Volume 10 (2019) - Issue 12, December
Antibiotic is the powerful invention of science to damage bacterial infections. But nowadays, the use of careless increasing doses of this antibiotic has led to increasing the resistant power of enemy bacteria. Bacteria are caused by many infectious diseases by proliferation within quorum sensing (QS) mediated biofilms. Quorum sensing can develop microbial pathogenic traits, including biofilm form...
Schizophrenia is a chronic and severe mental disorder that affects how a person thinks, feels, and behaves. People with schizophrenia may seem like they have lost touch with reality. Schizophrenia is a split personality disorder which is related to mental illness. Generally, schizophrenia affects approximately 1 percent of all adults’ population. Schizophrenia is different types such as parano...
The skin is the largest organ of the human body and easily accessible. It’s potential as an alternative route for administering drugs for both systemic and local effects has attracted considerable interest. Most of the topical medications which are applied on the skin or mucous membrane to treat ailments include a wide range of creams, foams, gels, lotions, ointments, eye drops, ear drops, etc. ...
DOXORUBICIN AND CHLOROQUINE: A COMBINATION THERAPY TO OVERCOME THE MULTI DRUG RESISTANCE IN CANCER – A REVIEW
Cancer is a genetic disorder that can happen to anybody; anytime. The treatment, where one or more chemotherapeutic agents (anti-cancer drugs) are used either to treat the cancer patients (curative intent with one or combination of anti-cancer drugs) or to prolong the life and to reduce the symptoms in cancer patients is known as Chemotherapy. But nowadays it is observed that when these chemothera...
Recent past has witnessed the promising roles of natural gums as pharmaceutical ingredients. Gums became important excipients in many pharmaceutical preparations owing to their abundance, biodegradability, non-toxicity and comparatively low cost. They have notable applications in pharmaceutical preparation as binders, disintegrants, suspending agents, emulsifiers. The use of these gums as gelling ...
Inflammation is the vascular event shown by the body against the external material by activating different cells and releasing various chemical mediators to prevent noxious effects. Many drugs like steroidal and non-steroidal drugs are available till the date to treat inflammation but have a number of side effects and centralized on to counteract the COX and LOX pathways. This perspective will use...
HYPERHOMOCYSTEINEMIA: A SILENT HASSLE FOR HUMAN BEINGS, ALLIED COMPLICATIONS AND PRAGMATIC STRATEGIES
Hyperhomocysteinemia is a metabolic catastrophe characterized by an elevated level of homocysteine above 15 mmol/L in blood. It is an established risk factor for cardiovascular anomalies. The illness has also crucial impact on other organs and tissues of body. Its morbid effects have been seen during many chronic conditions. It is also considered as a metabolic crunch during gestation as well as g...
Osteomyelitis is a very serious disease caused by bacteria, especially Staphylococcus aureus, which directly attacks the bones tissues. Many physicians and surgeons are still looking for the basic cure of the disease. This article mainly focuses on the different kinds of treatments using new drug formulations. Vancomycin coated with chitosan polymers, biodegradable scaffolds, use of major limb amp...
This paper is to review the green catalytic synthesis of organic reactions and discussed its advantages over the conventional method of synthesis. Recent advanced technologies have key trend to reduce the negative impacts of chemical environment and helps to obtain sustainability in chemical production. This review tries to bring many catalytic techniques that are safer in nature and more economic...
Introduction: Juvenile idiopathic arthritis (JIA) is chronic inflammatory arthritis in children and adolescents. The symptoms present in JIA are more comparable with features of Aamavata Vyadhi mentioned in Ayurvedic classics. JIA can be treated by Shodhana and Shaman therapy of Ayurveda which is free from any side effects. The main aim of this article is to elaborate on JIA and its treatment by d...
