Volume 14 (2023) - Issue 5, May
WOUND HEALING PERSPECTIVES OF PROMISING HERBAL RESOURCES: A REVIEW
A wound is described as a break in a tissue's cellular and anatomic continuity. A wound is a cellular, anatomical, and functional disturbance of living tissue induced by physical, chemical, electrical, or microbiological threats to the tissue. Wound healing is a multi-step process involving injured tissue regeneration or restoration. The typical wound-healing response is a coordinated series of pr...
SAGA OF GASTRORETENTIVE DRUG DELIVERY SYSTEM: EMERGING CONCEPTS, RECENT ADVANCES AND TECHNOLOGICAL PROGRESS
Oral controlled release and site-specific drug delivery systems have gained interest in the pharmaceutical industry to improve the treatment of certain critical medications. To overcome physiological barriers, such as short stomach resident lengths and variable gastric emptying periods, the idea of a novel drug delivery mechanism evolved. To improve the bioavailability of drugs with narrow therape...
A REVIEW OF THE ANALYTICAL METHODS FOR THE DETERMINATION OF FAVIPIRAVIR
Favipiravir is, an antiviral agent, used to treat diseases caused by RNA viruses like Ebola virus, SARS-CoV-2, Influenza virus, Polio measles etc. The prodrug Favipiravir enters the infected cells through endocytosis and undergo metabolism to become an active drug. An active form of Favipiravir selectively targets the catalytic domain of RNA-dependent RNA polymerase and then interrupts the nucleot...
PHYTOCHEMICAL AND PHARMACOLOGICAL OVERVIEW OF WITHANIA COAUGULANS
Withania coagulans, commonly known as Rishyagandha in Sanskrit and Paneer phool in Hindi, is a plant in the Solanaceae or nightshade family, native to Afghanistan, Pakistan and the Indian subcontinent. Within the genus Withania, W. somnifera and W. coagulans are economically significant and are cultivated in several regions for their use in Ayurveda. Roots of W. somnifera have been kno...
A CRITICAL REVIEW ON THE MEDICINAL HERB AEGLE MARMELOS (LINN.) CORREA
Plants are essential to the survival of every species on Earth. Animal and plant life have a history of mutual consumption since antiquity. Earth's sole producers are plant is a fact. As researchers learn more about plants, this assumption is being challenged. The Rutaceae family's citrus species Aegle marmelos (Bael), a well-known medicinal herb, has a lengthy study history. In addition to its us...
A METHODICAL REVIEW ON OMICRON -A NEW VARIANT AS A DRUG DEVELOPER
In mid-November 2021, the new OMICRON variety was first discovered in South Africa. As of today, the OMICRON version already appeared on December 15, 2021. Around 77 countries are affected, with the bulk of cases originating in the United States, India, the United Kingdom, and South Africa. OMICRON-positive instances were also reported. The first mortality associated with the novel COVID-19 mutati...
AN INTERPRETATION OF CARDIOVASCULAR COMPLICATIONS IN COVID-19 PATIENTS AND POST-COVID-19-VACCINATION, A PERUSAL
COVID-19, caused by Severe Acute Respiratory Syndrome CoV-2 (SARS CoV-2), has become a global burden. The naïve era of infection prompted early dependence on case reports of insufficient data and conceptual elucidation to explain and anticipate the effect on cardiovascular diseases. Many COVID-19-infected and vaccinated individuals have reported an increased incidence of cardiovascular disorders,...
NANOSUSPENSION – A PROMISING TECHNIQUE FOR POORLY SOLUBLE DRUGS
Most pharmaceutical drugs create problems during formulation as they are insoluble or poorly soluble in aqueous and non-aqueous mediums. It was proved that reducing the drug particle size increases the drug's Solubility, Dissolution and Bioavailability. Many conventional techniques may be used, but the desired amount of particle size may not be achieved, and Solubility, Dissolution and Bioavailabi...
RECENT ADVANCEMENTS IN THE FORMULATION AND EVALUATION OF MICROPARTICLES AND ITS APPLICATION
Introduction: The micro particles and development in the area of micro particles analysis are discussed in this article. Areas Covered: Micro particles are a unique drug carrier method that provides an effective therapeutic alternative to single-unit dose forms that are either traditional or rapid release. Micro particles are produced by filling them with firm gelatin or compressing them immediate...
