Volume 9 (2018) - Issue 11, November
STUDY THE MOLECULAR MECHANISM OF SALVIA SPECIES IN PREVENTION OF DIABETES
Diabetes mellitus is a widespread epidemic caused by insulin deficiency, decreased secretion, or both of them. This disease is one of the common metabolic disorders that affects 8.2% of the world population and is expected to reach 4.5% in 2025. More than 200 species of plants have anti-diabetic properties, but the mechanism of their effect is not well defined. Most herbs that have anti-diabetic p...
A REVIEW ON THYMOL ENCAPSULATION AND ITS CONTROLLED RELEASE THROUGH BIODEGRADABLE POLYMER SHELLS
Encapsulation of thymol is important for its volatile nature, taste, and antispasmodic, antioxidant, antimicrobial, anticancer and anti-inflammatory properties. This review provides a summary of thymol encapsulation in different biodegradable polymers along with the methods of encapsulation and control release in various parts of the body. Biodegradability of shell material along with its health c...
A COMPREHENSIVE REVIEW ON THE USEFULNESS OF TELEHEALTH INTERVENTION FOR CHILDREN WITH DISABILITY WITH SPECIAL REFERENCE TO SAUDI ARABIA
Background: Healthcare requirements have changed gradually from the primitive ages to this modern era. Information and communication technology has opened innovative techniques like home healthcare for patients through telehealth use. Aims: 1. To find out the usefulness and effectiveness of telehealth intervention on the daily living activities amongst children with disability in Saudi Arabia. 2. ...
PLURONIC LECITHIN ORGANOGELS: AN EFFECTIVE TOPICAL AND TRANSDERMAL DRUG DELIVERY SYSTEM
Pluronic lecithin organogels (PLOs) are thermo-dynamically stable, biocompatible, viscoelastic with improve drug permeation and localised action. PLOs have gained much popularity compared with other traditional topical and transdermal drug delivery system owing to their lower cost, flexibility of dose and longer contact time. PLOs enhances the permeability of drugs because of desired drug partitio...
EVALUATION OF ANTIHYPERGLYCEMIC AND ANTIOXIDATIVE EFFECTS OF DILLENIA INDICA L. USING IN-VIVO APPROACHES
Dillenia indica (DI) is a traditionally used medicinal plant which is reported to exhibit wide range of pharmacological activities. However, no vast scientific study has been conducted in this regard. The present study was designed to investigate the antihyperglycemic, antihyperlipidemic and antioxidative activities of the Dillenia indica fruits. Aqueous and methanolic extracts of the fruits were ...
ANTIOXIDANT AND CYTOTOXIC ACTIVITIES OF SELECTED PLANT EXTRACTS AGAINST HUMAN NON-SMALL CELL LUNG ADENOCARCINOMA (A549), HUMAN COLON CARCINOMA CELLS (HCT116) AND CHINESE HAMSTER NORMAL OVARY CELLS (AA8)
Chemotherapy and radiation are the most prescribed methods of cancer treatments but these are highly toxic and also are carcinogenic thus alternative cures are being explored especially on the use of cytotoxic substances isolated from natural sources like plants known for their ethnomedicinal properties. Natural chemopreventive agents such as antioxidants isolated from plants are argued to be impo...
PRECLINICAL ACUTE AND REPEATED DOSE TOXICITY OF PUERARIA TUBEROSA (PTWE) ON CHARLES FOSTER RATS
Here we have carried out the acute and repeated dose toxicity study on tubers of Pueraria tuberosa water extract (PTWE). For acute toxicity study, rats were orally administered a single dose of 2000 and 5000 mg/kg body weight of PTWE. For repeated dose toxicity study on rats in one set, the different doses were given daily up to 28 days to make general observation and mortality rate. In another se...
SAFETY EVALUATION OF GALANGAL (ALPINIA GALANGA) EXTRACT FOR THERAPEUTIC USE AS AN ANTIMICROBIAL AGENT
Hexane extract of Galangal (Alpinia galanga) rhizome was evaluated for acute dermal, oral and intraperitoneal toxicities using OECD guidelines. The undiluted crude galangal extract showed negligible irritation on non-abraded skin of New Zealand white rabbits with 0.25 primary irritation index whereas the abraded skin of the rabbits showed irritation for all tested dilutions of galangal extracts; 0...
DEVELOPMENT AND EVALUATION OF ELASTIC NOISOMAL FORMULATION FOR MIGRAINE TREATMENT USING TRNA APPROACH
The approach of site specific topical delivery of Rizatriptan Benzoate (RBZ), used for migraine using the elastic carrier at posterior cervical region for sustained action was attempted in the present study. RBZ-entrapped elastic niosomes were prepared using the modified mechanical stirring method. The formulation was optimized using 23 full factorial designs. The type of edge activator (EA), Twee...
