Volume 7 (2016) - Issue 7, July
Herbs & its formulations have a long history of use in the treatment of human diseases. Herbal extracts have long been regarded as a source of new and useful pharmaceuticals. According to Cragg's investigation, approximately 62 % of commercially available drugs have natural product origins. Manjistha, or Rubia Cordifolia to give it its scientific name, is a branched climber with small, green...
In this review our main focus is on flavonoid groups which are one of the main active constituents found with other active constituents like saponins and polysaccharides, while poisonous groups are nitro-compounds, indolizidine alkaloids and the seleniferous derivatives in Astragalus genus. It is well established fact that flavonoid possess many biological activity such as antiallergic, anti-infla...
Biomaterials can be thought as the key ingredients in several implants such as those used for joints, sutures, bone plates, and medical devices such as blood tubes, artificial heart, pacemakers, etc. They function by replacing, restoring the injured or destroyed tissues or organs, and thereby help in providing better essence of life of the patients. A biomaterial which is used in making implants s...
Microsponge is recent novel technique for control release as well as target specific drug delivery system. Therefore many researchers are attracted towards the microsponge drug delivery system. Microsponge technology has been introduced in topical drug products to increase the controlled release of active drug into the skin in order to reduce entry of drug in blood and maintain drug on skin surfac...
BIOTECHNOLOGICAL DESIGN TO OPTIMIZE THE PRODUCTION OF BIOACTIVE LACTOBACILLUS PLANTARUM BY-PRODUCTS: NOVEL CHRONIC WOUND TREATMENT
Lactobacillus plantarum culture supernatants (LAPS) have antimicrobial, pro‐healing and anesthetic properties, so our medical team applied whole cultures and LAPS in chronic wounds with encouraging results. Metabolites responsible for LAPS properties and its action mechanism were previously determined. Objective: to design methods and culture media to increase the production of LAPS metabolites ...
PHYTOCHEMICAL INVESTIGATION FOR THE WOUND HEALING POTENTIAL OF A NOVEL COMPOUND ISOLATED FROM MENTHA PIPERITA L. LEAVES
Mentha piperitais currently one of the most economically important aromatic and medicinal crops in all over the world. Phytochemical investigation of the leaves of Mentha piperita (L.) (Lamiaceae) yielded a new compound, which is decarboxyrosemarinic acid galactoside. Formulate as 3’, 4’-dihydroxy-β-phenyl ethyl caffeate-4-(3’’’-menthyl)-4’-β-D-galacto pyranoside. 13C NMR, 1H NMR, FA...
Azetidin-2-one analogues are reported to exhibit various pharmacological activities like cholesterol absorption inhibitory activity, human tryptase, thrombin and chymase inhibitory activity, vasopressin V1a antagonist activity, antidiabetic, anti-inflammatory, antiparkinsonian and anti-HIV activity in addition to antimicrobial.1-6 In the present study, Isoniazid (INH), the established antituberc...
CELL SUSPENSION OF SPIRULINA PLATENSIS PARTIALLY ATTENUATES ALLOXAN INDUCED ALTERATIONS IN CARBOHYDRATE AND LIPID METABOLISM IN DIABETIC MICE
The present study evaluates the hypoglycemic and hypolipidemic effects of supplementation of cell suspension of Spirulina platensis (SP) in alloxan (AXN) induced hyperglycemic mice. Diabetes was induced by intra-peritoneal administration of AXN. Mice with blood glucose level above 200 mg/dl were divided into Group I (control), Group II (diabetic control), Group III (diabetic control mice fed with ...
Background: In present study, we aim to assess the anticandidal activity of crude extracts and screening of bioactive compounds and their identification by NMR, IR and GC-MS. Results: A total of 192 isolates were isolated from patients having oral and vaginal candidiasis and a total of 134 isolates showed positive results with Gram staining. The selected isolates APKU-2, APKU-3 and APKU-4 were ide...
