by a validated HPLC method for drug content, pH stability, and in-vivo drug release. Further, in-vitro anticancer activity was evaluated using sulphorhodamine B (SRB) assay in SCC29 cell lines. Both the formulations F1 and f2 showed better pH stability at pH 3.5, 5.5 and 6.8. The formulation F1 was able to absorb about 152% of its weight of water within 80 min, whereas F2 absorbed 167.4% of its weight of water and remains constant over 100 min. In-vitro and in-vivo drug release pattern showed half-life at 6 h, AUC0−t 692 and 684ng h/ml, Cmax 1059 and 1142 ng/ml for F1 and F2 respectively. Hydrogel formulation F1 showed improved percentage control growth when compared to F2 hydrogel formulation and...
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Posted by admin on Feb 28, 2019 in |
The present work encompasses the development of two classical UV spectroscopy methods and an RP-HPLC method for the concurrent assessment of Halobetasol propionate and Mupirocin inculcating a statistical approach. The UV spectroscopy methods developed herein include absorption ratio method and first derivative spectroscopy method. The wavelength maxima selected for Absorption ratio method were 240 and 220 nm for Halobetasol Propionate and Mupirocin respectively. The zero crossing points selected were 240.626 nm and 220.180 nm for Halobetasol Propionate and Mupirocin respectively for first derivative spectroscopy. The chromatographic separation was achieved by using CHROMBUDGET C18 (250 × 4.6 mm) 5 µm column, mobile phase consisting of Acetonitrile: phosphate buffer (65:35 v/v, pH 3.2), at 1 ml/min flow rate and detection wavelength was 230 nm The retention time of Halobetasol propionate, and Mupirocin was found to be 8.647 ± 0.06 min and 3.357±0.123 min, respectively. Linearity was observed in the concentration range of 5.125 – 5.75 µg/ml for Halobetasol propionate and 5-30 µg/ml for Mupirocin respectively. The analytical method was validated as...
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Posted by admin on Feb 28, 2019 in |
Aim: To determine the prevalence of metabolic syndrome in Udhampur, J & K population according to two different criteria’s (IDF & NCEP-ATP III). Methods: A cross-sectional survey was conducted for the prevalence of metabolic syndrome among 210 participants. A pre-designed questionnaire was used to collect data, including demographic information; physical activity and biochemical parameters form their medical reports. Results: The study participants with a mean age of 55.79 yr consisted of 51.9% males and 48.09% females. Metabolic syndrome was seen in 46.67% and 35.24% subjects according to IDF and NCEP-ATP III criteria, respectively. The age-specific prevalence of metabolic syndrome was observed higher in the older group. Conclusion: It can be improved by encouraging the patients to adopt a healthy lifestyle with the intervention of regular physical activity and dietary...
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