A CRITICAL REVIEW ON THE CONTRACEPTIVE AND ABORTIFACIENT MEDICINAL HERB’S DESCRIBED IN BHAVAPRAKASHA NIGHANTU
Bhavaprakasha is Ayurveda's important nighantu (Lexicon) written in the sixteenth century AD. Many useful herbs are mentioned in Bhavaprakasha Nighantu that can be used daily. However, one common misconception is that all ayurvedic drugs are safe and devoid of any side effects since they are of herbal origin is not true. Ayurvedic herbs sometimes become harmful based on the condition of the patien...
DIFFERENT TREATMENT APPROACHES USED IN AUTOIMMUNE THYROID DISEASE: A COMPREHENSIVE REVIEW
Autoimmune thyroid disease triggers because of T cells attack in the immune system (in the thyroid gland). AITD mainly comprises of two types- ‘Hashimoto’s thyroiditis’ (hypothyroidism) & ‘Grave’s Disease’ (thyrotoxicosis). India has ‘42 million’ people groups experiencing thyroid disease, likewise it has perceived that in contrast with men 80% women affect more from immune sys...
RECENT BREAKTHROUGHS IN THE MANUFACTURE OF MICROBIAL PROTEASES AND THEIR APPLICATIONS: A REVIEW
Enzymes are biocatalysts that accelerate biotic reactions positively or negatively. Proteases are small types of enzymes that cleave peptide bonds. These proteases can be produced by microbes (fungi, bacteria, and viruses), plants, and animals. The catalytic site is only visible from the outside and is broadly dispersed on the surfaces of different cell types. Based on the position of the cleavage...
AN OVERVIEW OF THE DEVELOPMENT OF INSULIN DRUG DELIVERY BY POLYMERIC NANOPARTICLES
Anti-diabetic medications, like insulin and glucagon-like peptide1(GLP-1) and its analogues are critical for diabetics to maintain blood glucose control. The difficulty of current insulin-based therapies for Type I & II diabetes mellitus, as well as the risks connected with fluctuations in blood glucose levels (hyperglycemia and hypoglycemia), served as the impetus for the establishment of "s...
AN OVERVIEW OF HERBAL COSMETICS AND THEIR APPLICATION
Plant or crude oriented herbs formulated products when they were application for their aromatic property in cosmetic formulation which known as or termed as herbal cosmetics. Now days it increasing demand for the herbal product has created new avenues in the cosmeceuticals market. Before some era, all cosmetics items were formulated in the house. Natural or herbs materials like aromatic materials,...
ANTIMICROBIAL ACTIVITY: DIFFERENT MECHANISTIC ELUCIDATION OF ANTIBACTERIAL, ANTIVIRAL, ANTIFUNGAL & ANTIALGAE AGENTS
In recent days, the increasing number of microbes and their increasing resistance power against conventional drugs have led to enormous worldwide mortalities, hence they pose a great threat to human health. The modern era is already going through the threat of COVID-19, also caused by one of those microbes called the virus. In order to get a clear understanding, all the microbes have been classifi...
ANTI-CANCEROUS ACTIVITY OF MEDICINAL PLANTS
The rising burden of cancer worldwide calls for an indispensable treatment result. Herbal drug provides a veritably doable volition to western drug against cancer. This composition reviews the named plant species with active phytochemicals, the animal models used for these studies, and their nonsupervisory aspects. This study is grounded on a scrupulous literature review conducted through the hunt...
EFFECT OF ACYRANTHUS ASPERA ON HIGH-FAT DIET AND STREPTOZOTOCIN INDUCE DIABETIC RATS: ROLE OF OXIDATIVE STRESS, PPAR-Α, GLUT-2 AND INSULIN SIGNALLING
Diabetes mellitus is a complex disorder where oxidative stress plays a crucial role in the development and progression of the disease. In the present work, the rat model evaluated pancreas and liver tissue damage caused by a high-fat diet and diabetic complications. Male Wistar rats were administered with HFD- corn oil orally for 28 days followed by streptozotocin (STZ) injection to induce diabete...
UV-B RADIATION INDUCED DIFFERENTIAL PHYSIOLOGICAL AND BIOCHEMICAL RESPONSES IN NOSTOC COMMUNE AND ANABAENA VARIABILIS
Batch cultures of two cyanobacterial species i.e., Nostoc commune and Anabaena variabilis were used to evaluate the impact of UV-B radiation on growth, photosynthetic pigments, lipid peroxidation, proline content, protein content as well as on the enzymes nitrate reductase and glutamine synthetase. The essential targets of UV-B radiation are the photosynthetic apparatus, photosynthetic pigments, a...