COMPARATIVE PHARMACEUTICAL QUALITY CONTROL TESTING OF DIFFERENT BRANDS OF PARACETAMOL TABLETS AVAILABLE IN THE TRINIDAD & TOBAGO, WEST INDIES
Paracetamol tablets are popular over the counter (OTC) products among the patients as a good analgesics and antipyretics. The objective of this study was to compare the quality of the paracetamol tablet formulations those are locally available in Trinidad & Tobago pharmaceutical market manufactured by various pharmaceutical companies with pharmacopoeia standards. The four popular brands (A, B,...
A SIMPLE, QUICK, REPRODUCIBLE AND LOW-COST HIGH PERFORMANCE LIQUID CHRO1MATOGRAPHIC METHOD FOR THE ANALYSIS OF METRONIDAZOLE IN TABLETS
A simple, rapid, reproducible and low cost High Performance Liquid Chromatography (HPLC) method was developed and used to assess the quality of some brands of metronidazole tablets being marketed in Abuja, Nigeria. Parameters such as assay for the content of active ingredient, dissolution, disintegration, hardness, friability, and microbial load were evaluated using the methods described in the Br...
EVALUATION OF CARDIO PROTECTIVE EFFECT OF AVERRHOA CARAMBOLA FRUIT IN ISOPROTERENOL INDUCED MYOCARDIAL INFARCTION IN RATS
Myocardial infarction develops due to ischemia induced oxidative stress. Averrhoa Carambola fruit (ACF) is reported to possess strong antioxidant activity which may protect the heart against isoproterenol (ISO) induced myocardial infarction (MI) in rats. To evaluate this activity Averrhoa carambolafruit extract (ACFE) (200mg/kg & 400mg/kg, p.o) was administered to male wistar rats (180-250g) f...
TIME-DEPENDENT EFFECTS OF TEMPERATURE AND HUMIDITY ON QUANTITY OF DNA IN SAMPLES OF HUMAN SALIVA, BLOOD AND SEMEN IN KUWAIT
Forensic science is growing rapidly in the world today. During the past ten years, medico-legal investigations highly expanded to include all areas of forensic science. The present aim of project investigated 29 samples of blood and 29 samples of saliva swabs that were collected from human volunteers. The saliva samples were collected by buccal swab but the blood samples were collected by Bode Sec...
PHYTOCHEMICAL PROFILING, ANTIBACTERIAL SCREENING AND ANTIOXIDANT PROPERTIES OF THE SACRED TREE (SHOREA ROBUSTA GAERTN.) OF JHARKHAND
Shorea robusta Gaertn., commonly called as Sal tree, is revered as a sacred tree by most of the tribals of Jharkhand, India. They do not worship it rather believe it to the abode of tribal goddess. The plant parts are used as a remedy against various ailments in combination with other ingredients. Preliminary phytochemical screening of the whole plant exhibited the presence of high concentration o...
PREVALENCE OF FOOD INSECURITY AND ITS ASSOCIATION WITH MUSCLE MASS, HAND GRIP STRENGTH AND GAIT SPEED AMONG ELDERLY IN TEHRAN
Background: Human muscle mass undergoes constant changes as one getting older. This change can be accelerated by inadequate nutrition. The aim of the study is to investigate the association between food insecurity and its association with muscle mass strength. Methods: The cross-sectional and case-control study was conducted among 310 men and 334 women (60 and over) from August 2014−July 2015 in...
SIMULTANEOUS ESTIMATION OF ALFUZOSIN AND DUTASTERIDE IN TABLET DOSAGE FORM BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
A simple, accurate, economic and rapid method for the simultaneous estimation of Alfuzosin and Dutasteride by RP-HPLC, as a result simple, economic, precise and accurate methods was developed and validated by Reverse Phase High performance Liquid Chromatography in tablet dosage form. The mobile phase consisted of Acetonitrile and Buffer solution in the ratio of (75:25) was eluted through universil...
SIMULTANEOUS STABILITY-INDICATING METHOD FOR THE DETERMINATION OF ABACAVIR, DOLUTEGRAVIR AND LAMIVUDINE BY RP-HPLC
A simultaneous stability-indicating reversed-phase high performance liquid chromatography (HPLC) method for analysis of abacavir (ABC), dolutegravir (DTG) and lamivudine (3TC) as the bulk drug and in the formulation was developed. Compounds were separated on Kinetex 5 µ C18 100 A (250 mm x 4.6 mm). A gradient program of mobile phase at different proportions of acetonitrile (ACN) and water was use...
