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IDENTIFICATION OF E.COLI NISSLE 1917 PROTEINS BY USING 2-D GEL ELECTROPHORESIS UNDER THE INFLUENCE OF COCOS NUCIFERA SAP AND WINE
Proteomics is the large scale study of proteins. Before going to analyse the differentially expressed proteins, through 2-D gel electrophoresis the proteins were extracted in a sample under stress. In the present study, we were isolated the protein samples of E. coli Nissle 1917 treated with cocoti sap and wine stress. The isolated proteins washed with 2-D clean up kit because it was very sensit...
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VIABILITY OF ADIPOSE-DERIVED STEM CELLS (ASCs) ON POROUS CHITOSAN SCAFFOLD
Background: Stem cells have great potential in regenerative medicine due to their multipotentiality. Treatments involving cell sheets for tissue reconstruction require a large number of cells to repair the tissue damage, and the cells are fragile. Combined with a porous chitosan scaffold (PCS), adipose-derived stem cells (ASCs), which are ubiquitous, can be delivered to a target tissue in vivo. Th...
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PHARMACOGNOSTICAL OVERVIEW ON TERMINALIA ARJUNA: A JUSTIFICATION OF FOLKLORIC BELIEF
The application of medicinal plants such as Terminalia arjuna to maintain health and treat disease started since time immemorial and still is a part of medical practice. The phytochemical analysis of bark of T. arjuna in methanolic, hydromethanolic (1:1), aqueous and petroleum ether extracts revealed the presence of carbohydrates, amino acid, alkaloids, saponins, tannins, flavonoids, terpenoids, g...
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AN EXPERIMENTAL STUDY TO EVALUATE ANTI-INFLAMMATORY ACTIVITY OF MURIVENNA IN WISTAR ALBINO RATS
Murivenna is a taila (oil) which is widely used in South-Indian practices for external application in many of the localized conditions such as trauma, inflammation of joint, fracture, joint dislocation, wound, etc. Taila (Oil) is considered best for vatahara action. Vata is the main dosha involved in shopha (inflammation); this is a suitable taila (oil) that works in the treatment of shopha (infla...
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AILANTHUS EXCELSA ROXB (MAHA NEEM)- AN HOLISTIC INSIGHT OF THE MULTIPURPOSE TREE
In recent years, Plants and herbal drugs obtaining from natural resources are again gaining importance throughout the world in maintaining the healthcare conditions of individuals. The regular use of natural sources and plant-derived products throughout the world has increased serious concerns over their quality, safety, and efficacy. Adding to the natural scientific resources and knowledge, the t...
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A STUDY OF NEUROBEHAVIORAL AND BIOCHEMICAL ACTIVITIES OF ACORUS CALAMUS LINN. ON RESTRAINT STRESSED WISTAR RATS
Life span of human, a gradually increase can occur with change in diet and life style which play an important role in delay or even block the progression of age related degenerative problems like dementia, alzheimer’s disease. Exposure to various stress is the emerging greatest challenge to the society recent years. The ill-effect of stress is release of oxidants which is found to be always asso...
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ETHNOBOTANICAL, PHYTOCHEMICAL AND PHARMACOLOGICAL SCIENCE OF TRACHYSPERMUM AMMI (AJWAIN) A SYSTEMATIC REVIEW
The aim of the present investigation was a systemic review of Trachyspermum ammi L. (Ajwain) coming from the family Apiaceae and its medicinal significance. The seeds of ajwain include approximate three-5% crucial brownish oil, and this species additionally incorporate thymol as the principal chemical constituent at the side of a few different important constituents like β-pinene, α-terpinene, ...
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DESIGN, FORMATION AND EVALUATION OF A CO-DRIED DIRECTLY COMPRESSIBLE EXCIPIENT “TSAG-15” AND ITS UTILIZATION IN THE FORMULATION OF A RAPID RELEASE-MOUTH DISINTEGRATING ASCORBIC ACID TABLETS
A research was conducted to design a directly compressible filler binder characterized with rapid release and mouth disintegrating. Acacia gum was obtained from local source and was purified using a standard method. Tapioca starch was also extracted from cassava tuber using a standard method. Both primary excipients were evaluated for power properties and coprocessed at varying concentration using...
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NEW STABILITY INDICATING UPLC METHOD FOR SIMULTANEOUS DETERMINATION OF DAPAGLIFLOZIN AND SAXAGLIPTIN
A new precise, selective, accurate and rapid Ultra Performance Liquid Chromatographic stability indicating method has been developed and validated for simultaneous quantitative determination of Dapagliflozin and Saxagliptin in bulk and pharmaceutical dosage form. The chromatographic separation was achieved with HSS 100 × 2.1 mm, 1.8 m size column. The optimized mobile phase consisting of Phosphat...
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DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF SAXAGLIPTIN AND DAPAGLIFLOZIN IN BULK AND DOSAGE FORM
Objective: In the present work, A Simple, rapid, sensitive, precise, and reproducible, specific UV spectrophotometric method for the determination of Saxagliptin (SAXA) and Dapagliflozin (DAPI) in bulk drug and pharmaceutical dosage form were developed and validated. Methods: A simple double beam UV spectrophotometric method has been developed and validated with different parameters such as linear...
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