CARDIOPROTECTIVE EFFECT OF AESCIN ON ANTIOXIDANT AND LIPID PROFILE IN ISOPROTERENOL-INDUCED MYOCARDIAL INFARCTION IN RATS
The efficacy of aescin on the liver and cardiac markers, lipid profile, and antioxidant status in rats with myocardial infarction (MI) induced by isoproterenol (ISO) was investigated in this study. Three doses of aescin (5, 10, and 20 mg/kg of b.w) were administered to rats for the first 21 days. After the treatment period, ISO (60 mg/kg of b.w) was given subcutaneously to the rats on the 22nd and...
A STUDY ON THE HEPATOPROTECTIVE EFFECTS OF ETHYL ACETATE EXTRACT OF SPILANTHES ACMELLA IN PARACETAMOL-INDUCED HEPATOTOXICITY IN EXPERIMENTAL ANIMALS
Spilanthes acmella plant has been used for various purposes in traditional medicine since time immemorial. The present study aimed to determine the hepatoprotective activity of ethyl acetate extract of Spilanthes acmella in Paracetamol-induced hepatotoxicity in experimental animals. The leaves and flowers of this plant were used for this study, and the experiment was carried out in albino rats. Th...
OPTIMIZATION AND EVALUATION OF ANTIMICROBIAL COMPOUNDS PRODUCED BY STREPTOMYCES RUBRUS VLK-24
Optimizing the cultural conditions to enhance bioactive metabolite production by Streptomyces rubrus VLK-24 isolated from mangrove sediments as well as characterizing these compounds, has been taken up in this study. The culture broth inoculated in Yeast extract, Malt extract, and Dextrose broth and extracted with ethyl acetate exhibited high antimicrobial activity against test microorganisms, inc...
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF CYTARABINE AND DAUNORUBICIN AND THEIR DEGRADATION STUDIES IN BULK AND FORMULATION BY UPLC
The current investigation was Developing and validating a novel, simple, responsive, and stable UPLC method for the measurement of active pharmaceutical ingredients of Cytarabine and Daunorubicin. The chromato-graphic strategy utilized a phenyl column of dimensions 100 x 2.1 mm, 1.7 micron, using isocratic elution with a mobile phase of acetonitrile and 0.1 percent formic acid (70:30) with a flow ...
FORMULATION AND IN-VIVO EVALUATION OF MESALAMINE MICROSPONGES FOR COLON TARGETING
The present study aims to develop and characterize colon-targeted mesalamine microsponges for treating inflammatory bowel disease, requiring long-term therapy. Mesalamine microsponges were prepared with different ratios of eudragit S100, by quasi-emulsion solvent diffusion method and evaluated. The drug release from in-vitro dissolution for all the prepared microsponges was extended to 12 hrs and ...
HISTOARCHITECTURAL AND BIOCHEMICAL EVALUATION OF ISOPROTERENOL-INDUCED MYOCARDIAL ISCHEMIA IN HEART & LUNGS – AN EXPERIMENTAL DOSE-DEPENDENT STUDY
Background: Numerous methods have been used to induce myocardial ischemia (MI) in Experimental animals. MI induction using chemicals like isoproterenol in animal models is a simple and standardized method with fewer complications. Various doses of Isoproterenol were used extensively as an inducing agent. Aim: The present research aimed to evaluate the effect of Isoproterenol on the heart and lungs...
SCREENING OF ANALGESIC AND ANTI-INFLAMMATORY ACTIVITY OF VILAZODONE IN RODENTS
The analgesic activity of vilazodone in albino rats and mice was compared with the standard drug pentazocine and diclofenac, and its anti-inflammatory activity in albino rats was compared with the standard drug indomethacin. The doses of vilazodone used were 1.8mg/kg and 3.6mg/kg in albino rats and 2.6mg/kg and 5.2 mg/kg in albino mice, and the methods to screen analgesic activities were eddy’s ...