ANTIBACTERIAL ACTIVITY OF MENTHA PIPERITA AND CITRUS LIMETTA AGAINST PROPIONIBACTERIUM ACNES (ANAEROBIC BACTERIA)
Acne vulgaris, the inflammatory disease of sebaceous follicles of skin with Propioni bacterium acnes (an anaerobic pathogen) play deliberate role in its pathogenesis by inducing certain inflammatory mediators. The aim of present study was to evaluate the antibacterial activity of crude ethanolic extract of Mentha piperata and essential oil of the peel of Citrus limetta (extracted by hydrodistillat...
Objective: Conventionally, sustained release pellets have been prepared in the form of reservoir systems by coating non-pareil pellets which is an expensive and tedious process. The aim of present study was to develop sustained release matrix (SRM) pellets of Nateglinide (NT), an oral antidiabetic agent, commonly used in the treatment of Type 2 Diabetes mellitus. Methods: The SRM pellet formulatio...
The best QSAR model were generated with left of adept and significant descriptors like electronic, lipophilic and topological, using multiple linear regression (MLR) and partial least square (PLS), model further explained by using forward feed neural network analysis (FFNN). QSAR is a kind of technique that directly correlates in between chemical structure to their biological activity. The best ML...
The crude methanolic study of Vanda roxburghii root was investigated for antioxidant, analgesic and cytotoxic activity. The extract of Vanda roxburghii root showed antioxidant activity in DPPH radical scavenging activity, nitric oxide scavenging power assay. In DPPH and NO scavenging assay the extract shown adequate antioxidant activity and the IC50 values in DPPH radical scavenging and NO scaveng...
Antimicrobial peptides (AMP) showed a broad spectrum of cytotoxic activity against cancer cells, which could provide a new class of anticancer drugs. In this study, we have evaluated the cytotoxic and apoptotic activity of magainin II, an AMP compared with 5-Fluorouracil (5-FU) in colo320 DM cancer cells, and magainin II cytotoxicity was assessed on vero cell line by MTT. The cells cultured in the...
ROLE OF MICROWAVE ASSISTED EXTRACTION FOR ISOLATION OF SAPONINS FROM SAPINDUS MUKORROSAI AND SYNTHESIS OF ITS STABLE BIOFUNCTIONALIZED SILVER NANOPARTICLES AND ITS HYPOLIPIDAEMIC ACTIVITY
This research emphasis on microwave assisted extraction of fruits of Sapindus mukorrosai. Aqueous fruit extract is subjected for the isolation of saponins, focusing only on higher molecular weight of saponins using dialysis membrane. On treatment of aqueous solution of silver nitrate with isolated saponin, stable silver nanoparticles were rapidly formed. The green synthesized silver nanoparticles ...
The present study describes the biochemical, antioxidant and anticancer properties of the crude and melanin free ink of Sepia prabahari which is rich in both organic and inorganic components. The quantitative analysis define the presence of protein (1.16 – 1.49 mg/ml), lipid (0.25 – 1.42 mg/ml), carbohydrates (0.01 – 1.14 mg/ml), ash (0.4 & 0.1%) and moisture (93.33%) content. The native...
A RAPID GAS CHROMATOGRAPHY- MASS SPECTROSCOPY METHOD FOR SIMULTANEOUS QUANTIFICATION OF ORNIDAZOLE AND MICONAZOLEFROM CREAM FORMULATIONS: DEVELOPMENT, VALIDATION AND APPLICATION
Combination drug formulations are better in terms of effectiveness and hence are used many times for treatment of diseases. The physico-chemical properties of the different API used in a formulation have a significant impact in the development of a single method for the analysis of such drugs. In the current research a rapid analytical method employing GC with Mass Spectroscopy detection has been ...
