Volume 6 (2015) - Issue 8, August
Recently white mulberry has attracted a lot of interest as source of bioactive constituents used in phytopharmacy and in functional food formulation. In practice, active compounds are separated from plant material by extraction process using different solvents and extraction conditions. The most commonly solvents used for extraction are water, ethanol, methanol, acetone, ether and their mixtures. ...
Today, humans and wildlife are constantly exposed to thousands of chemical residues, through air, food and water. Organochlorines are widespread pollutants and comprise a variety of compounds containing carbon, hydrogen, and chlorine. Chlorinated insecticides, solvents, and fumigants are widely used around the world. Exposure to organochlorine chemicals has been associated with many deleterious ef...
In the battle against cancer, platinum drugs have contributed a lot so far but still the prerequisite is to mend their flaws e.g., resistance and reduced sensitivity. In addition to side effects, intrinsic and acquired resistance decreases the effects of cisplatin on cancer cells by lowering the formation of DNA adducts and subsequent DNA damage. Resistance against cisplatin includes; the increase...
The Human Papilloma Viruses (HPV) are non-enveloped, small DNA virus, which are responsible for worldwide problem. HPVS can be associated with different varieties of epidermal manifestations. It belongs to the Papovavirus family. On the basis of oncogenic potential these have been classified as high risk and low risk types of virus. Currently histopathological techniques are widely being used as t...
Penicillin antibiotics have been the main-stay armamentarium in the fight against bacterial diseases due to their availability and ease of use. Penicillin resistance has been attributed to modification of penicillin-binding proteins, production of β-lactamases, overexpression of efflux pumps and reduced permeability. In the past 10 years, research was centred on Gram-positive bacteria, particular...
DEVELOPMENT OF ORALLY ADMINISTERED FIXED DOSE COMBINATION (FDC) PRODUCTS: PHARMACOKINETIC AND BIOPHARMACEUTICAL CONSIDERATIONS
Combination drug therapy has been shown to be beneficial in chronic multifactorial diseases, infectious diseases and in the treatment of comorbid conditions. Some drug combinations have also been developed to improve the bioavailability of the active components by combining with drug metabolizing enzyme inhibitors. Fixed dose combinations (FDC) offer advantages of better efficacy, ease of administ...
Currently there has been an increased interest in using iontophoretic technique for the controlled transdermal drug delivery of medicines, especially in the delivery of proteins & peptides through skin membranes. The delivery of ionic drug molecules and peptide/protein molecules through transdermal delivery to attain a systemic therapeutic effect, chemical or physical methods are needed to imp...
Warfarin, a vitamin K antagonist has been the leading oral anticoagulant since early 1940s despite limitations like slow onset and offset of action, variations in metabolism, multiple food and drug interactions, narrow therapeutic index and need for routine laboratory monitoring. Rivaroxaban, a direct factor Xa inhibitor is a recently introduced orally effective novel anticoagulant offering improv...
MATERNAL MORTALITY: TEN YEARS REVIEW STUDY AT GOA MEDICAL COLLEGE THE ONLY MEDICAL COLLEGE & TEACHING INSTITUTE IN THE STATE OF GOA
Objective: Maternal Mortality: Ten year review study in The Department of Obstetrics and Gynecology, Goa Medical College. Method: A retrospective study was conducted in the Dept. of OBG GMC over a period of10 years spanning between Jan 4 to Dec 13 regarding Maternal Mortality. Results: There were total of 58 deaths out of 54604 live births during this 10 year period giving maternal mortality ratio...
The oral route is easiest and most convenient route for drug administration. The major problem in oral drug formulations is low and erratic bioavailability, which mainly results from poor water solubility. This may leads to higher inter subject and intra subject variability and therapeutic failure. The improvement of compounds solubility and bioavailability are the greatest challenges in formulati...
A new series of s- triazine based chalcones have been synthesized by the temperature controlled reaction. The first substitution occurs at low temperatures (0°C), the second substitution at approximately at room temperature (25-30°C) and the third at elevated temperatures (70-100°C). This property allows substitution of three different neucleophiles onto the same triazine core which provides a ...