SYNTHESIS, ELECTROCHEMICAL AND ANTIMICROBIAL ACTIVITY OF SCHIFF BASE AND ITS NICKEL (II) COMPLEX
The present study is focused on the electrochemical behavior of glycine 4-bromo acetophenonimine (GBAPI) and its Ni(II) complex. In buffer solutions (Britton-Robinson universal buffer and phosphate buffer) of pH 3 - 9 range, using cyclic voltammetric technique. GBAPI is synthesized by the condensation of 4-bromo acetophenone and glycine amino acid (1:1 molar ratio). The complex is generated by the...
BROMELAIN PLUS PEROXIDASE FROM PINEAPPLE INDUCES APOPTOSIS VIA MITOCHONDRIAL DEPENDENT PATHWAY IN LYMPHOMA CELLS
Bromelain is a protease present in pineapple fruit and stem. It is reported that commercially available pure bromelain has anticancer, anti-inflammatory and other therapeutic effects. But there are few reports available on the biological activity of bromelain along with peroxidase which is present in pineapple fruit extract. The aim of the present study was to investigate the effect of bromelain w...
EFFECT OF GALANTAMINE WITH NEUROPHARMACOLOGICAL BENEFITS IN WISTAR RATS MODELS OF EPILEPSY AND BEHAVIOUR
Involvements of oxidative damage, deficiency of acetylcholine and cognitive impairment have been reported in the pathophysiology of epilepsy. It has been demonstrated that cholinesterase is degranulated at the site of a lesion it cause brain disorder leading to epilepsy. Cholinesterase inhibitor (Galantamine) inhibits the release of cholinesterase thus it benefits in epilepsy. So the aim of the st...
THE ANTIBACTERIAL ACTIVITIES OF SYZYGIUM AROMATICUM (L.) MERR. & PERRY AGAINST ORAL BACTERIA AND ITS ANTIOXIDANT AND ANTIMUTAGENIC ACTIVITIES
Plants are an important source of substances which are claimed to induce biological activities. Although there are a few studies on antimicrobial and antioxidant activities of this plant, antimutagenic activity has not been studied and there is no study in Turkey. Antibacterial activities of Syzygium aromaticum against oral pathogens have not been reported until today. The scope of this work was t...
DESIGN DEVELOPMENT AND EVALUATION OF GASTRO RETENTIVE FLOATING MICROSPHERES OF ATAZANAVIR SULFATE
The purpose of present study was to formulate the gastro-retentive floating microspheres of atazanavir sulfate to be retentive in the stomach for prolonged period of time. The gastro-retentive floating microspheres were prepared by double emulsion w1/o/w2 solvent evaporation technique and characterization for particle size, SEM, floating time, FTIR, DSC, and entrapment efficiency. The in-vitro dru...
FORMULATION AND EVALUATION OF MICROSPHERES BASED ORO-DISPERSIBLE ROXITHROMYCIN TABLETS USING CENTRAL COMPOSITE DESIGN
The aim of this study was to develop microspheres based orodispersible tablets of roxithromycin using the solvent evaporation technique. The microspheres were formulated in to orodispersible tablets using direct compression technique by central composite design. The objective of the study was to analyze the effect of concentration of eudragit RS100 (X1) and concentration of polyvinyl alcohol (X2),...
ANTIPLASMODIAL ACTIVITY OF EXTRACTS OF KHAYA SENEGALENSIS (DERS.) A. JUS (MELIACEAE) AND MELIA AZEDARACH L., PLANTS OF SENEGALESE TRADITIONAL MEDICINE
Two medicinal plants (Khaya senegalensis and Melia azedarach), frequently used by a large part of the Senegalese population and in particular that of the natural region of Casamance in the traditional treatment of malaria, were selected to study phytochemistry and to compare the antimalarial activity of the different parts used (leaves, bark, and seeds). The extraction of these drugs was carried o...
IN-VITRO ANTI-OBESITY EFFICACY OF SELECTED PLANTS IN 3T3-L1 CELL LINE
Background: Obesity is a confronting global issue with excess accumulation of body fat to an extent that decreases life expectancy. It is multifactorial in origin; genetic, environmental and pathophysiological. It leads to metabolic syndrome characterized by type 2 diabetes, cardiovascular diseases, fatty liver, hyperlipidemia etc. Objective: In this study, we have screened hydroalcoholic extracts...