EFFECT OF QUERCETIN ON HYPOXIA-REGULATED METASTATIC MARKERS IN AGGRESSIVE PANCREATIC CANCER CELLS UNDER HYPOXIC CONDITIONS
Pancreatic cancer is an intractable and rare malignancy ranked 12th in incidence and 7th in mortality. It is the leading cause of cancer deaths in developed countries and is rising in developing countries like India. According to GLOBOCAN 2020 statistics, pancreatic cancer has ranked as the 11th most common malignancy in the world and accounts for 495773 new cases and caused 466003 deaths in 2020....
PRODUCTION AND CHARACTERIZATION OF BIOPOLYMER (PHB) USING BACTERIAL STRAINS ISOLATED FROM ALLUVIAL SOIL
Biopolymers are gaining much interest and are considered appropriate substitutes for petroleum-based plastics. It is used for various applications in the medical, agriculture, and packaging fields. The bacterial strains from river soil samples were isolated and screened for biopolymer production. The objective of the present study was to produce polyhydroxybutyrate (PHB) from the isolated strains ...
MOLECULAR-LEVEL COMPARATIVE ANALYSIS ON HUB-PROTEINS OF PARKINSON’S DISEASE WITH SELECTED SYNTHETIC DRUGS USING AN IN-SILICO APPROACH
Physical interactions between proteins are cardinal to biological processes. To restore their function, proteins must interact with one another. Hub proteins are densely interconnected proteins with a wide range of biological significance and are also to blame for several illnesses, including cancer, autoimmune disorders, and neurodegenerative diseases. The most prevalent of these is Parkinson's d...
PRECLINICAL SAFETY EVALUATION OF BERBERINE IN COMBINATION WITH GLICLAZIDE
Allopathic drugs are generally prescribed for Diabetes Mellitus (Type-II) patients. As the disease is chronic in nature, it necessitates the use of alternative medicine(s) either to supplement the existing allopathic therapy and/or to reduce the associated side effects with their chronic use. The use of herbal drugs is presumed to be safe option due to fewer side effects. However, evaluation of sa...
DRUG UTILIZATION EVALUATION OF ANTIBIOTICS IN INDIAN TERTIARY CARE HOSPITAL
Background: Infections occur when viruses, bacteria, or microbes enter the body and multiply. Control of infection is achieved by using antibiotics; hence scrutiny of antibiotic prescribing patterns is an important aspect of quality and standards of clinical practice. Objective: To evaluate and analyse drug utilization and prescription pattern of antibiotics in various in-patient departments of th...
IN-SILICO VALIDATION AND PHARMACOLOGICAL ACTIVITY OF POTENT ANTI VIRAL AND ANTI INFLAMMATORY ETHNO MEDICINAL PLANTS USED BY TRADITIONAL HERBALISTS WITHIN THORANGTLANG WILDLIFE SANCTUARY, MIZORAM, NORTH-EAST INDIA
Background: Our study aims to document the use of ethnomedicinal plants reported by herbalists in treating ailments that are becoming more prevalent. Using an ethnobotanical approach and in-silico studies, will advise further prevalence and give precedence to future pharmacological studies. Methods: The application of the plants was reported by 75 informants. From the 51 reported plants, 20 plants...
DESIGN AND CHARACTERISATION OF AMOXYCILLIN LOADED DENTICAP USING MUCOADHESIVE GUM ISOLATED FROM FRUIT PULP OF ZIZIPHUS MAURITIANA L.
Novel drug delivery approach by using natural polymers is a newer area of formulation development. The oral drug delivery in the buccal cavity to treat the dental infections has some limitations such as slow onset of actions and is also affected by the first pass metabolism. In order to overcome these challenges plant derived mucoadhesive gum from the fruit pulp of Ziziphus mauritiana L.(ZM) is us...
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE AND VALSARTAN IN ITS BULK AND TABLET DOSAGE FORM BY USING THE QUALITY-BY-DESIGN APPROACH
This study describes the implementation of a Quality by Design approach to develop and validate the RP-HPLC for amlodipine and valsartan. The reaction surface method optimizes by adopting the three-level Box Behnken design. The three factors selected are methanol and water concentrations (mobile phase), flow rate, and wavelength. The developed chromatographic method was validated against the ICH Q...
EVALUATION OF ANTI-NOCICEPTIVE EFFECT OF ETHANOLIC EXTRACT OF VANILLA PLANIFOLIA SEEDS IN ANIMAL MODELS OF PAIN
Background: Pain is warning signal of the underlying pathology and is also essential for the diagnosis. Vanilla planifolia (VP) as per literature has analgesic activity. Analgesics like NSAIDs and Opioids are used for treating pain. Adverse effects limits their long-term use. Therefore, there is always a need of more effective and safe drugs. Objective: To evaluate the analgesic activity of ethano...