Medicinal plants are of important therapeutic aid for various treatments. Leucas cephalotes Spreng. up in cultivated fields as a weed, especially after a period of rain. In the present study the dried plant stems powder of Leucas cephalotes Spreng. was taken and subjected to extraction with ethanol and aqueous extraction was done on the marc left after ethanolic extraction. The antimicrobial activ...
STUDIES ON IN VITRO EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF STERCULIA FOETIDA L. BARK
The objective of the present investigation was to evaluate the antibacterial and antioxidant potential of n-hexane and methanolic extracts of Sterculia foeida L. bark. The antibacterial potential of Sterculia foetida L. bark was tested against human pathogens causing diarrhoea and dysentery such as Shigella flexneri (MTCC-9543), Salmonella enterica ser typhi (MTCC-733), Bacillus subtilis (MTCC-130...
A MODIFIED LIQUID CHROMATOGRAPHIC METHOD DEVELPOMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF BISOPROLOL FUMARATE AND HYDROCHLOROTHIAZIDE IN BULK AND TABLET DOSAGE FORM
A simple, precise, accurate, reproducible and economical reverse phase liquid chromatography method was developed and validated for the quantitative simultaneous estimation of Bisoprolol Fumarate and Hydrochlorothiazide in bulk and marketed formulations. Estimation of drugs in this combination was done with a C18 column Kromasil 100-5C18 column [250mm x 4.6mm].using mobile phase of composition A...
HAEMATOLOGICAL AND BIOCHEMICAL CHANGES IN PRE AND POST TREATMENT OF BUCCAL MUCOSA CARCINOMA PATIENTS
Oral squamous cell carcinoma is one of the leading causes of mortality in India. The various haematological and biochemical changes including liver enzymes imbalance occur in cancer treatment. The careful monitoring of serum biochemical and haematological changes had very important role in prognosis of disease. Therefore, the present study aimed to study haematological and biochemical changes ...
Protein kinases are key players in cellular signal transduction and perturbations in its activity leads to cancer. Targeting these seems to be sufficient to provoke apoptotic response, thus limiting the process of tumorogenesis. Among kinase inhibitors, imatinib has demonstrated magnificent clinical efficacy. But patients with advanced disease harbor faces resistance. Currently there is requisite ...
HPTLC METHOD DEVELOPMENT AND VALIDATION FOR STANDARDIZATION OF AYURVEDIC FORMULATION: MAHASHANKH VATI
Ayurveda is the primeval complete serving system in medical field. However, one of the barriers in the acceptance of the Ayurvedic formulation is the paucity of standard quality control outline. World health organization (WHO) in 1999 has given a detail procedure for the standardization of herbal drugs comprising of a single content but not for standardization of polyherbal formulations. Mahashank...
EVALUATION OF TOTAL PHENOLICS, FLAVONOID CONTENTS AND IN-VITRO ANTIOXIDANT PROPERTIES OF CATHARANTHUS PUSILLUS (APOCYNACEAE)
Objective: Present study was aimed to evaluate the total phenolic, flavonoid content and in vitro antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts of whole plant of Catharanthus pusillus. Methods: Antioxidant activity have been tested using various antioxidant model systems viz., DPPH, hydroxyl, superoxide, ABTS and reducing power. Results: Methanol ex...
QUANTIFICATION OF LINEZOLID AND CEFUROXIME AXETIL BY CHEMOMETRICS ASSISTED AND RP-HPLC METHODS: DEVELOPMENT AND VALIDATION
This paper describes simple, sensitive, accurate, rapid, precise and economical Chemometrics assisted and RP-HPLC methods for simultaneous estimation of Linezolid(LNZ) and Cefuroxime axetil(CEF) in bulk and tablet. Chemometrics offers a useful approach for an estimation of combined dosage form. The measurement carried out at wavelength 240 to 290nm with interval of 4nm for Linezolid and Cefuroxime...
Colorants are mainly used to impart a distinctive appearance to the pharmaceutical dosage forms. The present study was carried out to develop aluminium lake color of a dye obtained from black carrot using different adsorbents (Aluminium oxide or aluminium hydroxide) in different ratio by simply mixing them to get dye adsorbed onto the surface of adsorbent. Adsorption experiment was carried out for...