CHEMICAL AND BIOLOGICAL STUDIES OF VALUE-ADDED CINNAMON PRODUCTS IN THE SRI LANKAN MARKET
Cinnamon commonly known as ‘Kurundu’ in Sri Lanka has been used as a spice since antiquity. It is obtained from the inner bark of several species of the genus Cinnamomum that belongs to the family Lauraceae. Among the cinnamon species, Cinnamomum verum (syn. Cinnamomum zeylanicum Nees) is native to Sri Lanka and is also known as “Ceylon” cinnamon or “true” cinnamon. Cinnamon produced i...
BIOCHEMICAL STUDIES ON THE EFFECT OF ETHANOLIC EXTRACTS OF TRICHOSANTHES DIOICA AND CLITORIA TERNATEA IN STREPTOZOTOCIN INDUCED DIABETIC MALE WISTAR RATS
To investigate the effect of ethanolic extracts of leaf and fruit of Trichosanthe dioica (T. dioica) and leaf of Clitoria ternatea (C. ternatea) on the biochemical parameters in streptozotocin (STZ) induced diabetic Wistar albino rats. Healthy male adult Albino rats of Wistar strain were randomly divided into eleven groups of six rats each were assigned into normal and diabetic groups (Group I to ...
EFFECT OF BISPHENOL-A ON THE CARDIOVASCULAR SYSTEM IN PREGNANT RATS
Bisphenol-A (BPA) is a chemical produced in large volumes worldwide and has large market diffusion in many consumer products. BPA is used mainly as a monomer in the production of polycarbonate plastics and as a precursor of epoxy resins. So the present study was undertaken to examine the toxic effect of BPA on heart function in pregnant rat models. BPA (50 mg/kg.b.wt/day and 500 mg/kg.b.wt/day) wa...
ANTI-INFLAMMATORY AND ANALGESIC ACTIVITIES OF PYROSTEGIA VENUSTA PRES. STEMS
Objective: Pyrostegia venusta (Ker Gawl.) Miers, Bignoniaceae, is native to the Brazilian Cerrado and popularly known as “cipó-de-são-joão”. In Brazilian folk medicine, the flowers of P. venusta are used as a general tonic and a treatment for diarrhea, vitiligo, cough, and common infections and inflammatory diseases of the respiratory system. Nevertheless, there are still no studies on its ...
DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPTLC METHOD FOR SIMULTANEOUS ESTIMATION OF LAMIVUDINE AND DOLUTEGRAVIR SODIUM IN BULK AND PHARMACEUTICAL DOSAGE FORMULATION
A sensitive, accurate, and precise stability-indicating HPTLC method has been developed for the simultaneous estimation of lamivudine (LMV) and dolutegravir sodium (DOL) in bulk and pharmaceutical dosage formulation. The method employed chloroform: methanol: toluene formic acid (8:2:2:0.2 v/v/v/v) as a mobile phase and silica gel G 60 F254 TLC plates as stationary phase. Chromatographic detection ...
SYNTHESIS, CHARACTERIZATION AND EVALUATION OF SCHIFF’s BASE MUTUAL PRODRUGS OF NAPROXEN
Background: A Schiff base, a pharmacologically privileged scaffold and a nitrogen analog of an aldehyde or ketone in which the C=O group is replaced by C=N-R group. Mutual prodrug is a Carrier linked prodrug consist of two pharmacologically active substances together in a single moiety. Objective: The goal of the study is to synthesize combined form of the various Schiff’s bases with Naproxen fo...
ASSESSING THE EFFECT OF PROCESSED NIGELLA SATIVA ON OLIGOMENORRHEA AND AMENORRHEA IN PATIENTS WITH POLYCYSTIC OVARIAN SYNDROME: A PILOT STUDY
Objective: The aim of this pilot study was to assess the effect of processed Nigella Sativa (N. Sativa) on oligomenorrhea and amenorrhea in patients with Polycystic Ovarian Syndrome (PCOS). Materials and Methods: Ten women diagnosed with PCOS, having oligo-amenorrhea, participated in this study. The National Institutes of Health (NIH)’s criteria were used for the diagnosis. The patients, between...
BERBERINE CHLORIDE INDUCED APOPTOSIS IN HEPG2 CANCER CELL LINE VIA PPAR GAMMA ACTIVATION AND COX2 INHIBITION BY REGULATING PROSTAGLANDIN E2 (PGE2) SIGNALLING PATHWAY
Berberine hydrochloride is an isoquinoline alkaloid isolated in the form of Berberine hydrochloride from the roots and stems of Berberis vulgaris, B. aristata, Coptidis rhizoma and other plant species. Berberine is well known for its antiproliferative and anti inflammatory activity since long time. The present study is carried out to study mechanism of inducing apoptosis by berberine hydrochloride...