A SIMPLE METHOD DEVELOPMENT, VALIDATION AND QUANTIFICATION OF LENVATINIB IN PHARMACEUTICAL DOSAGE FORM USING ZERO ORDER AND FIRST-ORDER DERIVATIVE NOVEL SPECTROPHOTOMETRIC METHODS
Simple, very Sensitive, rapid, and accurate novel spectro-photometric methods were developed to estimate Lenvatinib in Pharmaceutical dosage form using zero- and first-order derivative spectrophotometry. The solutions of Lenvatinib standard and sample were prepared using analytical grade methanol. The spectrophotometric estimation of lenvatinib was carried out at 240 nm in the zero-order method an...
QUANTITATIVE ESTIMATION OF PHYTOCONSTITUENTS AND ANTI-HISTAMINIC ACTIVITY OF QUISQUALIS INDICA LINN
The objectives of the present study were to determine the total phenolic content, flavonoid content, and anti-cataleptic activity of chloroform and methanolic extract of the roots of Quisqualis indica. The crude extracts were investigated for total phenolic and flavonoid content using the Folin-Coicalteu method and the Aluminum chloride colorimetric method. It was found that the total phenolic con...
EVALUATION OF PRELIMINARY TRIAL BATCHES OF FORMULATIONS CONTAINING ORAL HYPOGLYCEMIC DRUGS METFORMIN HYDROCHLORIDE AND CANAGLIFLOZIN
The in-vitro drug release profile from a hydrophilic matrix tablet is influenced by the viscosity of the gel layer formed due to its polymer hydration, and it depends on various other physical properties like a drug: polymer ratio water-solubility and particle size of the drug, particle size and type of the polymer, type of diluents used, and temperature of media. The drug release profile of diffe...
EFFECTIVENESS OF SIDDHA MEDICINE FOR THE MANAGEMENT OF ERYTHEMA DYSCHROMICUM PERSTANS (KARUNGUTTAM) – A CASE REPORT
A 30 years male patient from Chennai has presented with asymptomatic, grey-blue hyperpigmented patches of variable shape and size and an elevated erythematous border; the eruption has been symmetrically distributed on the face, the trunk, and the upper extremities for 4 years. This condition was diagnosed as “erythema dyschromicum perstans” which resembles to karunguttam one of the skin diseas...
FORMULATION DEVELOPMENT OF ORAL FAST-DISSOLVING SUBLINGUAL FILM OF RESVERATROL
This research aimed to develop an inclusion complex of resveratrol (RSV)/hydroxypropyl-β-cyclodextrin (HP-β-CD) using a quality-by-design (QbD) approach to improve the solubility and stability of RSV and formulate it into a fast-dissolving sublingual film (FDSF). The RSV/HP-β-CD inclusion complex was prepared by the kneading method using a Box–Behnken Design to assess the effect of the molar ...
METHYL BENZOATE FROM ACANTHASTER PLANCI EXHIBITS LIPID-LOWERING ACTIVITY IN HYPERCHOLESTEROLEMIC SPRAGUE-DAWLEY RAT’S HEPATOCYTE BY INCREASING SCAVENGER RECEPTOR CLASS B TYPE 1 (SR-B1) GENE EXPRESSION
Hypercholesterolemia occurs when a high cholesterol level in the blood may lead to atherosclerosis. Scavenger receptor class B type 1 (SR-B1) is a protein found on the surface of various cells that facilitates the reverse cholesterol transport or efflux of cholesterol from peripheral tissues back to the liver. SR-B1 receives cholesterol from high-density lipoprotein (HDL), which is then transporte...
ISOLATION OF ANTIBACTERIAL PEPTIDE FROM VIGNA ACONITIFOLIA L.
Vigna aconitifolia L. represents a genus in the Fabaceae family and is one of the most drought-resistant legumes, commonly grown in arid and semi-arid regions of India. Therapeutic effects of Vigna aconitifolia L. have been reported in several publications, but there are only a few reports on the biological activities of the Vigna aconitifolia L. proteins. In the present study, antibacterial a...
