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CHROMATOGRAPHIC METHODS OF FRACTIONATING SEVERAL CHEMICAL CONSTITUENTS IN PHYLA NODIFLORA
Phyla nodiflora Linn., Synonym- Lippia nodiflora Rich also known as Jala-pippali is an important herb which can be used medicinally.The aim of the present study is to fractionate several chemical constituents in Phyla nodiflora. Chromatography was used for designing of multi component solvent system for different extracts where, for methanolic extract Hexane: Toluene: Ethyl acetate (2: 1.5: 0.5); ...
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PARTIAL PURIFICATION OF LIPASE FROM STREPTOMYCES VARIABILIS NGP 3 AND ITS APPLICATION IN BIOREMEDIATION OF WASTE WATER
Partial purification and bioremediation of waste water by lipase from the marine actinomycete Streptomyces variabilis NGP 3 (Accession no: (JX843530)) were carried out. The optimum incubation period, pH, temperature and agitation speed for enzyme production were fifth day (61.2 U/ml), 9.0 - 9.5 (105 U/ml), 35ºC (39.4 U/ml) and 120 rpm (38.7 U/ml) respectively. Lactose (2.0 g/l) and peptone (0.6 a...
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CARDIOPROTECTIVE EFFECT OF MORUS ALBA L. LEAVES IN ISOPRENALINE INDUCED RATS
The study was designed to evaluate the cardioprotective effect of methanolic extract of Morus alba L. leaves against isoprenaline- induced myocardial infarction and was investigated by an in vivo method in rats. Male Wistar albino rats were divided into four groups (n=6). Group I rats served as normal control. Group II rats served as isoprenaline induced toxic control (110 mg/kg body weight) which...
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PHARMACOGNOSTICAL EVALUATION AND PHYTOCHEMICAL ANALYSIS OF DELONIX REGIA RAFIN. STEM BARK
Delonix regia Rafin. belonging to family Fabaceae and subfamily Caesalpinioideae is flowering plant native to Madagascar and East Africa. Delonix regia reported to have anti-diarrhoeal, anti-inflammatory, antioxidant, hepatoprotective and antimicrobial activity. The present study was carried out to establish the pharmacognostical studies, physico-chemical parameters along with preliminary phytoche...
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ANOVULATORY FOLLICLE DEVELOPMENT IN ADULT ALBINO MICE DUE TO PROLONGED EXPOSURES TO GENISTEIN
Genistein is a soy phytoestrogen that mediates its functions by interacting with the endogenous estrogen receptor β. To investigate the effect of 10mg/kg body weight/ day genistein administration up to 90 days on the ovarian folliculogenesis and its correlation with serum gonadotropins and estrogen, adult Mus musculus were either administered with 1:4 DMSO : PBS (vehicle) or with 10mg genistein/k...
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IN-VITRO CYTOTOXIC ACTIVITY OF METHANOLIC EXTRACT OF HEMIDESMUS INDICUS R.BR.
Natural products represent a reservoir of diverse templates and are being tapped to outsource novel anticancer agents. Hemidesmus indicus R.Br.(Fam: Asclepidaceae) has been reported to be useful for the treatment of inflammation, cuts, wounds, burns, snake bite, skin and blood diseases, ulcers, immunological disorders . In the present study, the methanolic extract of the roots of Hemidesmus indicu...
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GC MS ANALYSIS AND ANTIMICROBIAL ACTIVITY OF SEED OIL OF BROUSSONETIA PAPYRIFERA (L.) VENT
The current study focuses on evaluating chemical composition and antimicrobial activity of seed oil of Broussonetia papyrifera; a widely used Chinese medicinal plant. The chemical constituents of the oil were evaluated by GC‑MS analysis. Antimicrobial activity and minimum inhibitory concentrations were determined by disk diffusion and agar dilution methods. The seed oil contains 46 different p...
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PROTECTIVE PROFILE OF HYDNOCARPUS LAURIFOLIA ON STREPTOZOTOCIN INDUCED OXIDATIVE STRESS IN RATS
The present study was aimed to evaluate the anti-oxidative defence system of Hydnocarpus laurifolia in diabetic rats and its potential effect against the β-cell damage induced by oxidative stress. Hydnocarpus laurifolia is a plant which belongs to the family Flacourtiaceae. According to the Indian system of traditional medicine it is used in the treatment of diabetes mellitus. Also literature rev...
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SENSITIVE SPECTROPHOTOMETRIC DETERMINATION OF TRACE AMOUNTS OF VANADIUM (IV, V) USING DITHIOLPHENOLS AND HYDROFOB AMINS
Dithiolphenole (DP) has been synthesized and characterized with IR and NMR spectroscopic methods. A simple and spectrophotometric method has been developed for the determination of trace amounts of vanadium (IV, V). The reagents forms blue coloured insoluble in water complex with vanadium (IV, V) in a weakly acidic and slightly alkaline medium (pH 2.5 - 8.5). In the presence of hydrophobic a...
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EXPLORING IN-VIVO AND IN-VITRO OXALIS CORNICULATA L. FOR PHYTOCHEMICALS USING NON-TARGETED LC-MS APPROACH AND ITS ANTIOXIDANT CAPACITY
Oxalis corniculata Linn. (Oxalidaceae) is the nutraceutical important medicinal plant containing essential nutrients like sodium, potassium, calcium, nitrogen and magnesium and also gives health protection due to the presence of secondary metabolites of therapeutic importance. Present study highlights the phytochemical constituents present in the methanolic extract of in-vivo and in-vitro O. corni...
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