Volume 13 (2022) - Issue 3, March
Tissue specificity is the essential need of the hour to cut down clinical abnormalities. Allowing desired drug concentration to reach the target site is the current trend of the drug delivery system. Laminin is an active component of the basal lamina and present in most cells and organs has shown great utility in this case. It is a heterotrimeric glycoprotein containing an α-chain, a β-chain, an...
Arbuscular mycorrhizal fungi (AMF) enjoy being the cynosure in most agro-ecosystems. These fungi benefit the plant in multiple ways. They improve plant growth by enhancing nutrient uptake and protecting the plant from various plant pathogens, thereby promoting plant diversity. These fungi improve plant tolerance to various abiotic stresses, such as salinity, drought, extreme temperatures, and heav...
QUALITATIVE AND QUANTITATIVE ESTIMATION OF TYROSINE KINASE INHIBITORS IN PHARMACEUTICAL AND BIOLOGICAL MATRICES – AN OVERVIEW
Tyrosine kinase inhibitors generally having a narrow therapeutic window and large inter-patient variability. In order to support its therapeutic drug monitoring, fast and accurate methods are needed to supporting the pharmacokinetic-guided dosing in patients treating with various tyrosine kinase inhibitors (TKIs) either alone or in combination in bulk, formulations or human plasma using HPLC and d...
The bioavailability of conventional ophthalmic solutions is very poor due to efficient protective mechanisms of the eye, blinking, reflex Mental health problems are characterized by in definite persistently depressed mood, low self-esteem, loss of interest in normal life activities. A common problems were the chances to occurs from generation to generation, i.e., genetic in nature, and these may a...
The aim of this review is to provide an extensive overview of the diverse pharmacological activities of quinazoline moiety. Quinazolines are well-known and important nitrogen-containing heterocyclic compounds made chemical formula C8H6N2. For the last few years, the heterocyclic fused nucleus quinazoline has drawn immense attention due to its diversified application in medicinal chemistry research...
RATIONAL APPROACHES FOR SYNTHESIS OF SOME NOVEL IMIDAZOLE HETEROCYCLES WITH THEIR BROAD SPECTRUM OF PHARMACOLOGICAL ACTIVITIES: A BRIEF REVIEW
Imidazole has a special role in heterocyclic chemistry, and its derivatives have piqued interest in recent years due to its diverse chemistry and pharmacology properties. Imidazole is a nitrogen-containing heterocyclic ring that is essential in biology and pharmaceuticals. As a result, imidazole compounds have piqued the attention of researchers for more than a decade. Thus imidazole compounds hav...
In this review, we are completely discussing different eras in pharmaceutical drugs/medicines, i.e., Biologics and biosimilars. These biologics can be called life drugs because they are deriving from living organisms, microbes, yeast, bacteria, and human blood products. These biologics are biopharmaceutical products that are derived from living organisms and are very complex in molecular structure...
VALIDATION THROUGH RE-DOCKING, CROSS-DOCKING AND LIGAND ENRICHMENT IN VARIOUS WELL-RESOLUTED MAO-B RECEPTORS
Molecular docking is one of the most utilized in silico techniques, which drastically reduces the cost and the time needed for the design of novel drugs. Recently, the tremendous growth of resolved crystallographic MAO-B structures, together with the rapidly rising computing power, has resulted in accelerated and moderately correct docking studies. However, initial validations of the docking proto...
PREDICTION OF HOTSPOT IN PROTEIN-PROTEIN/PROTEIN-SUBSTRATE INTERACTION: A NOVEL COMPUTATIONAL APPROACH
Protein-protein, as well as protein-substrate interactions, play an important role in regulating specific biological functions like signal transduction, apoptosis, gene regulation, and immune response. These play a major role in drug discovery applications and can be used as molecular targets to stop disease development at the molecular level. Thus, understanding the biological mechanism of protei...
Valtrate is one of the major valepotriates reported from various species of the genus Valeriana. Aim of the present study was to develop and validate analytical method for quantification of valtrate in dried roots of Valeriana jatamansi and Valeriana pyrolaefolia, both members of family Valerianaceae. Acetonitrile and water (70:30) in isocratic mode were used with an aflow rate of 1.5 mL/min (at 2...