DESIGN, SYNTHESIS, AND EVALUATION OF 1,4-NAPHTHOQUINONE DERIVATIVES AS POTENTIAL EPITHELIAL GROWTH FACTOR RECEPTOR INHIBITORS
In this manuscript, we designed and synthesized 1,4-naphthoquinone derivatives. These derivatives were characterized using the different analytical techniques such as 1H NMR, 13C NMR, FTIR, Mass spectroscopy, melting point, and elemental analysis. The synthesized compounds (MB (1-10)) further subjected to assess the anticancer activity using different cancer cell lines such as MCF-7, HeLa, and Hep...
PHYTOCHEMICAL ASSESSMENT AND METABOLIC FINGERPRINTING OF BOERHAVIA DIFFUSA AND CEPHALANDRA INDICA FROM INDIAN SUB-CONTINENT
The medicinal plants, Boerhavia diffusa and Cephalandra indica are both perennial climbing herbs commonly used in traditional system of medicine for the treatment of several ailments because of their healing properties. The present study aimed to identify the phytochemical constituents of methanolic extracts of these plants, followed by their metabolic fingerprinting, which was carried out by HPTL...
Objective: The present study was designed for Isolation of phytoconstituents from pharmacologically potent extracts of leaves of Cassia uniflora based on in-vitro pharmacological screening and their subsequent characterization. Methods: Crude extracts of leaves, stems, and fruits of cassia uniflora were prepared using various solvents such as water, methanol and hydro alcohol. These extracts were ...
PREPARATION, CHARACTERIZATION AND EVALUATION OF ANTIFUNGAL ACTIVITY OF CLOTRIMAZOLE NANOSTRUCTURED LIPID CARRIERS
Objectives: The aim of this work was the preparation of Clotrimazole (CM) loaded nanostructured lipid carriers (CM-NLC) and the examination of in-vitro antifungal activity of prepared formula. Methods: High shear hot homogenization method was used for CM-NLC preparation. Different ratios of Compritol AT 888 and Labrasol were used and combined with two types of surfactants: Kolliphor P188 or Kollip...
Objective: The objective of the study was to estimate the effects of Non-caloric Artificial Sweeteners (NAS) on glycaemic status in male Swiss albino mice. Methods: 36 male Swiss albino mice were selected and divided into six groups of 6 animals each. Group 1 served as control and received a normal pellet diet with drinking water. Groups 2, 3, 4, 5 and 6 received normal pellet diet and drinking wa...
PREPARATION AND CHARACTERIZATION OF HERBAL NANOFORMULATION CONTAINING ANDROGRAPHIS PANICULATA EXTRACT
This study aimed to investigate the preparation and characterization of polymeric nanoparticles containing extract of Andrographis paniculata using Eudragit S 100 polymer. The methanolic extract of aerial parts of Andrographis paniculata Nees (Acanthaceae) was prepared and standardized for andrographolide content using the High-Performance Liquid chromatography technique. Three formulations contai...
Delonix elata (D. elata) whole plant was chosen to evaluate in-vitro anti-oxidant activities. The total polyphenols and flavonoids were determined after the extraction. DPPH (2, 2'-diphenyl-1-picrylhydrazy), H2O2 (Hydrogen peroxide), NO (Nitric oxide) and TBARS (Thiobarbituric acid) methods were used to evaluate antioxidant activities of D. elata whole plant. The results showed that the highest le...
QSRT STUDIES OF 2-(5-ARYL-1, 3, 4-OXADIAZOL-2-YL)-N-(5-METHYLISOXAZOL-3-YL)-ACETAMIDE DERIVATIVES AS AN ANTIMICROBIAL AGENTS
Quantitative structure relationship techniques (QSRT) is among the most widely used computational technology for analog-based drug design. A molecular modeling approach using theoretical, computational analysis through chem sketch ACD lab online software was done for determination of probable antimicrobial activity. To develop a pharmacophoric model for inhibition, QSRT parameters PCP, ADME, and t...