FORMULATION AND DEVELOPMENT OF BOSENTAN LOADED ONCE A DAILY TABLET FOR PULMONARY ARTERY HYPERTENSION USING LIPID MATRICES BY 32 FULL FACTORIAL DESIGN
To avoid problems of conventional therapy of drug delivery and reduced dose, sustained release matrix tablet of Bosentan was prepared using lipid base material as matrices. Primary screening of polymer was done by selecting different lipid base materials like Compritol 888 ATO, Precirol ATO 5, Eudragit RSPO, Glycerly monostearate (GMS) and cetosteryl alcohol. All the batches were prepared by direc...
IN-VITRO ALPHA- AMYLASE INHIBITORY ACTIVITY, ANTIOXIDANT POTENTIAL, AND GC-MS ANALYSIS OF CREPE GINGER (COSTUS SPECIOSUS (J. KOENIG.) SM) LEAVES
The leaves of crepe ginger (Costus speciosus (J. Koeng.) Sm. are utilized by the Subanen tribe of Lapuyan, Zamboanga del Norte, Philippines to treat diabetes. However, the hypoglycemic potential of this plant is not yet well-established. Thus, the present study investigated the in-vitro alpha-amylase inhibitory and antioxidant activities of the leaves of C. speciosus. The evaluation of the alpha-a...
DETERMINATION OF As, Hg, Pb, Cd AND Al IN THE LIPSTICKS IN THE KINGDOM OF SAUDI ARABIA
Using inductively coupled plasma optical emission spectroscopy (ICP–OES) I determined the concentrations of heavy metals, arsenic (As), mercury (Hg), lead (Pb), cadmium (Cd) and aluminum (Al) element after digestion using microwave pressure for 32 different types from the most popular and widely sold lipsticks in Riyadh markets. Analysis was conducted for the samples of expensive and cheap brand...
DEVELOPMENT AND VALIDATION OF STABILITY – INDICATING RP-HPLC CHROMATOGRAPHIC METHOD BY FORCED DEGRADATION STUDIES FOR AZATHIOPRINE BY RELATED SUBSTANCES
Simple, rapid and reproducible stability-indicating methods were established for quantitative determination of azathioprine using a, phenomenex 250 mm × 4.9 mm C18, 5 µm, inertsil and UV detection at 240 nm. The isocratic elution was used to quantify the analyte and the mobile phase was acetate buffer: acetonitrile: methanol (30: 35: 35) was pumped at 1.0 mL/min. The method was linear between 10...
DEVELOPMENT AND VALIDATION OF STABILITY INDICATING UPLC METHOD FOR SIMULTANEOUS ESTIMATION OF SOFOSBUVIR AND VELPATASVIR IN TABLET DOSAGE FORM
The objective of the method was to develop a simple, rapid, sensitive, precise, accurate and validated Ultra Performance Liquid Chromatographic (UPLC) method for the simultaneous estimation of Sofosbuvir and Velpatasvir in tablet dosage form. Chromatographic separation was achieved on an acquity UPLC HSS C18, 2.1 × 100 mm, 1.8µ column with a mobile phase composed of orthophosphoric acid buffer a...
ACU-TENS STIMULATION AT BILATERAL ZUSANLI (ST36) ACUPOINT IN STREPTOZOTOCIN INDUCED DIABETIC RATS
The ancient medicine acupuncture was used to treat the diabetes and its complications. Our study was aimed to find out the hypoglycemic effect of Acu-TENS electrical stimulation at bilateral Zusanli (ST36) acupoint in streptozotocin (45 mg/kg/body weight/IP) induced diabetic rats. Diabetes was induced in male albino wistar rats with the administration of single dose of streptozotocin(45mg/kg/body ...
SYNTHESIS OF SILVER NANOPARTICLES USING SWERTIPUNICOSIDE AND ITS ACTIVITY ON HUMAN BREAST CANCER CELL LINE (MCF-7)
In the current study, synthesis of silver nanoparticles (AgNPs) using swertipunicoside was tested against human breast cancer cell line (MCF-7). The swertipunicoside was tested against the normal cell line such as vero cells to find out the toxicity of the compound. The synthesized AgNPs were characterized using UV-visible spectroscopy, X-ray powder diffraction (XRD), fourier-transform infrared sp...
PREVALENCE of AmpC β-LACTAMASE IN PLASMID RESISTANT GENE FROM UTI CLINICAL ISOLATES BY MOLECULAR TECHNIQUES
Objective: To detect the prevalence of Plasmid resistant AmpC β-lactamases gene in clinical isolates of gram negative organisms from UTI patients which produce resistance against multiple antibiotics. The gene coding for AmpC β-lactamases is also present in E. coli & Klebsiella species was not expressed because due to lack of promoter region, but the transfer of chromosomal genes to plasmids...