FORMULATION EVALUATION OF THE MULTIFUNCTIONAL PARABEN FREEHERBAL SHAMPOO FROM HOUSEHOLD HERBS AND COMPARISON WITH THE COMMERCIAL SHAMPOOS
The study's objectives were to create a pure herbal shampoo, assess its physicochemical qualities, and compare them to those of commercially available synthetic shampoos. The formulation of the herbal shampoo involved combining readily accessible at-home extracts of Reetha, Henna, Neem, Amla, almond powders, Alovera gel and tulsi lemon juice to a 10% aqueous gelatin solution. The pH was then corre...
COVID-19 AI STRUCTURAL MODEL TO MONITOR THE MULTIPLICATIVE NATURE OF COVID-19 INFECTIONS
In the present COVID-19 situation, it poses a danger to a person's life because of organ infection and other health problems. It is mandatory to research work to find a better COVID-19 infection diagnosis method through scans and contact tracing through the AI method. In this, a novel AI structural model is intended to identify the infection features in the respective regions of human being availa...
DEVELOPMENT AND VALIDATION OF STABILITY-INDICATING HPTLC METHOD FOR THE ESTIMATION OF DROTAVERINE HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORM
Drotaverine (DRO) is an anti-spasmodic medication inhibiting phosphodiesterase-4, an enzyme that breaks down cyclic adenosine monophosphate (cAMP). PDE4 inhibition causes an increase in cAMP, which causes smooth muscle relaxation. For DRO, especially the HPTLC technique and stability indicating studies through HPTLC method have not been reported properly in any database. The current research focus...
A COMPARATIVE STUDY OF KNOWLEDGE, ATTITUDE AND PERCEPTION OF JAUNDICE AMONG PARAMEDICAL AND NON-PARAMEDICAL STUDENTS
Jaundice is defined as the yellowing of the skin and whites of the eyes which are caused by a buildup of a substance called bilirubin in the blood and tissues in the body. Bilirubin is a yellowish pigment formed by the breakdown of heme, which occurs largely in hemoglobin and red blood cells. The purpose of this study was to analyze and compare the knowledge, attitude, and perception of jaundice a...
COMPUTATIONAL ANALYSIS OF PHARMACOKINETICS, BIOACTIVITY AND TOXICITY PROFILING OF SOME SELECTED OVULATION-INDUCTION AGENTS TREATING POLYCYSTIC OVARY SYNDROME (PCOS)
PCOS (Polycystic ovarian syndrome) is a prevalent female endocrine disorder with global occurrence ranging from 6-26%. It accounts for the majority of anovulatory infertility in women of reproductive age due to enlarged ovaries with small cysts formation, which the National Institute of Health has reported as a cause of 30% of infertility cases in females worldwide. Anovulatory infertility is gene...
PREDICTORS OF POLYPHARMACY PRESCRIPTION IN TYPE 2 DIABETES MELLITUS PATIENTS: A CROSS-SECTIONAL SURVEY
Polypharmacy is defined as the “prescription, administration, or use of more medications than are clinically indicated. It can lead to drug-drug interactions and result in adverse drug events. Therefore, this study aimed to determine the prevalence of polypharmacy and factors that independently predict polypharmacy prescription. 200 Type 2 Diabetes Mellitus (T2DM) patients were enrolled and anal...
INFECTIONS ASSOCIATED WITH BIOLOGIC THERAPY: A CLINICAL META-ANALYSIS STUDY
With the popularity of biologics, treatment for several inflammatory diseases has improved significantly. Biologics are a group of drugs obtained from biological sources that primarily function by suppressing part of the host immune system. However, this process comes at a cost, as it has been reported that the use of biologics carry a major risk in the form of tuberculosis. To verify this claim, ...
FIRST REPORT ON AMYLASE PRODUCTION BY BACILLUS PUMILUS DS5 ISOLATED FROM AGRICULTURAL FIELD SOILS
For the present study, 10 bacterial strains were isolated from agriculture field soils collected from Guntur, Andhra Pradesh, India, and thereafter screened for amylase activity. Among them, the strain DS5 showed a maximum zone of clearance on the starch hydrolysis test was selected for this study. Identification of bacterial strain DS5 was confirmed by 16S rRNA sequencing analysis. The phylogenet...
MOLECULAR PROPERTY AND TOXICITY PREDICTIONS AND EVALUATION OF ANTI-ARTHRITIC AND ANALGESIC ACTIVITIES OF L-ASCORBIC ACID DERIVATIVES USING IN-SILICO MODELS
Docking has become an indispensable method for predicting a molecule’s efficacy and toxicity. This technique is of great value in reducing the pain and suffering caused due to toxic drugs when tested in animals. Rheumatoid arthritis and the pain associated with the condition is a curse to mankind. In this research, the molecular property and toxicity predictions and evaluation of anti-arthritic ...