PREVENTIVE ANTI-INFLAMMATORY ACTIVITY OF AN AQUEOUS EXTRACT OF LARREA DIVARICATA CAV. AND DIGESTIVE AND HEMATOLOGICAL TOXICITY
Larrea divaricata Cav. commonly known as “jarilla hembra” is used in folklore medicine as anti-inflammatory. There was no scientific evidence justifying the medicinal use of the aqueous extract for inflammation diseases. Nowadays, there are two principal types of anti-inflammatory drugs: Steroidal anti-inflammatory drugs (corticosteroids) and non-steroidal anti-inflammatory drugs (NSAIDs), whi...
POTENTIAL ANTI CANCER ACTIVITY OF SNAKE VENOM, BEE VENOM AND THEIR COMPONENTS IN LIVER AND BREAST CARCINOMA
The present study aimed to evaluate the antitumor efficacy of Snake venom (SV), bee venom (BV), combinations of their components and their apoptotic, cell death mechanisms in liver (HepG2) and breast (MCF7) cell lines. Cytotoxic effects of venoms, L-Amino acid oxidase (svLAAO), phospholipase A2(svPLA2), Melittin ( MEL) were tested against HepG2 and MCF-7 cell lines and IC50 was calculated . mRNA e...
SYNTHESIS AND ANTIMICROBIAL ACTIVITY OF SOME NOVEL 1- PHENYL 1-H-PYRAZOLO[3,4-D]PYRIMIDINE DERIVATIVES
Novel 1- Phenyl 1-H-Pyrazolo[3,4-d]Pyrimidine Derivatives were synthesized from the key intermediate chloro derivative (1).The conversion of chloro derivative(1) into 1,2,4 triazoles, 1,3,4 thiadiazoles and thiazoles was accomplished with the intermediacy of the ester(2) and hydrazide (3). Moreover triazole ring was annelated to the pyrazolopyrmidine core in two step reaction involving reaction wi...
MICRO-ELECTROPHORETIC MOBILITY STUDY OF THE INFLUENCE OF CHLORPROMAZINE HYDROCHLORIDE ON DIMYRSTOYLPHOSPHATIDYLCHOLINE LIPOSOMES IN DIFFERENT MEDIA
In this study, the possible influence of the model, cationic drug chlorpromazine hydrochloride (CPZ-HCl) concentration on the micro-electrophoretic mobility of neutral large multi-lamellar dimyrstoylphosphatidylcholine (DMPC) liposomes was investigated in various media at both 25oC and 37oC. In addition the effect CPZ-HCl concentration on the micro-electrophoretic mobility of both positively charg...
Introduction: Khat is chewed traditionally in Ethiopia, Somalia, Yemen and Kenya. In Ethiopia, khat chewing is now becoming an every-day substance of abuse for the general population regardless of its adverse effects on human health. Therefore, this study was conducted to investigate the effects of crude extract of khat fresh leaves and soft twigs on liver function in rats. Methods: Adult male Wis...
EFFECT OF PROCESS PARAMETERS OF MICROWAVE ASSISTED EXTRACTION (MAE) ON NATURAL PRODUCT YIELD FROM ONION PEEL
Globally, more than 30 % of the onion peels arises at the retail and purchaser levels, of which the post-harvest and dealing out level wastages report for the major share. So, studies on the characterization of unutilized fractions of the onion peels specify their potential candidature for reprocessing. Maximum preservation of these phytochemicals during extraction requires optimised process param...
ANTIMICROBIAL ACTIVITY OF AQUEOUS AND ETHANOL EXTRACTS OF VIOLET PLANT (SECURIDACA LONGIPEDUNCULATA FRES) ON TESTED PATHOGENIC BACTERIA
This study was undertaken to investigate the antimicrobial activities of aqueous and ethanol extracts of violet plant, Securideca longepedunculata leaves and root bark extracts against the pathogenic bacteria isolates of Salmonella typhi, Escherichia coli and Pseudomonas aeroginosa. Disc diffusion method was used for antibacterial testing and Tetracycline drug at 250 mg/ml concentration was used a...
FORMULATION AND EVALUATION OF SOLID SELF-MICROEMULSIFYING DRUG DELIVERY SYSTEM FOR IMPROVED ORAL DELIVERY OF OLMESARTAN MEDOXOMIL
The present work aimed at formulating a solid self micro emulsifying drug delivery system (solid-SMEDDS) for Olmesartan Medoxomil with the objective of improving the aqueous solubility, dissolution and oral delivery of the drug. Liquid SMEDDS of the drug were formulated using Capmul MCM as the oil phase, Tween 80 and Phenoxide HCO-40 as the combined surfactants after screening various vehicles. Th...