EMERGENCY OBSTETRICS TRAINING IMPROVING SKILLS AMONG HEALTH CARE WORKERS IN MIGORI AND NYERI COUNTIES, KENYA
Introduction: Utilization of skilled health care is effective in reduction of maternal and newborn morbidity and mortality. Sufficiently trained health workers can competently handle and manage obstetric complications. Health workers, despite having undergone a formal training, require regular refreshers to keep up to date with new, evidence-based information. Methods: This is an evaluation repo...
Biofilm formation is prevailing in almost all bacteria. They are responsible to cause infections like Peritonitis, Cystic fibrosis, Endocarditis, Otitis media, UTIs. The infections caused by the biofilms forming bacteria that colonize the indwelling medical devices are a major source of patient morbidity and implant failure. The study on biofilm formation on indwelling medical devices is importa...
SENSITIVITY AND RESISTANCE PATTERN OF ANTIMICROBIAL AGENTS USED IN CASES OF NEONATAL SEPSIS AT A TERTIARY CARE CENTRE IN WESTERN INDIA
Purpose: Neonatal sepsis, a major killer among neonate, necessitates urgent implementation of empirical therapy and later specific antimicrobial therapy against causative microorganism. Empirical therapy relies upon data obtained from previous studies. We conducted this study to evaluate the blood culture, sensitivity and resistance pattern of various antimicrobial agents in neonatal sepsis at Neo...
The aim of the study was to determine the anticonvulsant activity of methanolic extract of Lawsonia inermis leaves in albino rats. The anticonvulsant activity of methanolic extract of leaves of Lawsonia inermis (200 mg/kg and 400 mg/kg) was assessed in rats using maximum electroshock seizure (MES) test and pentylenetetrazole (PTZ) induced seizure test. The methanolic extract of Lawsonia inermis le...
STUDY OF DRUG PRESCRIBING PATTERNS IN PSORIASIS AND ASSESSMENT OF RATIONALITY USING SIGN AND NICE GUIDELINES AT A TERTIARY CARE TEACHING HOSPITAL
Introduction: Psoriasis is a chronic inflammatory disease of high prevalence. In India the prevalence of psoriasis varies from 0.44 to 2.8%. The main aim of drug utilization research is to facilitate the rational drug use. Without a precise knowledge of how drugs are being prescribed and used, it is difficult to suggest measures to improve the prescribing habits. The economic burden of psoriasis i...
Asenapine in Tablet dosage form is used as antipsychotic agent. Literature revealed that there is no single method for the simultaneous estimation of this drug in tablet dosage form, which prompted us to develop a simple, rapid, accurate, economical and sensitive UV spectrophotometric and RP-HPLC methods. The simultaneous estimation of UV method is based on the principle of additivity of absorbanc...
PREVALENCE OF DIAGNOSED AND UNDIAGNOSED HYPERTENSION IN A RURAL COMMUNITY: A HOME BASED SCREENING STUDY
Background: Hypertension is one of the global risks for mortality and is seen with drastic rise in developing nations with rise in age. Methods: This cross sectional study was conducted in Ottapalam town, Palakkad district, Kerala, India. Based on inclusion criteria (non-pregnant, age group between 35-75 years) 142 subjects were studied. Patient data (demographics, lifestyle factors, medical repor...
Urolithiasis is a relevant clinical problem in every day practice with a subsequent burden for the health system. Animal models of disease most closely replicate the sum of physiological conditions present in humans. Rats and mice are however, the two most commonly used experimental animals in medical research including stone disease. The oxalate metabolism in the rat is nearly identical to that i...
COST-EFFECTIVENESS AND PHARMACOECONOMIC ANALYSIS OF TWO DIFFERENT COMBINATIONS OF DRUGS USED IN OSTEOPOROSIS PATIENTS IN A TEACHING HOSPITAL
The purpose of the study is to update and review the latest developments related to modelling and economic evaluation of osteoporosis and further to present a reference model for the assessment of the cost of the prevention and treatment of osteoporosis. To find out the most cost-effective drug combination between the two combinations (Alendronate + Vitamin D supplements & Strontium ranelate +...