A NEW TARGET FOR DIABETES: HOMOLOGY MODELING AND IDENTIFICATION OF POTENTIAL LEADS THROUGH VIRTUAL SCREENING
Monocarboxylate transporter 11 (MCT11) is a transporter protein encoded by the SLC16A11 gene. MCT11 protein is found to play an important role in many biological activities in different species. MCT11 helps in hepatic lipid metabolism by catalyzing the transport of pyruvate molecules across the membrane. It is also found to be involved in regulation of Diabetes. Therefore, MCT11 protein is taken i...
ALTERATIONS IN LIPID PROFILE PARAMETERS OF ALBINO RAT AFTER INTOXICATION OF MERCURY SELENIDE NANOPARTICLES
Nanoparticles are of great scientific interest, in fact, a bridge between bulk materials and atomic or molecular structures. The main characteristic of nanoparticles is their extreme smaller size, which falls in the transitional zone between molecules and the corresponding bulk materials. Lipids and fatty acids are a family of molecules classified within the lipid macronutrient class. The present ...
PRECLUSION OF HYPOXIA: IDENTIFICATION OF POTENTIAL INHIBITOR AGAINST HIF-1 ALPHA PROTEIN THROUGH MOLECULAR DOCKING
Low level of oxygen in tissues leads to hypoxia, and hypoxia-inducible factors (HIF) regulate the hypoxic and normoxic conditions in various tissues. During normoxia, HIF-1α binds to von Hippel-Lindau E3 ubiquitin ligase complex that targets HIF-1α to the ubiquitin-proteasome pathway for proteolytic destruction. But in hypoxia, HIF-1α move to the nucleus where it binds to CBP/p300 at CH1 domain...
In recent years, there is an increasing interest in finding antioxidant phytochemicals for the prevention and treatment of complex diseases like diabetes, cancer, and atherosclerosis, which are associated with excessive biological oxidative stress. Plants are rich source of natural antioxidants that inhibit the propagation of free radical reactions and protect the human body from degenerative dise...
HPLC ANALYSIS OF FLAVONOIDS FROM PROPOLIS OF DIFFERENT HONEYBEE SPECIES IN SELECTED LOCATIONS OF BANGALORE
Propolis is a natural resinous substance with a wide range of biological properties, synthesized by bees from bud exudates of some plants. Bees make use of propolis as a building material and to defend the hive. The chemical composition of propolis varies depending on vegetation and species having plethora of therapeutic activities. To detect compounds present in propolis an efficient, precise, an...
The aim of present work is to design a Tenoxicam loaded nanogel for the anti-inflammatory and analgesic effect of prepared nanogel. Tenoxicam is one of the NSAIDs of oxicam class; it is very potent and useful for chronic use in rheumatoid arthritis and osteriostatis. The formulations of nanogel based materials have high drug loading capacity, biocompatibility, and biodegradability which are the ke...
Objectives: The main objective was to find out a suitable and effective natural release retardant, which can be used in matrix tablet preparation along with synthetic excipients. Methods: Tamarind seed polysaccharides (TSP) were isolated from Tamarindus indica by hot extraction method followed by freeze-drying and yield was found to be 16.85%. Microbial studies confirmed that TSP doesn’t support...
SIMULTANEOUS ESTIMATION OF AMANTADINE HYDROCHLORIDE AND OSELTAMIVIR PHOSPHATE USING PRECOLUMN DERIVATIZATION TECHNIQUE
A simple, efficient HPLC method has been developed and validated for simultaneous determination of Amantadine hydrochloride and Oseltamivir phosphate using pre-column derivatization with phenyl isothiocyanate (PITC). The chromatography separation was achieved on Phenomenex C18 column of 250 × 4.6 mm 5 µm particle size, mobile phase used for chromatographic run consisted of acetonitrile and water...