PRELIMINARY PHYTOCHEMICAL ANALYSIS AND IN-VITRO HYPOGLYCEMIC POTENTIAL OF NILGIRIANTHUS CILIATUS NEES
Objective: The present study was aimed to investigate the phytochemical analysis and inhibitory activity of Nilgirianthus ciliatus Nees against the carbohydrate hydrolyzing enzymes. Methods: The Nilgirianthus ciliatus plant powder was extracted with ethanol by cold maceration technique. Primary and secondary metabolites present in the plant extract were identified by means of phytochemical screeni...
Objective: Immune-compromised patients are vulnerable to microbes, which are usually safe and sound for healthy individuals. The aim of this study is to provide a quality fresh fruits such as apple, grape, guava, pear and plum for immune-compromised personnel sing different doses of gamma radiation. Methods: Irradiation impacts on microbial number, in treated fruits were assessed and compared to s...
In today's hectic lifestyle, such dosage form as plaster, attracts more attention of scientists and gives new opportunities to use it in medicine. The purpose of our study was to investigate consumer properties of transdermal patches, hold the commodity analysis of the dosage form and determine the prospectiveness of their use in modern pharmacotherapy. Market review has been conducted. Analyzed t...
BIOCHEMICAL, HEMATOLOGICAL AND HISTOLOGICAL CHANGES IN RESPONSE TO GRADED DOSE OF EXTRACT OF EQUISETUM ARVENSE IN ADULT FEMALE WISTAR RATS
Equisetum arvense, commonly known as field horsetail, is a perennial herb belonging to the genus Equisetum. Traditionally, Equisetum arvense is used as diuretic; anticoagulant, skin antiseptic, in kidney and liver related problems and gastric ulcers. E. arvense has also been suggested for the treatment of bone related disorders such as osteoporosis but the data is very scarce on its dose range and...
A SHORT TERM PROSPECTIVE, INTERVENTIONAL, COMPARATIVE CLINICAL STUDY FOR EVALUATION OF RAMIPRIL VERSUS TELMISARTAN IN HYPERTENSIVE PATIENTS WITH TYPE 2 DIABETES MELLITUS
Aim: To comparatively evaluate the antihypertensive efficacy and tolerability of ramipril and telmisartan in stage 1 hypertensive diabetic patients. Material and Methods: This short term, prospective, randomized, interventional, comparative clinical study was conducted amongst 53 patients of stage 1 hypertension with type 2 diabetes (27 ramipril and 26 telmisartan patients), aged 30 years and abov...
EVALUATION OF ANTIDIABETIC POTENTIAL OF METHANOLIC EXTRACT OF BENINCASA HISPIDA IN STREPTOZOTOCIN INDUCED DIABETIC RATS
The number of patients with Diabetes mellitus is increasing rapidly both in developed and developing countries around the world. The existing medications especially synthetic drugs often fail to bring back normal glycemic control without complications. Numerous herbal medicines are widely explored as alternative medicine in diabetes. The objective of this work was to evaluate one such herbal extra...
The disease causing bacteria have evolved the genetic ability to transmit and acquire resistance to drugs used as therapeutic agents. The discovery of antibiotics to combat these pathogens marked a resolution in the 20th century. Unfortunately, because of the inappropriate use of antibiotics in human and veterinary medicine, certain strains of bacteria and fungi developed the ability to produce su...
The present study was done to evaluate in-vitro membrane stabilizing activity with human RBC, in-vivo neuropharmacological activity including open field and swimming test, gastrointestinal motility and acute toxicity of different leaf extracts of Spondias pinnata in Swiss albino mice following oral administration. In-vivo GI motility test was done by charcoal induced anti motility test, acute toxi...
The present work focuses on treatment of glaucoma by formulating ocular inserts of different polymeric combination and Betaxolol to enhance therapeutic effect through prolonging contact time with corneal surface, Betaxolol is a cardio selective (β1 adrenergic) receptor blocking agent. Ophthalmic betaxolol may be especially useful in the treatment of glaucoma in patient with pulmonary disease. Sus...