EVALUATION OF ADVERSE EFFECTS DUE TO ANTIDIABETIC AND CARDIOVASCULAR DRUGS IN A TERTIARY CARE HOSPITAL IN KOLKATA, WEST BENGAL, INDIA
Purpose: Diabetes mellitus is a significant burden to the present society, associated with various co-morbid conditions like hypertension, cardiovascular diseases, etc. A number of drugs are available to handle these health problems successfully. However, therapeutic success of such diseases is limited due to adverse effects of drugs or medicines. The aim of the present study was to evaluate the...
Chronic Kidney Disease is a considerable problem requiring coincident use of several medications in order to manage the condition and co morbidities. Medication adherence rate are diminished by complex drug regimens. Generally poly pharmacy fabricates chances of poor adherence and adverse drug reactions (ADRs). In Chronic Kidney Disease (CKD) functioning of kidney is poor and decreased elimination...
PREVALENCE OF -56C/T REGULATORY POLYMORPHISM IN INTERFERON-γ RECEPTOR 1 PROMOTER REGION KNOWN TO BE POTENTIAL RISK FACTOR FOR HEPATITIS B VIRUS INFECTION IN NORTH INDIAN HEALTHY POPULATION
Interferon-γ (IFN-γ), a defense molecule, mediates its response against viral infections like Hepatitis B virus by binding to interferon gamma receptor 1 (IFNGR1) on cytotoxic T lymphocytes (CTLs). Hence, interferon gamma receptor 1 (IFNGR1) gene expression is vital in the eradication of the HBV virus. It has been demonstrated by some studies that -56C/T SNPs in IFNGR1 gene promoter region can b...
FORMULATION AND IN-VITRO CHARACTERISATION OF SOLID – SELF NANOEMULSIFYING DRUG DELIVERY SYSTEM (s-SNEDDS) OF RILPIVIRINE
Rilpivirine (RILP) is non-nucleoside reverse transcriptase inhibitor. It is indicated for the treatment of HIV-1 infection. The current investigation was aimed to formulation and evaluation of solid self nano emulsifying drug delivery system (S-SNEDDS) for Rilpivirine to enhance solubility and dissolution rate. Solubility of drug was determined in different vehicles. Pseudo ternary phase diagram g...
The paper focuses on different marketing techniques in Pharmaceutical Industry with reference to anti-ulcer drugs. This is an industry where a number of characteristics like, efficacy, quality and safety matters while delivering a product (i.e. the drug). This study comes up with drug development and the various aspects of bringing the product to the market. Secondly, it deals with determining the...
Due to the increasingly tight controls around compliance, pharmaceutical firms continue to seek more efficient ways to track, document and report on code changes in regulated applications such as those developed for managing samples and inventory. Such applications and electronic signatures associated with application development and workflows are subject to stringent regulations such as Sarbanes-...
BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF CANDESARTAN BY DERIVATIVE SPECTROSCOPY (FIRST ORDER)
Candesartan cilexetil, a prodrug is a racemic mixture containing one chiral center at the cyclohexyloxy-carbonyloxy- ethyl ester group. It is soluble in dimethyl formamide, acetone, methanol, 0.1 N sodium hydroxide solution and insoluble in water. Objective of the present study was to develop a simple, sensitive, accurate, precise and rapid derivative spectrophotometric bioanalytical method for th...
PREPARATION, CHARACTERIZATION AND IN-VIVO RELEASE OF NANOMEDICINE (SOLID LIPID NANOPARTICLE) ASSOCIATE WITH THE EXTRACT OF PTEROSPERMUM ACERIFOLIUM FOR THE SCREENING OF NEUROBEHAVIOURAL
Advances in nanoparticulate frameworks for enhanced medication conveyance show an extraordinary potential for the organization of urgent dynamic particles. The fundamental motivation behind creating elective medication conveyance advances is to build proficiency of medication conveyance. Pterospermum acerifolium is normal plant in India is viewed as carminative, stimulant and emmenagogue. The poin...