MOLECULAR DOCKING BASED IN-SILICO APPROACH REVEALS THE POTENTIAL INHIBITORY ACTIVITY OF NSAIDS AGAINST SARS-COV-2 PROTEINS
The rapid rate of mutation of the RNA genome of the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is responsible for the emergence of viral variants, leading to the enhanced survivability of the virus. Hence, searching for new drugs that can restrict new viral infections by interacting with wild-type and mutated viral proteins is important. However, new drug development's economic a...
FORMULATION AND EVALUATION OF POLY-HERBAL ANTI-DIABETIC FAST-DISSOLVING TABLET FROM HOME REMEDY AND COMPARISON WITH MARKETED METFORMIN TABLET
The current study's purpose was to develop and test a multi-herbal anti-diabetic quick-dissolving tablet compared to a commercially available metformin tablet. The multi-herbal anti-diabetic tablet contains natural medicines with anti-diabetic properties, such as Curcuma longa, Cinnamomum zeylanicum, Zingiber officinale, and Trigonella foenumgraecum. This mixture contains the botanical extracts Cu...
FORMULATION AND EVALUATION OF pH TRIGGER NASAL IN-SITU GEL OF LEVOCETIRIZINE HYDROCHLORIDE FOR LOCALIZED TREATMENT OF ALLERGIC RHINITIS
Objective: This dissertation work aimed to prepare Levocetirizine HCL (LCH) nasal in-situ gel, a second-generation antihistamine, to improve its retention time, sustain release, enhance the bioavailability, and avoid first-pass metabolism. Process: The in-situ nasal gel was prepared to consume Carbopol as a gelling agent, Hypromellose (HPMC) as a thickening or thickness-imparting agent, and buffer...
A SIMPLE AND SENSITIVE RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF HYDROXYCHLOROQUINE SULPHATE AND NITAZOXANIDE IN BINARY COMBINATION
The purpose of the present research was to develop a suitable, simple, precise, accurate, robust and reproducible RP-HPLC method for reliable simultaneous quantification of Hydroxychloroquine sulphate (HCQ) and Nitazoxanide (NTZ) in a synthetic mixture. The samples were assayed by the cyber lab HPLC instrument using a C18 (250mm, × 4.6mm, D., 5µm) –cyber-sil under isocratic conditions. The mob...
PRECLINICAL EVALUATION OF CROTON OIL FORMULATIONS FOR ITS HAIR GROWTH POTENTIAL
Traditionally, Croton tiglium seed oil is reported to be very effective for hair loss. Thus, the aim is to study the effectiveness of Croton tiglium with different oils in the treatment of alopecia. The study will help develop the pharmacological profile of herbal formulation, which is being reported scientifically for the first time. The herbal formulations (formulation1, formulation2, formulatio...
BIO ANALYTICAL VALIDATED TECHNIQUE FOR THE SIMULTANEOUS ESTIMATION OF AMLODIPINE, ROSUVASTATIN AND VALSARTANIN HUMAN PLASMA BY RP-HPLC METHOD
A reverse phase high-performance liquid chromatographic bio analytical Technique (RP-HPLC) was developed and validated as per ICH 2019, US FDA 2018 guidelines for the quantification of amlodipine besylate, Rosuvastatin and Valsartan in human plasma using RP - C18 column. The mobile phase in composition (acetonitrile: water in the ratio 75:25% v/v, pH adjusted to 4.0 with acetic acid) was pumped an...
ASSESSMENT OF MICROBIOLOGICAL QUALITY AND ORGANOLEPTIC CHANGE ON STORAGE DURATION OF STANDARDIZED COOKIES PREPARED BY BLEND OF LOTUS STEM POWDER WITH REFINED WHEAT FLOUR FOR DETERMINATION OF ITS SHELF LIFE
Incorporating the under-consumed super food ingredients rich in nutrients or functional compounds is a growing concern in healthcare product formulation. Health-ensuring cookies blend with Nelumbonucifera (Lotus) stem powder were standardized in the laboratory and found its acceptable form with comparatively higher acceptability and good flavour profile. Safety assurance is also a part of product ...