The bioactive components of Bryonopsis laciniosa fruits have been evaluated using GC/MS. The chemical compositions of the methanolic extract of Bryonopsis laciniosa fruits were investigated using Perkin-Elmer Gas Chromatography –Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. The G...
The mouth of human body provides non-shedding surfaces (teeth) for natural microbial colonization. This can result in the accumulation of large masses of bacteria and their products at stagnant sites. Dental plaque, Periodontitis and gingivitis are some of this type of conditions, which can develop due to microbial accumulation on teeth and Infections of throat leading to tonsillitis, pharyn...
The effect of aqueous leaf extract of Tridax procumbens on blood coagulation was investigated in order to verify the claim that the said extract can be used to manage bleeding. This was done by examining the effect of the extract on bleeding time, the effect of graded amounts of the extract on clotting time as well as the effect of the heat-treated extract on bleeding time. These were performed us...
EFFICIENCY OF DIFFERENT SOLVENTS ON PHYTOCHEMISTRY PROFILE OF WATER MELON (CITRULLUS VULGARIS SCHRAD.) SEED EXTRACTS
Citrullus vulgaris Schrad. has occupied a popular place in the indigenous system of folk medicine because of its high nutritional and antioxidant properties. The study aims to elucidate the extractive yield percentage and screen the seed extracts of watermelon using different solvents for its phytochemical profile. The solvents selected were Methanol, Ethanol, Chloroform, Petroleum ether, Ethyl ac...
LIPOPOLYSACCHARIDE (LPS) AND MURAMYL DIPEPTIDE (MDP) INDUCED CHRONIC LIVER INJURY IN HIGH FAT DIET FED RAT–A POSSIBLE MODEL FOR NON ALCOHOLIC FATTY LIVER DISEASE
Liver defend the PAMPs or DAMPs via regulation of innate immunity through modulation of “TOLL LIKE RECEPTORS and NOD LIKE RECEPTORS” A breakdown of tolerance may induce in appropriate immune response leads to acute and chronic liver diseases like NASH/NAFLD thus considered as disorders of immune response. HFD is itself a basic component for generating metabolic disorders and major source for f...
THE QUALITY ASSESSMENT OF ONCOLOGICAL PAIN MANAGEMENT CLINICAL TRIALS IN THE CONTEXT OF A LIMITED NUMBER OF META-ANALYSIS OF OPIOID FORMULATIONS
Treatment ofcancer painis mainly based on the use of opioidanalgesics.This study includes opioids commonly used in Polish clinical practice, such as: dihydrocodeine, tramadol, buprenorphine, morphine, fentanyl, oxycodone and methadone. The purpose of our research is performing a quality assessment of the available clinical trials concerning opioids efficacy and an evaluation of the cause of the li...
SYNTHESIS, CHARACTERIZATION AND STUDY OF ANTIMICROBIAL ACTIVITY OF 2-HYDROXY 3-NITRO-5-METHYL-N-(SUBSTITUTED PHENYL) CHALCONE IMINES
Chalcones and its derivatives show various biological activities. The present study deals with the synthesis of series of 2-hydroxy-3-nitro-5-methyl–N-substituted phenyl chalcone imines from 2-hydroxy-3-nitro-5-methylphenyl chalcone and different substituted aromatic animes in ethanol in presence of 2, 3 drops of Conc. H2SO4. Structures of synthesized compounds have been established by spectral ...
STABILITY INDICATING ISOCRATIC RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR INDAPAMIDE AND PERINDOPRIL ERBUMINE IN PURE AND ITS COMBINED TABLET DOSAGE FORM
A reverse phase liquid chromatography method (RP-HPLC) was developed to estimate the amount of Indapmide and Perindopril in bulk and its pharmaceutical formulations. A Waters Alliance HPLC system equipped with auto sampler, UV-Visible detector and YMC Column (150 x 4.6mm, 3µ particle size) were used for the quantification of the drugs. Separation was carried out by using potassium dihydrogen phos...
DEVELOPMENT AND EVALUATION OF GASTRORETENTIVE DRUG DELIVERY SYSTEM FOR VENLAFAXINE HYDROCHLORIDE AS BILAYER TABLET
Present research work aims to develop venlafaxine hydrochloride gastro retentive tablets for prolonged release and increased gastric retention time. Venlafaxine is an antidepressant of the serotonin nor- epinephrine reuptake inhibitor (SNRI) class. Bioavailability is 45% following oral administration and half life is 5 hours. To study the effect of formulation variables on drug release and buoyan...