ANTIBACTERIAL ACTIVITY OF SOME COMMON KITCHEN SPICES AGAINST ESCHERICHIA COLI, STAPHYLOCOCCUS AUREUS, SALMONELLA TYPHIAND PSEUDOMONAS AERUGINOS
The current study investigated and compared the antibacterial activity of common kitchen spices; Ginger, Turmeric, Bay leaf, and Coriander, against Escherichia coli, Staphylococcus aureus, Salmonella Typhi, and Pseudomonas aeruginosa. The antibacterial activity of different spices was calculated by the agar well cup plate method. The concentrations, 75%, 50%, and 25%, were made for all the test sp...
STABILITY INDICATING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF ACEBROPHYLLINE AND DOXOFYLLINE IN PHARMACEUTICAL DOSAGE FORM
Objective: To develop a simple, selective, and precise stability-indicating high-performance liquid chromatography method for the simultaneous estimation of acebrophylline and doxofylline in bulk and tablet dosage form. Methods: The chromatographic separation achieved on HiQSil C18 Column (250 × 4.6 mm, 5µm) utilizing a mobile phase Acetonitrile: 10 mM n-hexane sulfonic acid buffer (80: 20, v/v)...
DEVELOPMENT AND EVALUATION OF POMALIDOMIDE SUPERSATURABLE SELF-NANOEMULSIFYING DRUG DELIVERY SYSTEM FOR IMPROVED SOLUBILITY AND DISSOLUTION RATE
Background: Pomalidomide belongs to BCS class IV drug with low solubility and undergoes first-pass metabolism leads to reduced bioavailability of 15%. Objective: The current study aimed to develop pomalidomide Super saturable-SNEDDS to enhance solubility. Methods: Preliminary solubility studies were performed to identify oil, surfactant, and co-surfactant ratios. Pseudo tertiary phase diagram cons...
THE EFFECT OF FRACTIONS OF INDIGOFERA ASPALATHOIDES LEAF EXTRACT ON EXTEND OF LIPID PEROXIDATION AND ANTIOXIDANT STATUS IN DMBA INDUCED HAMSTER BUCCAL POUCH CARCINOMA.
Oral cancer is the sixth leading cancer globally and the leading carcinoma in the Indian subcontinent. The relatively high incidence is due to usage of tobacco, and exposure to carcinogens are frontrunners of oxidative stress-induced DNA damage. The practice of chemopreventive agents is considered to be alternative oral carcinoma. A study on novel natural chemo preventive agent-Indigofera Aspalath...
Ocular deliveries possess a challenging pharmaceutical approach due to poor absorption index through the corneal appendages and washing away of the drug through tears and lachrymal secretion. The ocular appendages lead to provide lesser bioavailability of conventional dosage forms; hence, frequent dosing is advised to get the optimum effect of the medicine. Therefore, in-situ gel offers a promisin...
There is an urgent need to replace the injection currently used for low molecular weight heparin (LMWH) multi-dose therapy with a non-invasive delivery device. In this study, unsaturated polyester resin and peroxide were used to fabricate microneedle mould by using 3M microneedle. Dissolving microneedle arrays transdermal patch was prepared by using 20 % w/v polymers of PVA and PVP (polyvinyl alco...
IN-VITRO AND IN-VIVO EVALUATION OF ANTI-ASTHMATIC ACTIVITY OF CALOTROPIS PROCERA ROOT EXTRACTS OF CALOTROPIS PROCERA
Objective: The Calotropis procera (Ait.) R.Br. (Ascelpidaeaceae) has been used traditionally as anthelmintic, depurative, diaphoretic, emetic, expectorant, febrifuge, and purgative, but no scientific reports are available in the regulation of asthmatic condition. In this present study, Calotropis procera (Ait.) R.Br. was selected to investigate scientifically to establish scientific data for its...