RELATION OF INSULIN RESISTANCE AND BMI WITH SEVERITY OF CORONARY ARTERY DISEASE IN PATIENTS WITH AND WITHOUT DIABETES MELLITUS
Currently, coronary artery disease is reported as the major cause of mortality and morbidity and is expected to be the reason for 40% death in 2020. Since its rate is high in type 2 diabetes mellitus patients, a study has been designed to analyze the correlation of insulin resistance and obesity with incidence and severity of disease. Study was done on 109 individuals with established CAD in the a...
NEUROPROTECTION OF ETHANOLIC EXTRACT OF WITHANIA SOMNIFERA AND WITHANOLIDE A IN MOTOR CO-ORDINATION ON EXPERIMENTAL HUNTINGTON’S RAT MODEL
Neurodegeneration is a powerful initiator for a number of nervous system related diseases that come under the term “neurodegenerative diseases”. Neurodegenerative diseases are incurable, results in the progressive degeneration of nerve cells and causes problems with movement and mental functioning that became a challenge in present day’s health care. For this study we made a model of Hunting...
SIMULTANEOUS ESTIMATION OF HYDROCORTISONE AND KETOCONAZOLE IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD
A simple, selective, rapid, precise and economical reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of hydrocortisone and ketoconazole from pharmaceutical formulation. The method was carried out on a C18 (25 cm × 4.6 mm i.d., 5 μ) column with a mobile phase consisting of methanol: water (adjusted to pH 3.0 using triethylamine) in the...
ISOLATION AND PURIFICATION OF AN EXTRACELLULAR PROTEASE FROM BACILLUS SUBTILIS ASASBT ISOLATED FROM TERMITE SOIL
Proteases are one of the most important enzymes in many industrial applications, particularly as additives in laundry detergent industry. Hence, the present study investigates the isolation and purification of an extracellular protease from the Bacillus subtilis ASASBT isolated from termite soil sample. Among the five protease producers, one of the isolates was selected for further studies. It w...
INAPPROPRIATE NUTRITIONAL INTAKES DURING RAMADAN IN OBESE WOMEN PATIENTS WITH FATTY LIVER PRECLUDE ITS BENEFICIAL EFFECT
Introduction: Ramadan is a lunar month in the Islamic calendar. During this month, individuals who desire to keep a strict fast from dawn to sunset prevent stop eating and drinking. We evaluated the dietary intakes of patients with fatty liver during this month. Material and Methods: Sixty eligible overweight and obese women with fatty liver diagnosed via ultrasonography who had referred to the ma...
QUALITY ASSESSMENT OF DIFFERENT BRANDS OF MANGO JUICE AVAILABLE IN INDIAN MARKET FOR CARBOHYDRATES AND ACIDS (ASCORBIC ACID) BY CONVENTIONAL TITRATION METHOD
Fruits play an important role in human life because it provides valuable nutrients such as carbohydrates protein and fibres. Fruits are commercially processed to produce fruit juices, jams, jellies etc. Fruit juices sometime naturally contaminates with certain substances like carbohydrates, heavy metals etc. However, sometime manufacturer intentionally adds some substance like carbohydrates and ac...
A STUDY OF NEUROBEHAVIORAL AND BIOCHEMICAL ACTIVITIES OF ACORUS CALAMUS LINN. ON RESTRAINT STRESSED WISTAR RATS
Life span of human, a gradually increase can occur with change in diet and life style which play an important role in delay or even block the progression of age related degenerative problems like dementia, alzheimer’s disease. Exposure to various stress is the emerging greatest challenge to the society recent years. The ill-effect of stress is release of oxidants which is found to be always asso...
STANDARDIZATION OF AMRITAPRASHA GHRITA: A HERBAL GHEE BASED MEDICINAL PREPARATION
Aim: To standardize Amritaprasha ghrita. Materials and methods: Physico‐chemical studies like refractive index, specific gravity, acid value, saponification value, iodine value, determination of unsaponifiable matter, peroxide, viscosity, rancidity test and HPTLC were carried out as per the WHO guidelines, Indian Pharmacopoeia and Ayurvedic Pharmacopoeia. Conclusion: Standardization tests done...
APPLICATION OF EXTRACT OF GERANIUM PALUSTRE HERB AS A DENTAL FILM IN THE TREATMENT OF ORAL MUCOSA DISEASES
Investigation of antioxidant, anti-inflammatory and antimicrobial activity of a dental film, which easily reabsorbs with applied phytocomplex of Geranium palustre, has been performed to use it for the treatment of oral mucosa diseases. To get the extract, the dried material was grounded, extracted and lyophilized. Quantitative determination of biologically active substances in lyophilized phytocom...