Present investigation deals with the phytochemical and antioxidant studies of Justicia gendarussa Burm.f. (family Acanthaceae), a medicinally important herb. Biochemical studies highlighted that in leaf tissue, quantities of soluble carbohydrate, amino acid and ascorbic acid are of 307 mg/g, 34.93 mg/g and 619µg/g, respectively. In stem bark, quantities of soluble carbohydrate, amino acid and a...
NUTRITIONAL VALUE OF SKELETONEMA COSTATUM (CLEVE, 1873) FROM PARANGIPETTAI, SOUTHEAST COAST OF INDIA
Present aim to study the biochemical composition, i.e., protein, carbohydrate, lipid, ash, amino acids, fatty acids, vitamins, minerals and Carbon, Hydrogen, Nitrogen from Skeletonema costatum. The biomass of marine diatom (S.costatum) were collected from Parangipettai, Southeast cost of India. The lyophilized powder of S.costatum was used for biochemical analysis. The results of proximate composi...
Diabetes mellitus (DM) was found to impair all dimensions of health except mental health and pain and in a more recent multinational study, DM was found to have a notable impact on general health, measured using the Medical Outcomes Short-Form 36 (SF-36). A cross-sectional, randomized study conducted in Ajman and Sharjah, October 2012 to May 2013, using a self-completed questionnaire and SF-36 h...
Objective: The current study was designed to study Acute Dermal Irritation potential of Mastilep gel (supplied by M/S Ayurvet Limited, Baddi, India) according to OECD guidelines. Materials and Methods: Mastilep gel, a new herbal ointment for topical use applied on the udder, has been developed as an alternative method for controlling mastitis in ruminants. Mastilep gel’s active ingredients inclu...
IN-VITRO STUDIES ON OIL DEGRADING BACTERIA ISOLATED FROM OIL CONTAMINATED SOIL OF VANIYAMBADI AND AMBUR AREAS OF VELLORE DISTRICT
The oil polluted soil has many hydrocarbons which can be degraded by microbes. These oil degrading microbes were isolated from the oil contaminated soil through plate and dilution technique. The oil contaminated soils were collected from the oil spilled places of Vaniyambadi and Ambur areas of Vellore District, Tamil nadu, India. Through the biochemical tests, the isolated bacterial strains were i...
The hypolipidemic activity of Cassia tora (Chakvat, Chakunda) (Family: Caesalpiniaceae) seeds extract have been studied in alloxan-induced diabetic rats. In this model oral administration of seed extract (500 mg/kg bw.P.o.) for 15 days in diabetic-dyslipidemic rats exerted significant lipid lowering effect as assessed by the reversal of serum levels of total cholesterol, phospholipids, triglycer...
SYNTHESIS, CHARACTERIZATION AND PHARMACOLOGICAL EVALUATION OF SOME NEW 6-(3,5-SUBSTITUTED-2-BROMO/HYDROXY PHENYL)-1,2,4-TRIAZINE DERIVATIVES AS ANTICONVULSANT AGENTS
6-(2-Amino-3,5-substituted phenyl)-1,2,4-triazines were prepared by refluxing semicarbazones or thiosemicarbazones in presence of sodium hydroxide solution. 6-(3,5-Substituted-2-bromophenyl)-1,2,4-triazine derivatives and 6-(3,5-substituted-2-hydroxyphenyl)-1,2,4-triazine derivatives were prepared from 6-(2-amino-3,5-substituted phenyl)-1,2,4-triazine derivatives by sodium nitrite, hydrobromic aci...
FORMULATION AND CHARACTERIZATION OF CONTROLLED RELEASE BIOADHESIVE NANOPARTICLES ENCAPSULATED WITH NEOSTIGMINE BROMIDE
In the recent days targeted drug delivery has gained more prominence for various advantages like site specific delivery and controlled release from the formulations. Amongst the plethora of avenues explored for targeted drug delivery, bioadhesive nanoparticles backed foremost attention offering local drug delivery and controlled drug release solving problems like tissue damage and drug wastage. Fo...
