DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF PREDNISOLONE ACETATE AND OFLOXACIN IN EYE-DROP
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical spectrophotometric method for the simultaneous determination of prednisolone acetate and ofloxacin in combined eye drop dosage form. The method is based on the simultaneous equations method for analysis of both the drugs using methanol as solvent. Prednisolone acetate has absorbance maxima at 243 nm and ofl...
DETERMINATION OF PIOGLITAZONE HYDROCHLORIDE IN BULK AND PHARMACEUTICAL FORMULATIONS BY UV SPECTROPHOTOMETRIC METHOD
UV spectrophotometric method was developed and validated for the analysis of pioglitazone in tablets. The analyses were performed in Phosphate buffer (pH 7.4). Beer’s law was valid in concentration range of 10-50 mcg/ml and UV detection was done at 238 nm. The developed method was validated respect to linearity, precision, accuracy, selectivity / sensitivity applied to the determination of Piogl...
EVALUATION OF ANXIOLYTIC ACTIVITY OF HYDROALCOHOLIC ACTIVITY OF TEPHROSIA PURPURIA (L) PERS ON SWISS ALBINO MICE
The present work is to evaluate the anxiolytic activity of a hydroalcoholic extract of Tephrosia purpuria (L) Pers (HAETP) in mice using the elevated plus-maze (EPM), elevated zero-maze (EZM), Y-maze and hole-board models. Furthermore, the anxiolytic effects of HAETP were compared to a known active anxiolytic drug diazepam. The extract, administered orally, in two different doses of HAETP 200 mg/k...
Comparative and synergistic antioxidant properties of Carica papaya leaf (CPL) and Azadarichta indica leaf (AIL), which are popularly used as medicinal plants were evaluated by analyzing the individual leaves and their 1:1 (mass/mass) homogenous combination (CPL+AIL) concurrently. The antioxidant properties measured were the levels of total phenol, tannin, total flavonoid, total carotenoid, vitami...
Nephrotoxicity is one of the most common kidney problems and occurs when body is exposed to a drug or toxin. A number of therapeutic agents can adversely affect the kidney resulting in acute renal failure, chronic interstitial nephritis and nephritic syndrome because increasing number of potent therapeutic drugs like aminoglycoside antibiotics, chemotherapeutic agents and NSAIDS. Nephroprotective ...
Context: Nigella sativa Linn. (Ranunculaceae) is native to southwest Asia. This tree species has been of interest to researchers because it has a long history of folklore usage in various systems of medicines. Objective: Pharmacognostic Standardization, Physico and Phytochemical Evaluation of the seeds of N. sativa were carried out to determine its macro-and microscopical characters and also s...
Herbalism is a traditional medicine practice based on the utilization of plants and plant extracts. In herbalism ‘Valerian’ is a boon medicine for several ailments. Valerian (Valerian officinalis) is a naturally occurring Ayurvedic medicine widely grown in the temperate zones of Europe, North America and Asia and is a safe herbal supplement indicated in various ailments, chief being for the sy...
Recently much attention has been paid to the application of microemulsion as drug delivery system. Part of this interest appears as a consequence of their transparency, ease of preparation and long-term stability. These properties as well as their ability for incorporating drugs of different lipophilicity are some of the reasons why microemulsions have been thoroughly considered for pharmaceutical...
IN-VITRO ANTIMICROBIAL ACTIVITY OF AEROMONAS SPP ISOLATED FROM ESTUARY USING DIFFERENT SCREENING PROTOCOLS
Screening is important in investigating antimicrobial activities of bacteria in a quest to discover new antimicrobials. Various protocols are readily available and employed by researchers worldwide studying microbiostatic and microbicidal activities of bacterial. This research therefore aims at evaluating available protocols and suggesting a standardized model. For the purpose of these research, a...
Carissa spinarum L. is a small spinous evergreen shrub growing throughout India in dry regions.The plant used locally in Indian and Chinese system of medicines to cure various diseases. Carissa spinarum has also been used in the Ayurvedic medical system because of its therapeutic effects against liver disease, epileptic disease, microbial disease, cytotoxic, viral diseases.Th...