In the present research, newly developed hydrotropic solid dispersion technique utilizing the use of aqueous solvent was developed, to avoid the utilization of organic solvent and all together declining their toxicity. This technology was employ intended for preparing the dispersion of Eluxadoline by using new hydrotropic agent sodium caprylate with using different ratio. Prepared formulations wer...
FORMULATION AND EVALUATION OF ANTIDIABETIC CAPSULES OF KAEMPFEROL-3-O-Β-D-6”-(P-COUMAROYL) GLUCOPYRANOSIDE EXTRACTED FROM ALLIUM CEPA L.
Allium cepa L. has known potent antidiabetic effects due to several of its constituents, including kaempferol -3-O-β-D-6''-(p-coumaroyl) glucopyranoside (kaempferol). This preliminary work was aimed at preparing oral hard gelatin capsules of kaempferol obtained from Allium cepa L. In order to obtain more potent hypoglycemic activities, lower side effects with good patient compliance over the crud...
ESTIMATION OF SOLID DOSAGE FORMS OF COMBINED ANTIHYPERLIPIDAEMIC DRUGS BY VALIDATED REVERSE PHASE LIQUID CHROMATOGRAPHIC METHOD
A reverse-phase high-performance liquid chromate-graphic method (RP-HPLC) was developed for the simultaneous estimation of combined dosage form of ezetimibe with simvastatin and rosuvastatin using RP-C18 column. For estimation of ezetimibe and simvastatin, the mobile phase (acetonitrile: water and pH adjusted to 4.5 with acetic acid) was pumped at a flow rate of 0.8 ml/min in the ratio of 85:15% v...
FABRICATION OF TWEEN 80 LIGAND COATED CHOLESTEROL-SOYA LECITHIN BILAYERED NANOLIPOSOMES FOR IMPROVED RELEASE OF MEROPENEM AGAINST ARTIFICIAL BLOOD BRAIN BARRIER
The blood-brain barrier is the most challenging physiological obstacle for drug moieties for reaching the central nervous system. Meropenem is a drug used against meningitis, however crossing BBB is also a challenging realm of problem for the same. Therefore attempt has been undertaken to strategically design Meropenem loaded cholesterol-soya lecithin nanoliposomes (MCSLN). MCSLN was prepared by n...
APPLICATION OF STABILITY INDICATING UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF CAFFEINE IN GEL FORMULATION, PLASMA AND URINE
A simple, sensitive and stability indicating UV spectrophotometric method was developed and validated for the estimation of caffeine in a gel formulation, plasma and urine samples using acidified alcohol as solvent. At absorbance maxima of 265 nm, the linearity was found to be in range of 4-20 µg/ml with correlation coefficient 0.9988. The method was applied for estimation of caffeine in a gel fo...
EVALUATION OF NOVEL LIGAND FOR THE MAINTENANCE OF NATIVE Aβ(1-42) PEPTIDE CONFORMATION: RELEVANCE TO ALZHEIMER’S DISEASE
Alzheimer's disease (AD) is a disorder of the central nervous system with progressive neurodegeneration, cognition, and memory loss. A major molecular hallmark of the disorder includes extracellular deposition of the Amyloid beta-peptide (Aβ) in senile plaques, the appearance of intracellular neurofibrillary tangles. The Aβ peptide is produced by sequential cleavage of the Amyloid precursor prot...
OPTIMIZATION OF PLURONIC LECITHIN ORGANOGEL OF TERBINAFINE HYDROCHLORIDE USING DESIGN OF EXPERIMENTS FOR THE TREATMENT OF FUNGAL DISEASES
The research work focused on the optimization of Terbinafine HCl (TBH) pluronic lecithin organogels (PLOs) for increasing the penetration and efficacy of the drug. The factors affecting the PLOs such as viscosity and percentage drug release, were screened and optimized using the Box-Behnken design. The optimum formulation based on desirability (0.995) exhibited 3139.92 Cp for viscosity and 93.4561...