PHARMACOGNOSTICAL, PHYTOCHEMICAL SCREENING AND EVALUATION OF ANTIOXIDANT ACTIVITY OF ETHANOL AND AQUEOUS EXTRACTS OF RANDIA SPINOSA LEAVES
Randia spinosa (Rubiaceae family), commonly known as ‘Mainphal’ is a large shrub or small tree. The plant grows in Brazil, Ceylon, China, East tropical Africa, India, Malaya and Sumatra. The leaves of Randia spinosa is used in traditional medicine to treat diarrhea and dysentery, snake bite, wound healing, inflammation, tumors, piles, gastrointestinal and skin diseases. The objective of this s...
PHYTOCHEMICAL SCREENING, QUANTITATIVE ANALYSIS OF FLAVONOIDS AND MINERALS IN ETHANOLIC EXTRACT OF CITRUS PARADISI
Like most other plants Citrus paradisi contain various secondary metabolites with great potentials. The aim of this paper is to evaluate the phytochemicals by using quantitative and qualitative analysis of ethyl acetate, ethanol, n-hexane and aqueous extracts with the help of standard techniques. The findings from quantification and phytochemical screening showed the presence of alkaloids, flavono...
PRODUCTION OF BIOSURFACTANT FROM BACILLUS SP. AND ITS LARVICIDAL ACTIVITY
Mosquitoes are becoming a serious threat to the humans causing infectious diseases like dengue, malaria, chikungunya, yellow fever etc. They multiply in large numbers in a polluted environment and stagnant water bodies. The control measures include the use of pesticides and chemical compounds which further causes harmful effects. Thus, an alternative solution is to use a biologically active compou...
PRODUCTION AND CHARACTERIZATION OF CURDLAN FROM AGROBACTERIUM SP.
Biopolymers are gaining more importance in our day-to-day life, due to the hazardous effect of synthetic plastics in our environment. Curdlan is one of the biopolymers, which has a broad field of applications in the food, pharmaceutical and medical industries. It is a polymer of glucose, produced by the family members of Rhizobiaceae. Curdlan [(1→3)-β-D-glucan] production takes place under the ...
RP-HPLC METHOD FOR ESTIMATION OF ROSUVASTATIN CALCIUM FROM BULK AND TRANSDERMAL DOSAGE FORM
A RP-HPLC method was developed for estimation of rosuvastatin calcium and validated as per ICH guidelines. The estimation was carried out on C-18 Reliant (4.6 × 250 mm, 5 µm) column using a filtered and degassed mixture of acetonitrile: phosphate buffer pH 2.6 (in the ratio 70:30) as the mobile phase at a flow rate of 1.0 ml/min. The detection was carried out at wavelength of 241 nm. The method ...
NEW VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF EMPAGLIFLOZIN IN HUMAN PLASMA
A new reversed phase simple, economic and specific validated high performance liquid chromatography method for the estimation of empagliflozin in human plasma is presented. A chromatographic separation was carried out isocratically by mobile phase comprising of methanol and acetonitrile, 50:50% v/v at a flow rate of 1.0 ml/min on agilent long C18 column (250 mm × 4.6 mm, 5 µm) with photodiode ar...
ANTIMICROBIAL ACTIVITY OF ALOE VERA GEL AND HONEY AGAINST BACTERIA ISOLATES FROM WOUND ASPIRATES
Wounds may harbour diverse microorganisms, especially bacteria that are resistant to many conventional antibiotics. The aim of this study is therefore to evaluate the antibiotic activities of Aloe vera gel and honey against bacteria isolates from wounds. A cross sectional study of wound aspirates from a health care center was carried out to evaluate the antibacterial activities of Aloe vera and ho...
ANTIOXIDANT EFFICIENCY OF VARIOUS EXTRACTS OF SEED COAT AND COTYLEDON OF CAJANUS CAJAN (L.) MILLSP.
Cajanus cajan L. (Pigeonpea) is an important pulse crop of India. The aim of this study was to determine and compare the antioxidant activity and bioactive compounds of seed coat and cotyledon of C. cajan. The antioxidant properties of pet ether, chloroform, ethanol and aqueous extracts of the seed coat and cotyledon of C. cajan were examined by a ferric thiocynate (FTC) assay, DPPH radical scaven...
ANALYSIS OF LIVER FUNCTION TESTS AND RENAL FUNCTION TESTS IN MAJOR DEPRESSIVE PATIENTS ON IMIPRAMINE, SERTRALINE AND ESCITALOPRAM THERAPY: AN OBSERVATIONAL STUDY IN A TERTIARY CARE HOSPITAL, AJMER, RAJASTHAN
Depression is the major cause of disability Worldwide. Antidepressant medications remain a mainstay of treatment for major depressive disorder. Our aim in this study was to analyze the LFT & RFT in patients of major depressive disorder taking imipramine, sertraline and escitalopram. A total of 810 such patients meeting the inclusion and exclusion criteria were randomly divided into three group...