ANTIMICROBIAL AND ANTIFUNGAL ACTIVITY OF BARK OF HARDWICKIA BINATA ROXB (FABACEAE / CAESALPINIACEAE)
Background and Objectives: This study was carried out with the objective to investigate the antibacterial and antifungal activity of the bark of Hardwickia binata Roxb. Materials and Methods: Methanolic extract of bark of Hardwickia binata Roxb. exhibited an inhibitory effect towards the pathogenic organisms. Antimicrobial activity the zone of inhibition values of methanolic extract of bark of Har...
The objective of the present study was to develop cyclodextrin-based nanosponge based topical gel of tazoretene using cross-linker diphenylcarbonate and Carbopol® Ultrez 10 NF polymer. The β-Cyclodextrin nanosponges were prepared using various concentrations of β-Cyclodextrin and diphenyl carbonate and characterized. Based on evaluation parameters the β-Cyclodextrin nanosponges formulations (N...
The work aimed to prepare super porous hydrogels (SPHs) of Amoxicillin trihydrate to release the drug in a sustained manner in the gastric environment and study the polymeric ratio and pore effect formers on the physicomechanical properties and drug release kinetics of the formulations. Fourier Transform Infra-Red (FTIR) studies were done to check the compatibility of the drug with the polymers. T...
ISOLATION, CHARACTERIZATION AND EVALUATION OF ANTI-ULCER ACTIVITY OF PHYTOCONSTITUENTS PRESENT IN MORINGA OLEIFERA LEAF
Moringa oleifera leaf is widely used in the traditional medicine system and also used as a food by different communities and local tribes of Assam in India. Moringa oleifera leaf contains various bioactive compounds such as alkaloids, flavonoids, rutin, steroids, etc. The flavonoids quercetin act as an anti-ulcer agent, and rutin have a gastro cytoprotective effect i.e., present in M. oleifera lea...
DEVELOPMENT AND VALIDATION OF A SENSITIVE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) METHOD FOR THE ESTIMATION OF CURCUMIN IN RAT PLASMA
Curcumin is used as a potent anti-inflammatory agent in the treatment of rheumatoid arthritis and its clinical pharmacokinetics requires a sensitive method for estimation of its plasma concentration. Hence the aim of the study was to develop a rapid, selective, and sensitive HPLC method coupled with UV detection for the determination of Curcumin in rat plasma. The process of elution was carried ou...
Aceclofenac is a non-steroidal anti-inflammatory drug indicated for symptomatic treatment of pain and inflammation. Aceclofenac is a BCS Class II drug with low solubility and high permeability, thus solid dispersion is one of the promising techniques used for enhancement of solubility and dissolution rate. The intent of the present study was to formulate and evaluate aceclofenac matrix tablets by ...
The purpose of the present study was to optimize palbociclib-loaded solid lipid nanoparticles (SLNs) by evaluating the relationship between design factors and experimental data. A three-factor, three-level Box-Behnken design (BBD) was used for the optimization procedure, choosing the amount of tricapric, cremophor RH40 and soy lecithin, as independent variables. The chosen dependent variables were...
Background: Satranidazole is a class II drug per biopharmaceutical classification systems with poor aqueous solubility and antiprotozoal and anti-bacterial properties. Aim: Therefore, the purpose of the present study was to enhance the solubility of Satranidazole using a self-emulsified drug delivery system (SEDDS). Methods: SEDDS was prepared using Satranidazole (10 mg) and dissolved in the oil p...
ASSOCIATION OF LEVELS OF VITAMIN-D AND MANNOSE-BINDING LECTIN IN CASES OF DENGUE FEVER IN RURAL INDIA
Background: Dengue is an arthropod-borne viral disease caused by the dengue virus (DENV). DENV is an RNA virus that belongs to the Flavivirus genus. The incidence of dengue has grown in the recent decade globally. The World Health Organization (WHO) considers dengue as a major global public health challenge in the tropic and sub-tropic nations. Materials and Methods: NS1 antigen and IgM antibody E...