Bhasmas, the formulations, which can be prepared from metals, minerals or even some marine products, are being used in Ayurvedic system of medicine. Copper (Tamra) is one such metal, used to prepare a bhasma, called “Tamra Bhasma” (TB) which is claimed to cure various diseased conditions. Though bhasmas are prepared since centuries, the exact mechanism of conversion of metal to its bhasma, esp...
Five medicinal plants used in the traditional medicine of India, were selected for studying their immunomodulatory potential through the activation of macrophages, individually and in synergism. The plants were Boswellia serrata Roxb., Curcuma longa L., Glycyrrhiza glabra L., Tribulusterrestris L., and Tylophora indica (Burm f.) Merr. In the present study, strong stimulation of antigen specific an...
ANTIPYRETIC ACTIVITY OF CRUDE AND AQUEOUS EXTRACT OF GULE GHAAFIS (GENTIANA OLIVIERI GRISEB) ON YEAST INDUCED PYREXIA IN ANIMAL MODEL
Ghaafis (Gentiana olivieri Griseb) has been recommended in Classical Unani literature as an important ingredient of many antipyretic formulations. Singularly, it is advocated in both crude and extract form. The present study was conducted to investigate the antipyretic activity of crude and aqueous extract of Gule Ghaafis on yeast induced pyrexia in animal model. The study was carried out by the m...
METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ASPIRIN AND LANSOPRAZOLE IN BULK AND LABORATORY SAMPLE BY DIFFERENT UV SPECTROPHOTOMETRIC TECHNIQUES
Four simple, rapid, inexpensive, precise and accurate UV spectrophotometric methods have been developed for simultaneous estimation of Aspirin (ASP) and Lansoprazole (LANSO). Method A was Simultaneous equation method (Vierodt’s method) which applies measurement of absorptivities at two wavelengths, 276.00 nm, (λmax of Aspirin) and 284.00 nm, (λmax of Lansoprazole) in zero order spectra. The co...
CLINICAL OUTCOME STUDY OF CRITICALLY-ILL SEPTIC PATIENTS GIVEN TAURINE SUPPLEMENTED ENTERAL NUTRITION
Background: Sepsis is a significant public health concern and is the main cause of death in surgical intensive care units (ICUs). Patients with sepsis have features consistent with immunosuppression. Taurine is rapidly emerging as one of the more interesting amines which has been reported to have immune-modulatory effect through its action on cytokines. Purpose: To determine the effect of using Im...
INVESTIGATION ON IN VITRO CYTOTOXIC ACTIVITY OF A SELECTED WILD CUCURBITACEAE PLANT CORALLOCARPUS EPIGEAUS AGAINST CANCER
Medicinal plants have been investigated for possible anti-cancer effects. Corallocarpus epigaeus (Roth & willd.) cl. is an ethnobotanically important wild climber that belongs to the family Cucurbitaceae. The aim of the present study is to examine the cytotoxic activity of Corallocarpus epigaeus (Roth & willd.) cl., on two tumor cell lines. The methanolic root extract of the plant Coralloc...
In the present investigation, controlled release microparticles of Glimepiride were prepared by emulsion solvent evaporation technique using cellulose acetate as release retardant polymer. The microparticles were evaluated for In-Vitro release study and In-Vivo evaluation. The microparticles were subjected to In-vitro release studies by employing 7.8 pH phosphate buffer as dissolution medium. The ...
DIVERSITY OF MOLLUSCAN FAUNA FROM FRESHWATER BODIES OF SANGLI DISTRICT: A COMPREHENSIVE STUDY IN RELATION TO ENVIRONMENTAL VARIABLES
Molluscs as a second largest phyla includes snails, slugs, clams, oysters, scallops, cuttlefish, squid and octopus. Present study revealed, diversity of molluscan fauna from three sites i.e Khanderajuri lake, Mhaishal lake and Krishna river in the period of January, 2011 to December, 2013. Among collected molluscs 10 species were identified from seven families i.e. Physidae, Lymnaeidae, Viviparida...
The aim of present work was to develop and evaluate solid lipid nanoparticles (SLNs) based gel for topical delivery of antifungal agent. Bifonazole (BFZ) is an imidazole antifungal agent having broad spectrum activity against dermatophytes, moulds, yeasts, fungi and some gram positive bacteria. BFZ SLNs systems were developed by melt emulsification followed by ultrasonication technique using Preci...