2-Pyrazolines are well known, and important nitrogen-containing five-membered heterocyclic compounds which belong to the family of azoles and have been found to possess considerable activities, like anticancer, antibacterial, antifungal, immunosuppressant and antiamoebic activity. In the present work, ten novel pyrazolines were prepared from chalcones, characterized and evaluated for their antibac...
Objective: Physically modified forms of BCS class II drug Nabumetone (NBT) was studied for dissolution in water and pharmacopoeial dissolution media (2%w/v sodium lauryl sulfate in water). Characterization was to identify the suitability of physical processing like heating, cooling, grinding, and milling operations anticipated during commercial manufacturing of dosage forms. Materials and Methods:...
DESIGN, SYNTHESIS, DOCKING STUDIES AND BIOLOGICAL EVALUATION OF NOVEL HYDROXAMIC AND CARBOXYLIC ACID DERIVATIVES AS HISTONE DEACETYLASE ENZYME INHIBITORS
A new series of hydroxamic (5a-5j) and carboxylic acid analogs (4a-4j) based on the 1, 3, 4-thiadiazole scaffold was designed and synthesized to explore its potential as new antitumor agents. The chemical structures of the compounds were confirmed based on TLC, IR, 1H NMR, and 13C NMR analysis. Molecular docking studies showed that the target compounds correctly dock into the binding pocket of his...
DFT INVESTIGATION OF GEOMETRICAL STRUCTURE, IR AND RAMAN SPECTRA OF VINYL HALIDES CH2=CH–X (X IS F, Cl AND Br)
The geometrical structure and conformational energy stability of vinyl halides CH2=CH–X (X = F, Cl, and Br) were examined by using Density Functional Theory calculations (B3LYP method in combination with 6-311G* basis sets). The values of HOMO-LUMO gap (DE) for vinyl halides (fluoride, chloride and bromide) were 7.68 (eV), 7.10 (eV) and 6.55 (eV) respectively. The calculated geometrical paramete...
STUDY OF MANGIFERIN ISOLATED FROM LEAVES OF MANGIFERA INDICA ON MYELOID LEUKEMIA ALONG WITH DIFFERENT HUMAN CANCER CELL LINES
In different myeloid leukemia cases resistance to diverse treatment regimen owes to multiple molecular mechanisms of cellular network. Epigenetic dysregulation, gene mutations, overexpression of multidrug resistance genes, abnormal immune function, the presence of chemotherapy-resistant leukemia-initiating cells, and aberrant signaling pathways could be the lead cause of this kind of aberration. S...
STUDY OF EFFECT OF METFORMIN ON LIPID PROFILE IN TYPE 2 DIABETES MELLITUS IN A TERTIARY CARE TEACHING HOSPITAL
Type 2 diabetes mellitus poses an important health care problem because of its associated two to threefold increased risk of cardiovascular disease and its high prevalence and incidence worldwide. Dyslipidemia is an independent risk factor for severe morbidity and mortality in terms of cardiovascular risk in type-2 DM patients. Studies have shown an association between metformin and lipid profile ...
ETHNOGYNAECOLOGICAL DISORDERS OF MEDICINAL PLANTS USED BY TRIBAL PEOPLE IN GEDAMALAI, NAMAKKAL DISTRICT, SOUTHERN EASTERN GHATS, TAMIL NADU
The present exploration is an attempt to an ethnobotanical study was conducted in Eastern Ghats of Tamil Nadu, for the investigation of a medicinal plant used to treat several diseases by the local health healers by Gedamalai Tribes, Namakkal District. Data were collected during March 2015-January 2016. Quantitative analysis, including the informant consensus factor and use-value was performed to ...
Objective: To explore the Teratogenicity of isolated Polyphenols of Symphyocladia latiuscula (PSL) on pregnant female rats. Experimental Design: The marine alga Symphyocladia latiuscula were the preparation of methanolic extract followed by isolation of Polyphenols. The Teratogenic study was performed according to the OECD guidelines, no 415. And the test drug; PSL 100, 200 and 1000 mg/kg and posi...