IN-VITRO ANALYSIS TO ANALYSE THE DISINTEGERATION PROPERTY OF PRF WHEN TREATED WITH VARIOUS CONCENTRATION OF TRANEXAMIC ACID
Aim: To assess whether Tranexamic acid has any effect in inhibiting degradability of PRF membrane and also to compare the degradability of PRF at different concentrations of Tranexamic acid (200 mg, 150 mg and 50 mg). Material and Methods: The degradability of PRF membrane was compared between various concentrations of Tranexamic acid such as 200 mg, 150 mg, 50 mg against negative control of phosp...
HPLC STANDARDIZATION OF ETHYL ACETATE EXTRACT OF FAGOPYRUM ESCULENTUM MOENCH. SEEDS AND IN-VITRO ENZYME INHIBITORY ACTIVITIES
Fagopyrum esculentum Moench. is a versatile herb as beyond its nutritional properties it also have various health benefits like anti-diabetic, anti-inflammation, anticancer, antibacterial, anti hypertensive etc. Most of these activities can be attributed to the presence of certain bioactive components categorised as polyphenols, present in its various parts. Present work describes a method for sim...
ANTILITHIATIC ACTIVITY OF ETHANOLIC LEAVES EXTRACT OF DALBERGIA SISSOO LINN. ETHYLENE GLYCOL INDUCED LITHIASIS IN RATS
Aim of the Study: The aim of this study was to investigate the antilithiatic activity of ethanolic leaves extract of Dalbergia sissoo Linn. ethylene glycol induced in rats. Materials and Methods: All the chemicals were analytical grade. Cystone was obtained from from Himalaya Health Care Ltd. The ethanolic extract of plant Dalbergia sissoo Linn. leaves were given with tween 80 as vehicle administe...
QUALITY EVALUATION OF FOUR BRANDS OF MALE CONDOMS MARKETED IN ADDIS ABABA, ETHIOPIA
Condom is usually made up of thin rubber (latex) designed or is made of polyurethane to cover the penis sheath at closed one end that is inserted intravaginally during sexual intercourse for contraceptive purpose or as a means of preventive sexually transmitted diseases. Condoms should be safe, effective, user-controlled, non-prescription, easy to use and relatively cheap. The quality of the four ...
STANDARDIZATION OF DASHAMOOLA KWATHA CHOORNA: A HERBAL COMPOUND DRUG USED FOR PARISHEKA IN SPASTIC CEREBRAL PALSY
Background: Dashamoola is a group of ten drugs i.e. Bilva (Aegle marmelos (L.) Corrêa), Patala (Stereospermum suaveolens (Roxb.) DC.), Agnimantha (Clerodendrum phlomidis L.f.), Shyonaka (Oroxylum indicum (L.) Benth. ex Kurz), Gambhari (Gmelina arborea Roxb.), Bruhati (Solanum nigrum L.), Gokshura (Tribulus terrestris L.), Kantakari (Solanum xanthocarpum Schrad. & H. Wendl.), Prishniparni (Ura...
THE PRACTICE OF SELF MEDICATION IN DIARRHEA AMONG SECOND YEAR MEDICAL STUDENTS IN A TERTIARY CARE HOSPITAL IN JHARKHAND
Over the counter (OTC) drugs are drugs easily available without the prescription of a registered medical practitioner (RMP) and self-mediation practices of these drugs are very common in medical students especially in diarrhea which mostly requires dehydration therapy only. But very few reports are available regarding the use of OTC antidiarrhoeal drugs. The aim of the study was designed to assess...
SYNTHESIS, CHARACTERIZATION, INDUSTRIAL APPLICATION AND ANTICANCER ACTIVITY OF NEW N-MUSTARD SUBSTITUTED COUMARIN DERIVATIVES
A series of N-mustard substituted coumarin have been synthesized characterized and evaluated for their in-vitro cytotoxicity and anticancer activity against PC-3 human cancer cell lines. The methodology involves nucleophilic substitution of readily accessible 3-cyano-4-chloro coumarin with ethanol amine further on chlorination gives substituted 4-(bis(2-chloroethyl)amino)-2-oxo-2H-chromene-3-carbo...