IMPROVING THE PERFORMANCE OF PHARMACEUTICAL TABLET PRODUCTION USING SIX SIGMA METHODOLOGY (MODULATION ON DIGESTIVE ENZYME TABLET)
Each company is expected to develop specifications for their products to be accommodated with the inspection guide of pharmaceutical quality control. After product analysis for (X) pharmaceutical company it was noticed that 84.5% of defects were attributed to tablet department while 14.1% and 1.4% for ampoule and syrup departments respectively so it was valuable to solve the tablet department prob...
METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF TINIDAZOLE BY REVERSE PHASE HPLC TECHNIQUE
The aim of the current study is to develop a simple, specific, rapid and precise quantification technique for the estimation of tinidazole from tablet dosage form. Successful separation of the drug was carried out on a C18 column (particle size 5 µm, 250 mm length × 4 mm i.d.) using a mobile phase consisting of a 5.3 mM phosphate buffer solution and acetonitrile in the ratio of 60:40 (v/v). The ...
ISOLATION AND IDENTIFICATION OF L-ASPARAGINASE PRODUCING SALINICOCCUS SP. M KJ997975 FROM SOIL MICROBIAL FLORA
L-asparaginase is responsible for the hydrolysis of L-asparagine into L-aspartic acid and ammonia. L-asparaginase is used as an effective therapeutic agent against lymphocytic leukemia. It also finds applications in food industry. Though much has been unraveled about LA, it may appears today that it is at the tip of iceberg and there is tremendous scope in screening of novel LA sources and studyin...
EFFECT OF NOVEL BENZISOXAZOLE DERIVATIVES AGAINST EHRLICH ASCITES CARCINOMA CELLS IN SWISS ALBINO MICE: CYTOTOXIC AND HAEMATALOGICAL STUDIES
The present study is to investigate the cytotoxicity properties and haematological indices of synthesized molecules (S1-S4) in comparison to the standard drug 5 fluorouracil which reflects the anticancer potentials of the above molecules. Cytotoxicity studies were performed by in vitro MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyl teterazolium bromide) assay. Haematological studies were done b...
HPLC-ELSD DETERMINATION OF SODIUM LAURYL SULPHATE AND POLYSORBATE IN NEBIVOLOL DRUG PRODUCT AND DIFFERENT FORMULATION PRODUCTS
The contents of Sodium lauryl sulphate (SLS) and Polysorbate (Tween 80) were studied in different formulations of Nebivolol film coated tablets. The contents were extracted using the diluent Methanol: Water in the ratio 60:40 v/v and analyzed in HPLC coupled with Evaporative light scattering detector (ELSD). SLS and Tween 80 are solubilizing agents and the amount of these contents are critical in ...
The present study aims to explore the ethnomedicinal knowledge of the plants and their preparations used to cure diabetes by the tribes of Mananthavady, Bathery and Vythiri taluks of Wayanad district, Kerala. Preparation of ethnomedicine using various plant parts and other information related to tribes associated; habitat, habit and status of medicinal plants recorded are highlighted. Documentatio...
BACOPAMONNIERA, A NOOTROPIC HERB ALLEVIATES ABNORMAL MOLECULAR AND IMMUNOHISTOCHEMICAL CHANGES EVOKED BY CHRONIC EXPOSURE OF METHYLMERCURY IN RAT CEREBELLUM
Methylmercury (MeHg), a highly toxic environmental pollutant, induces oxidative stress and dysfunction in many cell types. Bacopa monniera is a perennial herb, and is used as a nerve tonic in ayurveda, a traditional medicinal system in India. This study was aimed to evaluate the effect of Bacopa monniera extract (BME) on MeHg-induced toxicity in rat cerebellum. Male Wistar rats were administered w...
ANTIHYPERCHOLESTROLEMIC POTENTIAL OF OMEGA-3-FATTY ACID CONCENTRATE IN ALLOXAN INDUCED DIABETIC RODENT
Exhaustive study of scientific literature provides the initial evidence that omega-3-fatty acids the principal component of fish oil is responsible for exerting important biological effects when administered to population at risk of coronary heart diseases and diabetes mellitus. The main reason behind the study was to evaluate the anti-atherogenic properties of marine lipids in alloxan induced dia...