THE HEATH RELATED QUALITY OF LIFE (HRQOL) FOR HYPERTENSIVE PATIENTS IN EASTERN PROVINCE IN SAUDI ARABIA: CROSS SECTIONAL STUDY
Background: Hypertension is associated with the flow of blood under high pressures, and is a risk factor for coronary heart disease. Methodology: We designed a prospective observational study that included 280 participants to investigate the effect of hypertension on the health-related quality of life (HRQOL) for hypertensive patients in the eastern province of KSA. To measure HRQOL, this study us...
DEVELOPMENT AND EVALUATION OF CANDESARTAN CILEXETIL TABLETS USING CRYSTAL AGGREGATES PREPARED BY POLYMER ENRICHED BRIDGING LIQUID
The main objective of the study was to formulate tablets of Candesartan cilexetil using the crystal agglomerates for the enhancement in flow properties and solubility. The crystal agglomerates were prepared by polymer enriched bridging liquid (PEBL) technique. The process requires three solvent systems, the good solvent for the drug, the bad solvent and the bridging liquid. The hydrophilic polymer...
SCREENING FOR PHYTOCHEMICAL, FLAVONOID CONTENT, ANTIOXIDANT AND ANTHELMINTIC POTENTIAL OF SIDA SPINOSA
Background: Sida spinosa (Family: Malvaceae) is a well-known herb, has considerable medicinal significance in the tribal region of Chhattisgarh. Several ethnomedicinal survey reports have been revealed its potential to cure ailments such as skin diseases, diarrhea, gonorrhea, and asthma. Objective: The aim of the present study to screen phytochemical, flavonoid content, antioxidant and anthelminti...
CHARACTERIZATION OF LIGNINOLYTIC ENZYMES AND DECOLOURIZATION OF SELECTED TEXTILE DYES FROM THE BLUSHING BRACKET MUSHROOM, DAEDALEOPSIS CONFRAGOSA
The effect of a wide range of culture conditions on the production of laccase (Lac), Lignin peroxidase (Lip) and Manganese peroxidases (MnP) by Daedaleopsis confragosa was studied. A ligninolytic enzyme-producing bracket mushroom, Daedaleopsis confragosa was isolated from tree trunk from Karwar coastal region, Karnataka, India and subjected to production and dye decolorization study. Ligninolytic ...
The present study concerns the development, characterization, and optimization of nanoparticulate oral formulations of Tolbutamide using poly (ԑ-caprolactone) which is a biodegradable polymer. Emulsion Solvent Evaporation technique was used for preparation of drug-loaded polymeric nanoparticles. This research was carried out to improve the therapeutic efficacy of tolbutamide by increasing its bio...
AN INVESTIGATION OF THE DNA BINDING PROPERTIES OF Mn2+, Co2+ AND Ni2+ COMPLEXES WITH 2-AMINOBENZONITRILE AND OCTANOATE ION AS LIGANDS
Three novel metal complexes of 2-aminobenzonitrile (ABN) and octanoate ion (OC) with Mn2+, Co2+, and Ni2+ have been prepared by using microwave irradiation. The DNA-binding properties of the free ligand 2-aminobenzonitrile and its Mn2+, Co2+ and Ni2+ complexes have been investigated by fluorescence measurements. The results suggest that ABN, Mn2+, Co2+ and Ni2+ complexes both bind to DNA via an...
The liver is a vital organ of the human body. It is responsible for protecting the body from harmful substances and the maintenance of good health. In last few years, various types of liver disorders, such as drug-induced liver disease, hepatomegaly, fatty liver disease, liver cirrhosis, autoimmune hepatitis, chronic viral hepatitis, autoimmune hepatitis, etc. are become a people’s health proble...