INCIDENCE AND PATTERN OF ADVERSE DRUG REACTIONS (ADRs) IN PATIENTS TREATED FOR TUBERCULOSIS UNDER DOTS AT A TERTIARY CARE HOSPITAL OF NORTHERN INDIA
Background: WHO statistics for 2015 give an estimated incidence figure of 2.2 million cases of TB for India out of a global incidence of 9 million thus making India accountable for almost one-third of the global TB burden. The first-line antitubercular drugs include H (Isoniazid), R (Rifampicin), Z (Pyrazinamide), E (Ethambutol) and S (Streptomycin). The treatment of tuberculosis being of longer d...
EFFECT OF COMBINED EXPOSURE OF DICHLORVOS AND MONOCROTOPHOS ON NEUROTRANSMITTERS AND ACETYLCHOLINESTERASE IN RAT
Organophosphate pesticides are associated with neurotoxicity. Organophosphates are cholinesterase-inhibiting chemicals used predominately as pesticides. These have played a very important role in sustainable production of food, animal feed and as protection against disease vectors. They are also used as chemical warfare agents (nerve agents). The effects of dichlorvos and monocrotophos on brain ne...
COMPARE THE EFFICACY AND SAFETY OF DIPEPTIDYL PEPTIDASE-4 INHIBITORS WITH OTHER ORAL HYPOGLYCEMIC AGENTS IN TYPE 2 DIABETES MELLITUS PATIENTS
Background: The incidence of Diabetes Mellitus has increased dramatically in recent decades. Di Peptidyl Peptidase 4 inhibitors (DPP-4) have their role in glycemic control. An impaired ‘incretin effect’, occurs in patients with type 2 Diabetes Mellitus in response to glucose intake. Objectives: The main objective of the study is to compare therapeutic outcomes and adverse drug reactions among ...
PERFORMANCE IMPLICATIONS OF CLINICAL PHARMACIST INFORMATION ON GESTATIONAL DIABETES MELLITUS AT A TEACHING HOSPITAL IN SOUTHERN INDIA
Context: Gestational diabetes mellitus (GDM) early diagnosis of disease can reduce the morbidity and mortality by prevention of maternal and fetal complications. Aims: To assess the impact of clinical pharmacist knowledge on GDM mothers. Setting Tertiary care teaching hospital of North Karnataka, India. Methods and Materials: A randomized controlled cross-sectional study was carried out for a peri...
IN-VITRO EVALUATION OF ORODISPERSSIBLE FILM OF ALLIUM SATIVUM LINN.
The treatment of cancer up to the saving of life of cancer patient is rare miracle. Cancer is deadly disorder (some cancers are diseases caused by viruses) if diagnosed in second or third stage leads to death, hence it is better to prevent it. The present study emphasizes on prevention of cancer rather than cure. Extraction of garlic was carried out by using maceration process. The liquid extract ...
SCREENING OF SULPHUR OXIDIZING BACTERIA FROM SULPHUR SPRINGS OF SAHASTRADHARA REGION OF DEHRADUN, UTTARAKHAND, INDIA
Sulphur water is commonly used as the remedial source for treating skin infections and eradicating pathogenic microbes causing infection. The sulphur water is the source of sulphur oxidizing bacteria which is the source of different secondary metabolites which has potential killing activity against different skin borne infections. In the present investigation, the study was performed to isolate an...
ANALYTICAL QUALITY BY DESIGN APPROACH IN RP-HPLC METHOD DEVELOPMENT FOR THE ASSAY OF PITAVASTATIN IN TABLET DOSAGE FORM
Chromatographic method was developed according to Quality by Design (QbD) approach as per ICH Q8 (R2) guidelines for estimation of pitavastatin in pharmaceutical dosage form. By considering the current regulatory requirement for an analytical method development, a reversed phase high performance liquid chromatographic method for routine analysis of Pitavastatin has been optimized using analytical ...
ANTIOXIDANT ACTIVITY ASSESSMENT OF CAMELLIA SINENSIS LEAVES EXTRACT IN GUINEA PIG PLASMA
This study planned to screen preliminary phytoconstituents, total phenolic- flavonoids content and in-vitro, in-vivo antioxidant activities of aqueous crude extract of Camellia sinensis leaves. We assessed phytochemical constituents in extract using previously reported methods for tannins, phenols, flavonoids, glycosides, alkaloids and saponins. Quantitative estimation of flavonoids and phenolic c...
ASSESSMENT OF SURGICAL STRESS RESPONSE AND PREDICTING ITS FUNCTIONAL IMPLICATIONS
Surgery alters the homeostatic balance and defence mechanisms in body eliciting certain responses called as stress response. In addition certain peri-operative factors may also influence the degree of stress response reflecting surgical recovery. The present study is an attempt to assess surgical stress response and to determine the influence of peri-operative factors on it. This study investigate...