Standardization of herbal formulation is essential to assess the quality of drugs for its therapeutic efficacy. Pancha Haritha kadi Churna (PHC) is a traditional poly herbal formulation meant for digestion which consists of five ingredients having digestive property. It is used for the treatment of Constipation and Bloating. Churna’s will play an important role in gastro intestinal problems and ...
EARLY TREATMENT WITH PREDNISOLONE AND ACYCLOVIR IN SARS-COV-2 COMPLICATED BELL’S PALSY: A CASE REPORT
Bell's palsy is an idiopathic, unilateral, acute weakness of the face in a pattern consistent with peripheral facial nerve dysfunction and may be partial or complete, occurring with equal frequency on either side of the face. The incidence is about 20 in 100,000 people a year, with about 1 in 60-lifetime risks. Bell's palsy has a peak incidence between the ages of 15 and 40 years. Viral infections...
DEVELOPMENT OF SELF NANO EMULSIFYING DRUG DELIVERY SYSTEM OF DORAVIRINE: SOLUBILITY AND DISSOLUTION RATE IMPROVEMENT
Doravirine is an HIV-1 non-nucleoside reverse transcriptase inhibitor (NNRTI). The aim of the present investigation was to develop a self-nanoemulsifying drug delivery system (SNEDDS) to enhance the solubility and dissolution of poorly water-soluble doravirine. The solubility of doravirinein various oils was determined to identify the oil phase of SNEDDS. Various surfactants and co-surfactants wer...
Millets are called nutri-cereals, highly nutritious, and are known to have high nutrient content, which includes protein, essential fatty acids, dietary fiber, B-Vitamins, minerals such as calcium, iron, zinc, potassium, and magnesium. They help render health benefits like reducing blood sugar level (diabetes), blood pressure regulation, thyroid, cardiovascular and celiac diseases. The present stu...
DESIGN AND CHARACTERIZATION OF TASTE MASKED DEXTROMETHORPHAN HBR ORAL DISINTEGRATING TABLET BY USING NATURAL SUPERDISINTEGRANTS
Objective: Our work was to develop an ordispersible tablet of Dextromethorphan HBr with acceptable palatability to help patients of all age groups. Methods: The bitter taste of the drug was masked by entrapping the drug into Eudragit RL 100. The microspheres were prepared from solvent evaporation method. The effect of particle size, drug entrapment efficiency, % yield and % drug release of the mic...
The aim of the present work is to develop and optimize solid lipid nanoparticles (SLNs) of pomalidomide with the aid of Box-Behnken design (BBD). A design space with three formulation variables at three levels was evaluated in BBD. Amount of lipid (A), surfactant (B), and co-surfactant (C) were selected as independent variables, whereas particle size (Y1), entrapment efficiency (Y2), and percentag...
DEVIATION IN WHO CORE PRESCRIBING INDICATORS AMONG SURGICAL PATIENTS IN SECONDARY CARE HOSPITAL: THE NEED FOR RATIONAL PRESCRIBING
Irrational use of medicines is a major problem worldwide. In surgical patients, a greater number of drugs are prescribed So, evaluating prescribing patterns of various medications in surgical inpatients would enable identifying the presence of irrational use, thereby paving a way to rational prescribing. A prospective observational study was conducted in a secondary care hospital for six months. T...
The present study aims at analyzing the efficiency of allicin, agarlic derived phytochemical or neutraceutical in elevating the activities of various enzymic antioxidants on its treatment to cultured breast cancer cell lines. In the experimental work, cultured breast cancer cell lines were treated with different doses of allicin, and through standardized protocols, the cell lines were assayed for ...
ASSESSMENT OF METHODS USED BY REGISTERED PHARMACISTS FOR DISPOSING OF THE PHARMACEUTICAL WASTE AND EXPIRED MEDICINES AT COMMUNITY PHARMACIES IN URBAN AREA
Introduction: The use of improper disposal methods for expired medicine is an emerging issue that is affecting the ecosystem, and misuse of expired medicines is potentially hazardous for public safety. Our study was aimed to assess the methods used by the registered pharmacist for disposing of the expired medicines in community pharmacies. Materials and Methods: A questionnaire-based cross-section...