ANGIOTENSIN CONVERTING ENZYME INHIBITION: A MODIFYING PLAYER ON ATHEROGENESIS IN HEMODIALYSIS PATIENTS WITH HEPATITIS C INFECTION
Background and Aim: This study aimed to investigate certain non-traditional risk factors for atherosclerosis in CKD hemodialysis patients with and without HCV infection. Methods: The eligible hemodialysis patients attending our renal dialysis unit were subjected to demographic assessment, testing for HCV infection, basic biochemical profile, and assaying for serum insulin levels, angiotensin conve...
ISOLATION, IDENTIFICATION AND QUANTIFICATION OF BIOACTIVE COMPOUNDS FROM AMARANTHUS HYPOCHONDRIACUS LEAF EXTRACT: A VITAL SOURCE OF POTENT NATURAL ANTIOXIDANTS
In this study we aimed at isolation, identification, and quantification of two phenolic compounds reported for the first time in leaves of Amaranthus hypochondriacus. A non targeted GC-MS metabolite profiling of hexane extracts of amaranth leaves was done which indicated the presence of major phenolic compounds. Two alkylated phenols namely 2,4-di-tert-butyl phenol (A) and 3-(3,5-di-tert-butyl-4-h...
Cancer is one of the emergings, and best-understood neoplasm’s from a genetic perspective, yet it remains the second most common cause of cancer-related death, indicating that some of its cancer cells are not removing completely by current therapies. Chitosan is a polysaccharide β-linked D-glucosamine and N-acetyl-D-glucosamine were used as conjugate to 5Fluorouracil. 5FU is one of the most com...
DEVELOPMENT AND VALIDATION OF UPLC METHOD FOR ESTIMATION OF % RELEASE OF SOME ANTI-HIV DRUGS IN FIXED DOSE COMBINATION
A simple, accurate and precise UPLC method was developed and validated for dissolution testing of Lamivudine, Tenofovir disoproxil fumarate and Efavirenz in tablet dosage form. The separation was achieved under optimized chromatographic condition on an Acquity UPLC BEH (50 mm × 2.1 mm, 1.7 μm) column with mobile phase consist of 20 mM potassium dihydrogen phosphate buffer pH 2.5: acetonitrile wi...
ESTIMATION, RECOVERY STUDY & ASSAY OF MARKETED DOSAGE FORM OF BUPROPION HYDROCHLORIDE THROUGH DERIVATIVE SPECTROSCOPIC METHOD
Depression is the most common and spreadable disorder in recent age as people are going through a lot of pressure in their daily work caliber and so many unconditional events. A new approach called derivatization spectroscopy was developed for the anti-depressant agent, marketed formulation of Bupropion hydrochloride, to confirm the interfering substances from the excipients of solid dosage form, ...
A NATURAL COUMESTAN WEDELOLACTONE TARGETS INFLAMMATORY CYTOKINES TO INITIATE APOPTOSIS IN HUMAN CERVICAL CANCER CELLS
Objective: Wedelolactone is a naturally (WDL) occurring coumestan of phytoestrogen category that possess anticancer and anti-inflammatory property. In this study, the anti-inflammatory effect of WDL on human cervical cancer cells (HeLa) and initiation of apoptosis mechanism were investigated. Methods: MTT assay and Trypan blue assay were demonstrated to detect the inhibitory effects of WDL on cell...
As a part of our ongoing effect to develop a biodegradable medicated film for wound healing using chitosan and HPMC as polymers. The drug used is Silver sulfadiazine. This formulation produces better biodegradability and bioadhesion. The biodegradable film is non-toxic and non-irritant to the skin. An animal wound model was performed on the back of the rats and treated, respectively with medicated...