STUDIES ON SOLID DISPERSION OF CURCUMIN USING HYDROPHILIC POLYMER WITH SYLOID XDP AND LOZENGE CHARACTERIZATION
Curcumin, an active phytochemical compound of the rhizome Curcuma longa, shows several pharmacological activities. Curcumin shows high permeability and low dissolution rate, and its incomplete absorption leads to very poor oral bioavailability. The present study aimed to enhance curcumin's solubility and dissolution rate by forming internal ternary solid dispersion using hydrophilic polymer PVP K3...
A COMPARATIVE ANIMAL STUDY OF ANALGESIC EFFECTS OF TRANDOLAPRIL AND NIMODIPINE IN MICE USING EDDY’S HOT PLATE
Angiotensin II from the renin-angiotensin system is proposed to be pro-inflammatory induces reactive oxygen species, nuclear factor kappa B. It sensitizes pain receptors to mediators of pain (via PGE2). ACEI block these actions increase endogenous opioids by inhibiting enzyme enkephalinase. Voltage-gated calcium channels (VGCC) causing calcium influx are involved in exocytosis of synaptic vesicle ...
Introduction: Anabaena azollae (AM) is used as a biofertilizer feed for cattle; much research is developing toward identifying the elements and their role in food supplements for human consumption. Objective: The study was to characterize the whole plant powder of the A. microphylla concerning physicochemical and elemental analysis. Material and Methods: The whole plant powder was subjected to phy...
Polygonum glabrum, commonly called dense flower knotweed, is a perennial plant found in most parts of India. The leaves of plants Polygonum glabrum Willd. Belonging to the family, Polygonaceae are a rich source of flavonoids and tannins but not yet scientifically investigated for antioxidant activity. The present study aimed to offer a novel scientific background to the leaves of Polygonum glabrum...
EVALUATION AND ASSESSMENT OF ADVERSE DRUG REACTIONS DEVELOPED USING NEWLY PRESCRIBED DRUGS IN PATIENTS WITH ACUTE DISEASE
The objective of current research is to assess the type of Adverse Drug Reactions (ADR) and associated risk factors, assess the prevalence, estimate the incidence of serious and fatal ADR and the severity of ADRs based on data collected from patients with ADRs caused by drug initially prescribed. Data of patients with acute diseases were collected and analyzed using SAS version 9.1. About 252 ADRs...
UV-SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF HYDROXYCHLOROQUINE SULFATE AND NITAZOXANIDE IN SYNTHETIC MIXTURE
A simple, accurate, precise, reproducible, economical UV spectrophotometric method has been developed and validated for simultaneous estimation of Hydroxychloroquine sulfate (HCQ) and Nitazoxanide (NTZ) in a synthetic mixture. Method A by simultaneous equation method, Method B by the first-order derivative method. Method A: simultaneous equation method was based on the measurement of absorbance of...
This study looked at heavy metal levels in selected registered herbal drugs of Asian and unregistered Ghanaian herbal drugs sold in Kumasi. It examined the health risks of the metals. It provided authorities with data to ensure that herbal drugs are strictly monitored and their metal levels evaluated to safeguard patrons' health. Dry ashing method was used on 10g of each of the herbal drug samples...
The study was designed to study the macroscopic, microscopic, and also physicochemical parameters such as fluorescence analysis, extractive values, ash values, and loss on drying were performed. Therefore, this parameter is essentially used to control the quality of crude drugs and/or herbal drugs/drug products. The moisture content (% LOD) of the leaf powder was found to be 5.10± 0.14 (% w/w), t...
OBSERVATIONS ON CARDIAC ABNORMALITIES IN PATIENTS WITH DEXTROCARDIA AND SITUS AMBIGUUS: A RETROSPECTIVE STUDY
Studies on dextrocardia have been limited by low numbers. Hence, finding the most common diagnosis in patients with dextrocardia and situs ambiguus is challenging among patients seeking medical attention in a tertiary care center. Aims and objectives: To identify the most common diagnostic pattern in dextrocardia (DC) patients with situs ambiguous (SA). Methods: It is a retrospective study with re...