GRADIENT HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR DETERMINATION OF RELATED SUBSTANCES IN [(3aS, 4R, 6aR)-2, 3, 3a, 4, 5, 6a-HEXAHYDROFURO [2, 3-b] FURAN-4-YL] N-[(2S, 3R)-4-[(4-AMINOPHENYL) SULFONYL-(2-METHYLPROPYL) AMINO]-3-HYDROXY-1-PHENYLBUTAN-2YL] CARBAMATE DOSAGE FORM
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for quantification of Darunavir in bulk and its tablet dosage form. The separation was carried out on Zorbax Eclipse XDB C18 (150 × 4.6 mm; 5 µm) column at 35 °C temperature using ammonium acetate buffer as mobile phase-A, acetonitrile (100%) as mobile phase-B. The flow rate was 1.0 ml/min and effluent ...
Introduction: In spite of the progress made in surgical techniques for cataract removal all over the world during last ten years; still age related cataract remains the leading cause of visual impairment. The etiology of cataract is multi-factorial and oxidative damage is one of the causes. Aim: To estimate oxidative stress by markers like enzymatic antioxidant Superoxide Dismutase (SOD) and lipid...
ANTIMICROBIAL SCREENING AND ANTI-ULCEROGENIC EFFECTS OF LEPIDIUM SATIVUM EXTRACT ON H. PYLORI INDUCED GASTRIC ULCER IN A RAT MODEL
Purpose: Helicobacter pylori (H-pylori) infection has been commonly associated with gastric ulcers. Lepidium sativum (L. Sativum) is an herbaceous edible plant, botanically related to mustard and watercress with remarkable pharmacological features. In this study, we investigate the anti-microbial and anti-oxidant effect of L. sativum seed methanolic extract in-vitro and correlate this with the gas...
SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF SOME NEW HETEROCYCLIC DERIVATIVES OF CHALCONE AS ANTIHYPERGLYCEMIC AGENTS
Protein tyrosine phosphatase 1B (PTP1B) is an important target for diabetes since inhibition of PTP1B offers therapeutic benefits in insulin-resistant diabetes. Considering this fact in present investigation various heterocyclic derivatives of chalcone were synthesized and evaluated for their PTP1B inhibitory activity as antihyperglycemic agents. Heterocyclic derivatives; isoxazoline (II A-II E), ...
DEVELOPMENT AND EVALUATION OF HP-β-CD COMPLEXATION BASED NOVEL OPHTHALMIC GEL FORMULATION OF NEPAFENAC
Nepafenac is an analgesic and anti-inflammatory drug, used in post-operative ocular pain and presently available in the market in the form of 0.1% ophthalmic suspension eye drop due to aqueous insolubility of the drug. The present formulation is not only poor patient compliant due to suspension form but also suffers from a short corneal residence and inadequate ocular bioavailability. The objectiv...
PHYTOCHEMICAL ANALYSIS AND DETERMINATION OF ANTIMICROBIAL, ANTIOXIDANT AND ANTICANCER ACTIVITY OF THE LEAF ETHANOLIC EXTRACTS OF PIPER SARMENTOSUM ROXB. IN LAPUYAN ZAMBOANGA DEL SUR, PHILIPPINES
Piper sarmentosum Roxb. belongs to the genus Piper in which traditional application described as remedy for several diseases. Considering its vast potential as effective pharmacological agent, a study was conducted to assess the phytochemical and biological activities of P. sarmentosum leaf extracts, with particular focus on their anti-microbial, anti-oxidant and anticancer properties. Results for...
PANCREATIC BETA CELL PROTECTIVE EFFECTS OF FLEMENGIA STROBILIFERA EXTRACTS IN COMBINATION MODEL OF HIGH FAT DIET AND STREPTOZOTOCIN INDUCED TYPE 2 DIABETES
The current study deals with anti-diabetic and pancreatic protective effects of Flemengia strobilifera extracts in a high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetes model. Toluene extracts were orally administered at the dose of 10, 30 and 100 mg/kg and metformin was used as a standard anti-diabetic drug at 10 mg/kg dose orally. Blood glucose, lipid profiles, pancreatic oxidat...